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003 OCoLC;

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008 070912s2006 xx a bm 000 0 eng d;

035 a| (Sirsi) o170995004;

035 a| (OCoLC)170995004;

049 a| EREE;

050 4 a| QL638.C55b| H63 2006;

100 1 a| Hogan, Katherine Anne,e| author.?| UNAUTHORIZED;

245 14 a| The effect of constant hypoxia on the metabolism of Oreochromis mossambicus /c| by Katherine Anne Hogan.;

264 0 c| 2006.;

300 a| 57 leaves :b| illustrations (some color) ;c| 28 cm;

336 a| textb| txt2| rdacontent;

337 a| unmediatedb| n2| rdamedia;

338 a| volumeb| nc2| rdacarrier;

502 b| M.S.c| East Carolina Universityd| 2006;

500 a| Presented to the faculty of the Department of Biology.;

500 a| Advisor: Mary A. Farwell;

520 3 a| Hypoxia, a reduction in dissolved oxygen, causes stress for aquatic organisms resulting in changes to behavioral, physiological, and biochemical pathways. The objective of this research was to examine how hypoxia affects respiration and energy metabolism of farm-raised Oreochromis mossambicus (Mozambique tilapia). We determined enzyme activities from time zero (t0) to 72 h in fish exposed to 10 % oxygen saturation (~0.8 mg/L). Specifically, we assayed for Na+/K+ ATPase, lactate dehydrogenase, and catalase activity in the gill, liver, and muscle tissue. Na+/K+ ATPase activity in gill did not significantly decrease as hypothesized; however there appears to be a reduction in variance in Na+/K+ ATPase activity the exposed fish over time in all three experiments. Lactate dehydrogenase activity in the muscle did not significantly increase as hypothesized, however Experiment 1 results suggest an increase in activity from to through 12 h, followed by a decrease through 48 h. Catalase activity could only be interpreted in the liver, and there appears to be an increase in activity in Experiment 1 from to through 6 h, followed by a significant decrease through 18 h, then another increase from 18 h to 72 h. Research examining the energy metabolism of Nile tilapia found that oxygen concentrations had to be taken to a near lethal level in order to see a change in metabolic activity (Ishibashi et al. 2002). Our study further showed that 10% oxygen saturation caused little effect on respiration and energy metabolism of Mozambique tilapia, demonstrating their distinction as a hypoxia-tolerant species.;

504 a| Includes bibliographical references (leaves 49-52).;

650 0 a| Mozambique tilapiax| Metabolism.=| ^A909821;

650 0 a| Hypoxia (Water)x| Physiological effect.=| ^A964608;

655 7 a| Academic theses.2| fast0| (OCoLC)fst01726453;

655 7 a| Academic theses.2| lcgft;

655 7 a| Thèses et écrits académiques.2| rvmgf0| (CaQQLa)RVMGF-000001173;

655 2 a| Academic Dissertation.0| (DNLM)D019478?| UNAUTHORIZED;

700 1 a| Farwell, Mary A.,e| degree supervisor.?| UNAUTHORIZED;

710 2 a| East Carolina University.b| Department of Biology.=| ^A637467;

856 41 z| Access via ScholarShipu| http://hdl.handle.net/10342/12501;

949 a| Click on web addressw| asish| joyner101;

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999 a| ASK AT SPECIAL COLLECTIONS DESKw| ASISc| 1i| MQ1936427l| JSCJTHDm| JOYNERr| Ys| Yt| JSCJTHDu| 9/12/2007x| ETDz| JSPECCOLLo| .STAFF. 0;

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The effect of constant hypoxia on the metabolism of Oreochromis mossambicus / by Katherine Anne Hogan.

Author/creator	Hogan, Katherine Anne author.
Other author	Farwell, Mary A., degree supervisor.
Other author	East Carolina University. Department of Biology.
Format	Theses and dissertations
Production	2006.
Description	57 leaves : illustrations (some color) ; 28 cm
Supplemental Content	Access via ScholarShip
Subjects	Mozambique tilapia--Metabolism. Hypoxia (Water)--Physiological effect.

Summary	Hypoxia, a reduction in dissolved oxygen, causes stress for aquatic organisms resulting in changes to behavioral, physiological, and biochemical pathways. The objective of this research was to examine how hypoxia affects respiration and energy metabolism of farm-raised Oreochromis mossambicus (Mozambique tilapia). We determined enzyme activities from time zero (t0) to 72 h in fish exposed to 10 % oxygen saturation (~0.8 mg/L). Specifically, we assayed for Na+/K+ ATPase, lactate dehydrogenase, and catalase activity in the gill, liver, and muscle tissue. Na+/K+ ATPase activity in gill did not significantly decrease as hypothesized; however there appears to be a reduction in variance in Na+/K+ ATPase activity the exposed fish over time in all three experiments. Lactate dehydrogenase activity in the muscle did not significantly increase as hypothesized, however Experiment 1 results suggest an increase in activity from to through 12 h, followed by a decrease through 48 h. Catalase activity could only be interpreted in the liver, and there appears to be an increase in activity in Experiment 1 from to through 6 h, followed by a significant decrease through 18 h, then another increase from 18 h to 72 h. Research examining the energy metabolism of Nile tilapia found that oxygen concentrations had to be taken to a near lethal level in order to see a change in metabolic activity (Ishibashi et al. 2002). Our study further showed that 10% oxygen saturation caused little effect on respiration and energy metabolism of Mozambique tilapia, demonstrating their distinction as a hypoxia-tolerant species.
General note	Presented to the faculty of the Department of Biology.
General note	Advisor: Mary A. Farwell
Dissertation note	M.S. East Carolina University 2006
Bibliography note	Includes bibliographical references (leaves 49-52).
Genre/form	Academic theses.
Genre/form	Academic theses.
Genre/form	Thèses et écrits académiques.

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