The effect of versican (Cspg-2) on the localization of myoblasts and axons in the developing limb in vitro / by A. Todd Richmond.
| Author/creator | Richmond, A. Todd author. |
| Other author | Capehart, Anthony A., degree supervisor. |
| Other author | East Carolina University. Department of Biology. |
| Format | Theses and dissertations |
| Production | 2004. |
| Description | 63 leaves : illustrations (some color) ; 28 cm |
| Supplemental Content | Access via ScholarShip |
| Subjects |
| Subject | Only the lateral plate mesoderm is native to the developing limb while skeletal myoblasts and axons migrate into the limb from the somites and neural tube. While the migratory nature of these cell types is well documented, questions regarding how they migrate to their appropriate location remain unanswered. The extracellular matrix proteoglycan versican {Cspg-2) has been suggested to act as an inhibitory cue to muscle and nerve migration, thus facilitating proper patterning of these cell types in the embryonic limb. In the present study, in vitro comparison of hclf (heart defect) mouse limb mesenchyme, which bears an insertional mutation in the versican core protein, with wild type limb mesenchyme was used to examine versican influences on these migratory cells. After three days, wild type myoblasts showed a statistically significant avoidance of cellular aggregates strongly expressing versican while hdf mutant myoblasts demonstrated an abnormal phenotype and tended to cluster within hdf mutant cultures. Hdf mutant and wild type limb mesenchyme cultured on both sides of micropore filters showed that in the absence of mature versican, wild type myoblasts did not avoid any regions of the cell layer. Axons from wild type neural tubes avoided cellular aggregates strongly expressing versican after five days of co-culture. Likewise, wild type neural tube cultured with mutant mesenchyme demonstrated that axons would migrate within all regions of the host culture. Versican in aggregating limb mesenchyme may therefore limit the migration of both skeletal myoblasts and axons in vitro aiding in their ultimate patterning in the developing limb. |
| General note | Presented to the faculty of the Department of Biology. |
| General note | Advisor: Anthony A. Capehart |
| Dissertation note | M.S. East Carolina University 2004 |
| Bibliography note | Includes bibliographical references (leaves 55-63). |
| Genre/form | Academic theses. |
| Genre/form | Academic theses. |
| Genre/form | Thèses et écrits académiques. |
Availability
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|---|---|---|---|---|
| Joyner | University Archives | ASK AT SPECIAL COLLECTIONS DESK | ✔ Available | Request Material |
| Electronic Resources | Access Content Online | ✔ Available |