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001 ocm84842682;

003 OCoLC;

005 20221129104748.0;

008 070222s2004 xx ak bm 000 0 eng d;

035 a| (Sirsi) o84842682;

035 a| (OCoLC)84842682;

049 a| EREE;

050 4 a| QP609.A53b| G46 2004;

100 1 a| George, Sarah C.,e| author.?| UNAUTHORIZED;

245 14 a| The effect of L-NAME administration on angiogenesis in overloaded, hyperthrophying rat skeletal muscle /c| by Sarah C. George.;

264 0 c| 2004.;

300 a| vi, 71 leaves :b| illustrations, forms ;c| 28 cm;

336 a| textb| txt2| rdacontent;

337 a| unmediatedb| n2| rdamedia;

338 a| volumeb| nc2| rdacarrier;

502 b| M.A.c| East Carolina Universityd| 2004;

500 a| Presented to the faculty of the Department of Exercise and Sport Science.;

500 a| Advisor: Scott E. Gordon;

520 3 a| Skeletal muscle capillary proliferation is known to occur in response to an overload hypertrophy stimulus. Nitric oxide is necessary for muscle capillary proliferation in an aerobic exercise model; however, nitric oxide's role in capillary proliferation in overloaded skeletal muscle is unknown. Thus, the aim of this investigation was to determine to effect of Nw-nitro-L-arginine methyl ester L-NAME (a nitric oxide synthase inhibitor) administration on capillary angiogenesis in overloaded, hypertrophying rat skeletal muscle. Adult female Sprague Dawley rats (200-225g) were weight matched into either a control group (n=8) or L-NAME group (n=10). Twenty-one days of functional overload of the plantaris muscle in the left leg was produced by unilateral surgical ablation of the synergistic gastrocnemius muscle, while the right leg of each animal underwent a sham operation. A dosage of 20.9±0.3 mg/kg BW/day of L-NAME was administered to the L-NAME group two days prior to surgery and continuing throughout the loading period. After the loading period, plantaris muscles were harvested, analyzed for wet weight and total protein content, and subjected to immunohistochemical staining for capillarity and fiber size in 10pm cross-sections. Although plantaris mass, protein content, fiber cross-sectional area, and fiber perimeter were all lower in the L-NAME group, all of these variables increased to an equal relative extent with overload in both groups. In contrast, increases in capillary contacts per fiber and capillaries per fiber due to overload were observed only in the control group. L-NAME administration prevented the increase in capillarity due to the overload stimulus in rat plantaris muscles, indicating that nitric oxide is important for the angiogenic response observed in overloaded, hypertrophying skeletal muscle.;

504 a| Includes bibliographical references (leaves 47-53).;

650 0 a| Angiotensin converting enzymex| Inhibitors.=| ^A588276;

650 0 a| Musculoskeletal systemx| Hypertrophy.=| ^A4330;

650 0 a| Rats as laboratory animals.=| ^A10876;

650 7 a| Angiotensin converting enzymex| Inhibitors.2| fast0| (OCoLC)fst00808853;

650 7 a| Rats as laboratory animals.2| fast0| (OCoLC)fst01090353;

655 7 a| Academic theses.2| fast0| (OCoLC)fst01726453;

655 7 a| Academic theses.2| lcgft;

655 7 a| Thèses et écrits académiques.2| rvmgf0| (CaQQLa)RVMGF-000001173;

700 1 a| Gordon, Scott Edward,e| degree supervisor.?| UNAUTHORIZED;

710 2 a| East Carolina University.b| Department of Exercise and Sport Science.?| UNAUTHORIZED;

856 41 z| Access via ScholarShipu| http://hdl.handle.net/10342/10393;

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998 a| 1071358;

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The effect of L-NAME administration on angiogenesis in overloaded, hyperthrophying rat skeletal muscle / by Sarah C. George.

Author/creator	George, Sarah C. author.
Other author	Gordon, Scott Edward, degree supervisor.
Other author	East Carolina University. Department of Exercise and Sport Science.
Format	Theses and dissertations
Production	2004.
Description	vi, 71 leaves : illustrations, forms ; 28 cm
Supplemental Content	Access via ScholarShip
Subjects	Angiotensin converting enzyme--Inhibitors. Musculoskeletal system--Hypertrophy. Rats as laboratory animals.

Summary	Skeletal muscle capillary proliferation is known to occur in response to an overload hypertrophy stimulus. Nitric oxide is necessary for muscle capillary proliferation in an aerobic exercise model; however, nitric oxide's role in capillary proliferation in overloaded skeletal muscle is unknown. Thus, the aim of this investigation was to determine to effect of Nw-nitro-L-arginine methyl ester L-NAME (a nitric oxide synthase inhibitor) administration on capillary angiogenesis in overloaded, hypertrophying rat skeletal muscle. Adult female Sprague Dawley rats (200-225g) were weight matched into either a control group (n=8) or L-NAME group (n=10). Twenty-one days of functional overload of the plantaris muscle in the left leg was produced by unilateral surgical ablation of the synergistic gastrocnemius muscle, while the right leg of each animal underwent a sham operation. A dosage of 20.9±0.3 mg/kg BW/day of L-NAME was administered to the L-NAME group two days prior to surgery and continuing throughout the loading period. After the loading period, plantaris muscles were harvested, analyzed for wet weight and total protein content, and subjected to immunohistochemical staining for capillarity and fiber size in 10pm cross-sections. Although plantaris mass, protein content, fiber cross-sectional area, and fiber perimeter were all lower in the L-NAME group, all of these variables increased to an equal relative extent with overload in both groups. In contrast, increases in capillary contacts per fiber and capillaries per fiber due to overload were observed only in the control group. L-NAME administration prevented the increase in capillarity due to the overload stimulus in rat plantaris muscles, indicating that nitric oxide is important for the angiogenic response observed in overloaded, hypertrophying skeletal muscle.
General note	Presented to the faculty of the Department of Exercise and Sport Science.
General note	Advisor: Scott E. Gordon
Dissertation note	M.A. East Carolina University 2004
Bibliography note	Includes bibliographical references (leaves 47-53).
Genre/form	Academic theses.
Genre/form	Academic theses.
Genre/form	Thèses et écrits académiques.

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The effect of L-NAME administration on angiogenesis in overloaded, hyperthrophying rat skeletal muscle / by Sarah C. George.

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