Tools

LEADER 03345ctm 2200529 i 4500

001 ocm70880042;

003 OCoLC;

005 20260505033828.0;

008 060811s2005 xx a bm 000 0 eng d;

035 a| (OCoLC)70880042;

050 4 a| RA1242.A36b| S29 2005;

049 a| EREE;

100 1 a| Sayer, Andrew N.,e| author.?| UNAUTHORIZED;

245 10 a| Brevetoxin toxicity in mammalian cells as influenced by brevenal /c| by Andrew N. Sayer.;

264 0 c| 2005.;

300 a| 74 leaves :b| illustrations ;c| 28 cm;

336 a| textb| txt2| rdacontent;

337 a| unmediatedb| n2| rdamedia;

338 a| volumeb| nc2| rdacarrier;

502 b| M.S.c| East Carolina Universityd| 2005;

500 a| Presented to the faculty of the Department of Biology.;

500 a| Advisor: James E. Gibson;

520 3 a| Brevetoxins (PbTx) are highly potent polyether toxins produced during blooms of several species of marine dinoflagellates, most notably Karenia brevis. Brevenal is a nontoxic short-chain polyether that competes with PbTx for the active site on voltage-sensitive sodium charmels. Human consumption of shellfish contaminated with PbTxs results in neurotoxic shellfish poisoning (NSP). Additionally, blooms of K. brevis are potentially responsible for adverse human health effects such as respiratory irritation and airway constriction in coastal residents. The purpose of this study was to determine if PbTx could induce DNA damage in mammalian cells, and if so, if the damage could be antagonized by brevenal. Results from the single-cell gel electrophoresis assay, inhibition of cellular proliferation assay, chromosomal aberration assay, and disruption of signaling pathways via a Western Blot analysis demonstrate that PbTx is a potent inducer of mammalian cell damage, an effect which can be reduced or alleviated by brevenal.;

504 a| Includes bibliographical references (leaves 70-74)).;

650 0 a| Toxic marine algaex| Health aspects.=| ^A685153;

650 0 a| Polyethers.=| ^A722469;

650 0 a| Dinoflagellate blooms.=| ^A476469;

650 0 a| Red tide.=| ^A591518;

650 7 a| Dinoflagellate blooms.2| fast0| (OCoLC)fst00893973;

650 7 a| Polyethers.2| fast0| (OCoLC)fst01070434;

650 7 a| Red tide.2| fast0| (OCoLC)fst01092190;

655 2 a| Academic Dissertation0| (DNLM)D019478?| UNAUTHORIZED;

655 7 a| Academic theses.2| fast0| (OCoLC)fst01726453;

655 7 a| Academic theses.2| lcgft;

655 7 a| Thèses et écrits académiques.2| rvmgf0| (CaQQLa)RVMGF-000001173;

700 1 a| Gibson, James E.,d| 1941-2025,e| degree supervisor.?| UNAUTHORIZED;

710 2 a| East Carolina University.b| Department of Biology.=| ^A637467;

856 41 z| Access via ScholarShipu| http://hdl.handle.net/10342/12751;

949 a| Click on web addressw| asish| joyner101;

949 a| Click on web addressw| asish| hsl111;

994 a| C0b| ERE;

596 a| 1 4;

998 a| 1051509;

999 a| ASK AT SPECIAL COLLECTIONS DESKw| ASISc| 1i| MQ1838577l| JSCJTHDm| JOYNERr| Ys| Yt| JSCJTHDu| 8/11/2006x| ETDz| JSPECCOLLo| .STAFF. 0;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 1051509-3001l| JNETm| JOYNERr| Ys| Yt| JNE3ETDu| 5/5/2026x| ETDz| JERESOURCE;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 1051509-4001l| HSLELECm| HSLr| Ys| Yt| HEETDu| 5/5/2026x| ETDz| HERESOURCE;

Brevetoxin toxicity in mammalian cells as influenced by brevenal / by Andrew N. Sayer.

Author/creator	Sayer, Andrew N. author.
Other author	Gibson, James E., 1941-2025, degree supervisor.
Other author	East Carolina University. Department of Biology.
Format	Theses and dissertations
Production	2005.
Description	74 leaves : illustrations ; 28 cm
Supplemental Content	Access via ScholarShip
Subjects	Toxic marine algae--Health aspects. Polyethers. Dinoflagellate blooms. Red tide.

Summary	Brevetoxins (PbTx) are highly potent polyether toxins produced during blooms of several species of marine dinoflagellates, most notably Karenia brevis. Brevenal is a nontoxic short-chain polyether that competes with PbTx for the active site on voltage-sensitive sodium charmels. Human consumption of shellfish contaminated with PbTxs results in neurotoxic shellfish poisoning (NSP). Additionally, blooms of K. brevis are potentially responsible for adverse human health effects such as respiratory irritation and airway constriction in coastal residents. The purpose of this study was to determine if PbTx could induce DNA damage in mammalian cells, and if so, if the damage could be antagonized by brevenal. Results from the single-cell gel electrophoresis assay, inhibition of cellular proliferation assay, chromosomal aberration assay, and disruption of signaling pathways via a Western Blot analysis demonstrate that PbTx is a potent inducer of mammalian cell damage, an effect which can be reduced or alleviated by brevenal.
General note	Presented to the faculty of the Department of Biology.
General note	Advisor: James E. Gibson
Dissertation note	M.S. East Carolina University 2005
Bibliography note	Includes bibliographical references (leaves 70-74)).
Genre/form	Academic theses.
Genre/form	Academic theses.
Genre/form	Thèses et écrits académiques.

Availability

Library	Location	Call Number	Status	Item Actions
Joyner	University Archives	ASK AT SPECIAL COLLECTIONS DESK	✔ Available	Request Material
	Electronic Resources	Access Content Online	✔ Available

Brevetoxin toxicity in mammalian cells as influenced by brevenal / by Andrew N. Sayer.

Click here for more information about this title

Availability