Chromosomal analysis and identification based on optical tweezers and Raman spectroscopy / Jenifer Fay Ojeda.
| Author/creator | Ojeda, Jenifer Fay author. |
| Other author | McConnell, Thomas J., degree supervisor. |
| Other author | East Carolina University. Department of Biology. |
| Format | Theses and dissertations |
| Production | 2005. |
| Description | 204 leaves : illustrations (some color) ; 29 cm |
| Supplemental Content | Access via ScholarShip |
| Subjects |
| Summary | Chromosomes are structures that consist of a variety of ever-changing molecules. The proteins that interact with the chromatin of a chromosome at any one stage of gene regulation has long been a mystery due to the complications associated with real-time data collection at the macromolecular level. Raman spectroscopy is an excellent tool for collecting this kind of information, and it is important to begin these analyses by collecting data about the chromosomes as a whole. The purpose of this study was to determine if Raman spectroscopy patterns for each chromosome number 1,2, and 3 could differentiate these three chromosomes. First, the chromosomes were harvested using a hypotonic solution followed by cell lysis with detergent. The chromosomes remained suspended in lysis buffer and were placed dropwise onto a special glass slide. Optical tweezers were then used to manipulate the individual chromosome away from others and a Raman spectral pattern was obtained with the assistance of the Origins 5.0 computer program. These patterns were collected for chromosome numbers 1, 2, and 3, along with pictures of each. The use of Laser Tweezer Raman Spectroscopy (LTRS) allowed the chromosome to then be manipulated to another slide well with the optical tweezers and finally G -banded for positive identification. The Raman patterns of each chromosome were then compared to one another in order to demonstrate the similarities between all of the patterns from a certain chromosome. The Raman patterns were also compared between the chromosomes and various analyses of these spectra showed differences between each chromosome, suggesting that each chromosome number has a distinguishable Raman spectral pattern. The variability between each spectra within a chromosome may lend itself to more detailed studies of gene regulation based on histone interactions with other proteins and the resultant chromatin structure. |
| General note | Presented to the faculty of the Department of Biology. |
| General note | Advisor: Thomas J. McConnell |
| Dissertation note | M.S. East Carolina University 2005 |
| Bibliography note | Includes bibliographical references (leaves 53-56). |
| Genre/form | dissertations. |
| Genre/form | Academic theses. |
| Genre/form | Academic theses. |
| Genre/form | Thèses et écrits académiques. |
Availability
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|---|---|---|---|---|
| Joyner | University Archives | ASK AT SPECIAL COLLECTIONS DESK | ✔ Available | Request Material |
| Electronic Resources | Access Content Online | ✔ Available |