Signal transduction protocols / edited by Robert C. Dickson and Michael D. Mendenhall.

Other author Dickson, Robert C.
Other author Mendenhall, Michael Dean.
Format Book
Edition2nd ed.
Publication InfoTotowa, N.J. : Humana Press, ©2004.
Descriptionxii, 327 pages : illustrations ; 24 cm.
Supplemental ContentTable of contents
Subjects

SeriesMethods in molecular biology ; 284
Methods in molecular biology (Clifton, N.J.) v. 284. ^A682607
Contents Making protein immunoprecipitates / Elaine A. Elion and Yunmei Wang -- Signal transduction inhibitors in cellular function / Maofu Fu ... [et al.] -- Two-dimensional gel electrophoresis for the identification of signaling targets / Yukihito Kabuyama ... [et al.] -- A high-throughput mammalian cell-based transient transfection assay / Daniel J. Noonan, Kenneth Henry, and Michelle L. Twaroski -- Determining protein half-lives / Pengbo Zhou -- Assaying protein kinase activity / Jan Brábek and Steven K. Hanks --
Contents Comparative phosphorylation site mapping from gel-derived proteins using a multidimensional ES/MS-based approach / Francesca Zappacosta, Michael J. Huddleston and Roland S. Annan -- Studies of calmodulin-dependent regulation / Paul C. Brandt and Thomas C. Vanaman -- Measurement of protein-DNA interactions in vivo by chromatin immunoprecipitation -- Hogune Im ... [et al.] --
Contents Characterization of protein-DNA association in vivo by chromatin immunoprecipitation / Laurent Kuras -- Nonradioactive methods for detecting activation of Ras-related small G proteins / Douglas A. Andres -- Nucleocytoplasmic glycosylation, O-GlcNAc: identification and site mapping / Natasha Elizabeth Zachara, Win Den Cheung, and Gerald Warren Hart --
Contents Techniques in protein methylation / Jaeho Lee, Donghang Cheng, and Mark T. Bedford -- Assaying lipid phosphate phosphatase activities / Gil-Soo Han and George M. Carman -- Assaying phosphoinositide phosphatases / Gregory S. Taylor and Jack E. Dixon -- Assaying phospholipase A² activity / Christina C. Leslie and Michael H. Gelb --
Contents Measurement and immunofluorescence of cellular phosphinositides / Hiroko Hama ... [et al.] -- Measuring dynamic changes in cAMP using fluorescence resonance energy transfer / Sandrine Evellin ... [et al.] -- In vivo detection of protein-protein interaction in plant cells using BRET / Chitra Subramanian ... [et al.] -- Revealing protein dynamics by photobleaching techniques / Frank van Drogen and Matthias Peter -- Assaying cytochrome c translocation during apoptosis / Nigel J. Waterhouse ... [et al.].
Bibliography noteIncludes bibliographical references and index.
LCCN 2004005164
ISBN1588292452 (alk. paper)
ISBN1592598161 (E-ISBN)

Availability

Library Location Call Number Status Item Actions
Joyner General Stacks QP517.C45 S555 2004 ✔ Available Place Hold