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LEADER 03540ctm 2200541 i 4500

001 ocm71358242;

003 OCoLC;

005 20260420152327.0;

008 060919s2004 xx a bm 000 0 eng d;

035 a| (Sirsi) o71358242;

035 a| (OCoLC)71358242;

049 a| EREE;

050 4 a| QL979.5b| .W55 2004;

100 1 a| Williams, Dennis Ray,e| author.;

245 13 a| An in vitro analysis of chondrogenesis utilizing the versican deficient hdf mutant mouse /c| by Dennis Ray Williams, Jr.;

264 0 c| 2004.;

300 a| 56 leaves :b| illustrations (some color) ;c| 28 cm;

336 a| textb| txt2| rdacontent;

337 a| unmediatedb| n2| rdamedia;

338 a| volumeb| nc2| rdacarrier;

502 b| M.S.c| East Carolina Universityd| 2004;

500 a| Presented to the faculty of the Department of Biology.;

500 a| Advisor: Anthony A. Capehart;

520 3 a| Mesenchymal condensation or aggregation represents a pivotal process during endochondral skeletogenesis. Extracellular matrix (ECM) interactions have been previously reported to play a crucial role in the process of mesenchymal aggregation and subsequent cartilage differentiation. It has been suggested that the modular ECM proteoglycan, versican, plays a significant role in early events of skeletogenesis by virtue of its spatiotemporal expression pattern during mesenchymal aggregation and chondrogenesis. The versican-deficient hdf transgenic mouse has provided the first model to explore the implications of a null mature versican on chondrogenesis. Due to embryonic lethality of hdf homozygotes at 10.5 dpc, the well accepted high-density micromass culture system was employed to compare the in vitro chondrogenic capacity of hdf mutants to that of the wild type. Mesenchyme derived from hdf homozygous mutants demonstrated an inhibition of mesenchymal aggregation and an inability to produce cartilage characteristic molecules after 6 days of high-density micromass culture. Results of this study strongly suggest that versican is essential for mesenchymal aggregation and subsequent cartilage differentiation.;

504 a| Includes bibliographical references (leaves 39-46).;

650 0 a| Chondrogenesis.;

650 0 a| Proteoglycans.;

650 0 a| Mice as laboratory animals.;

650 2 a| Proteoglycans.0| (DNLM)D011509;

655 2 a| Academic Dissertation.0| (DNLM)D019478;

650 7 a| Chondrogenesis.2| fast0| (OCoLC)fst00858483;

650 7 a| Mice as laboratory animals.2| fast0| (OCoLC)fst01019388;

650 7 a| Proteoglycans.2| fast0| (OCoLC)fst01079778;

655 7 a| Academic theses.2| fast0| (OCoLC)fst01726453;

655 7 a| Academic theses.2| lcgft;

655 7 a| Thèses et écrits académiques.2| rvmgf0| (CaQQLa)RVMGF-000001173;

700 1 a| Capehart, Anthony A.,e| degree supervisor.;

710 2 a| East Carolina University.b| Department of Biology.;

856 41 z| Access via ScholarShipu| http://hdl.handle.net/10342/12916;

949 a| Click on web addressw| asish| joyner101;

949 a| Click on web addressw| asish| hsl111;

994 a| C0b| ERE;

596 a| 1 4;

998 a| 1055796;

999 a| ASK AT SPECIAL COLLECTIONS DESKw| ASISc| 1i| MQ1858525l| JSCJTHDm| JOYNERr| Ys| Yt| JSCJTHDu| 9/19/2006x| ETDz| JSPECCOLLo| .STAFF. 0;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 1055796-3001l| JNETm| JOYNERr| Ys| Yt| JNE3ETDu| 4/14/2026x| ETDz| JERESOURCE;

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An in vitro analysis of chondrogenesis utilizing the versican deficient hdf mutant mouse / by Dennis Ray Williams, Jr.

Author/creator	Williams, Dennis Ray author.
Other author	Capehart, Anthony A., degree supervisor.
Other author	East Carolina University. Department of Biology.
Format	Theses and dissertations
Production	2004.
Description	56 leaves : illustrations (some color) ; 28 cm
Supplemental Content	Access via ScholarShip
Subjects	Chondrogenesis. Proteoglycans. Mice as laboratory animals. Proteoglycans.

Summary	Mesenchymal condensation or aggregation represents a pivotal process during endochondral skeletogenesis. Extracellular matrix (ECM) interactions have been previously reported to play a crucial role in the process of mesenchymal aggregation and subsequent cartilage differentiation. It has been suggested that the modular ECM proteoglycan, versican, plays a significant role in early events of skeletogenesis by virtue of its spatiotemporal expression pattern during mesenchymal aggregation and chondrogenesis. The versican-deficient hdf transgenic mouse has provided the first model to explore the implications of a null mature versican on chondrogenesis. Due to embryonic lethality of hdf homozygotes at 10.5 dpc, the well accepted high-density micromass culture system was employed to compare the in vitro chondrogenic capacity of hdf mutants to that of the wild type. Mesenchyme derived from hdf homozygous mutants demonstrated an inhibition of mesenchymal aggregation and an inability to produce cartilage characteristic molecules after 6 days of high-density micromass culture. Results of this study strongly suggest that versican is essential for mesenchymal aggregation and subsequent cartilage differentiation.
General note	Presented to the faculty of the Department of Biology.
General note	Advisor: Anthony A. Capehart
Dissertation note	M.S. East Carolina University 2004
Bibliography note	Includes bibliographical references (leaves 39-46).
Genre/form	Academic theses.
Genre/form	Academic theses.
Genre/form	Thèses et écrits académiques.

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An in vitro analysis of chondrogenesis utilizing the versican deficient hdf mutant mouse / by Dennis Ray Williams, Jr.

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