Tools

LEADER 03964ctm 2200553 i 4500

001 ocn166229910;

003 OCoLC;

005 20251021154320.0;

008 070821s2004 xx a bm 000 0 eng d;

035 a| (Sirsi) o166229910;

035 a| (OCoLC)166229910;

043 a| n-us-nc;

049 a| EREE;

050 4 a| QK495.C1153b| M66 2004;

100 1 a| Moore, Shawn Anthony,e| author.?| UNAUTHORIZED;

245 10 a| Determination of optimal concentrations of Cassia occidentalis used to inhibit histamine release from MC/9 mast cells /c| by Shawn Anthony Moore.;

264 0 c| 2004.;

300 a| xi, 70 leaves :b| illustrations ;c| 28 cm;

336 a| textb| txt2| rdacontent;

337 a| unmediatedb| n2| rdamedia;

338 a| volumeb| nc2| rdacarrier;

502 b| M.S.c| East Carolina Universityd| 2004;

500 a| Presented to the faculty of the Department of Biology.;

500 a| Advisor: Gerhard W. Kalmus;

520 3 a| The present study focused on the potential anti-inflammatory effects of Cassia occidentalis, a plant used medicinally as a treatment for rheumatism and acute inflammation, native to the Old World tropics; however, it is also native to North Carolina. To verify the anti-inflammatory property of C. occidentalis, the release of histamine from MC/9 mast cells by treatment with various extract concentrations was quantitatively measured. Leaves of C. occidentalis were removed and crushed into a powder. This powder underwent a Soxhlet extraction using methylene chloride or absolute methanol. The MC/9 cells were activated to release histamine using Compound 48/80, a mast cell stimulator. Total histamine release was assayed by an Enzyme-Linked Immunosorbent Assay (ELISA). Results indicate that C. occidentalis extracts at 1x104 æg/ml, extracted with methylene chloride, has the ability to inhibit histamine release. Additionally, C. occidentalis extracts at 1x101 æg/ml, extracted with absolute methanol, also inhibits the histamine release from MC/9 cells; however, cells washed once to remove excess extract showed stronger histamine inhibition than cells washed multiple times. Furthermore, C. occidentalis extract, using methylene chloride at 1x104 mcg/ml, has a similar inhibitory effect as the commercially available mast cell stabilizer. Cromolyn, at a therapeutic dose of 2x104 mcg/ml.;

504 a| Includes bibliographical references (leaves 66-70).;

650 0 a| Cassia (Genus)z| North Carolina.=| ^A909772;

650 0 a| Anti-inflammatory agents.=| ^A2401;

650 0 a| Histamine.=| ^A7437;

650 7 a| Anti-inflammatory agents.2| fast0| (OCoLC)fst00810354;

650 7 a| Cassia (Genus)2| fast0| (OCoLC)fst00848335;

650 7 a| Histamine.2| fast0| (OCoLC)fst00957647;

655 7 a| dissertations.2| aat0| (CStmoGRI)aatgf300028029;

655 7 a| Academic theses.2| fast0| (OCoLC)fst01726453;

655 7 a| Academic theses.2| lcgft;

655 7 a| Thèses et écrits académiques.2| rvmgf0| (CaQQLa)RVMGF-000001173;

700 1 a| Kalmus, Gerhard W.,e| degree supervisor.?| UNAUTHORIZED;

710 2 a| East Carolina University.b| Department of Biology.=| ^A637467;

856 41 z| Access via ScholarShipu| http://hdl.handle.net/10342/12615;

949 a| Click on web addressw| asish| joyner101;

949 a| Click on web addressw| asish| hsl111;

994 a| C0b| ERE;

596 a| 1 4;

998 a| 1090705;

999 a| ASK AT SPECIAL COLLECTIONS DESKw| ASISc| 1i| MQ1932563l| JSCJTHDm| JOYNERr| Ys| Yt| JSCJTHDu| 8/21/2007x| ETDz| JSPECCOLLo| .STAFF. 0;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 1090705-3001l| JNETm| JOYNERr| Ys| Yt| JNE3ETDu| 8/20/2025x| ETDz| JERESOURCE;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 1090705-4001l| HSLELECm| HSLr| Ys| Yt| HEETDu| 8/20/2025x| ETDz| HERESOURCE;

Determination of optimal concentrations of Cassia occidentalis used to inhibit histamine release from MC/9 mast cells / by Shawn Anthony Moore.

Author/creator	Moore, Shawn Anthony author.
Other author	Kalmus, Gerhard W., degree supervisor.
Other author	East Carolina University. Department of Biology.
Format	Theses and dissertations
Production	2004.
Description	xi, 70 leaves : illustrations ; 28 cm
Supplemental Content	Access via ScholarShip
Subjects	Cassia (Genus)--North Carolina. Anti-inflammatory agents. Histamine.

Summary	The present study focused on the potential anti-inflammatory effects of Cassia occidentalis, a plant used medicinally as a treatment for rheumatism and acute inflammation, native to the Old World tropics; however, it is also native to North Carolina. To verify the anti-inflammatory property of C. occidentalis, the release of histamine from MC/9 mast cells by treatment with various extract concentrations was quantitatively measured. Leaves of C. occidentalis were removed and crushed into a powder. This powder underwent a Soxhlet extraction using methylene chloride or absolute methanol. The MC/9 cells were activated to release histamine using Compound 48/80, a mast cell stimulator. Total histamine release was assayed by an Enzyme-Linked Immunosorbent Assay (ELISA). Results indicate that C. occidentalis extracts at 1x104 æg/ml, extracted with methylene chloride, has the ability to inhibit histamine release. Additionally, C. occidentalis extracts at 1x101 æg/ml, extracted with absolute methanol, also inhibits the histamine release from MC/9 cells; however, cells washed once to remove excess extract showed stronger histamine inhibition than cells washed multiple times. Furthermore, C. occidentalis extract, using methylene chloride at 1x104 mcg/ml, has a similar inhibitory effect as the commercially available mast cell stabilizer. Cromolyn, at a therapeutic dose of 2x104 mcg/ml.
General note	Presented to the faculty of the Department of Biology.
General note	Advisor: Gerhard W. Kalmus
Dissertation note	M.S. East Carolina University 2004
Bibliography note	Includes bibliographical references (leaves 66-70).
Genre/form	dissertations.
Genre/form	Academic theses.
Genre/form	Academic theses.
Genre/form	Thèses et écrits académiques.

Determination of optimal concentrations of Cassia occidentalis used to inhibit histamine release from MC/9 mast cells / by Shawn Anthony Moore.

Click here for more information about this title