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LEADER 03641ctm 2200577 i 4500

001 ocn171148345;

003 OCoLC;

005 20250721152915.0;

008 070913s2006 xx a bm 000 0 eng d;

035 a| (Sirsi) o171148345;

035 a| (OCoLC)171148345;

049 a| EREE;

050 4 a| QK569.B2b| M32 2006;

100 1 a| McClanahan, Ana M.,e| author.?| UNAUTHORIZED;

245 10 a| Detection of endolithic conchocelis in substratum using species-specific PCR primers as probes /c| by Ana M. McClanahan.;

264 0 c| 2006.;

300 a| viii, 78 leaves :b| illustrations (some color) ;c| 28 cm;

336 a| textb| txt2| rdacontent;

337 a| unmediatedb| n2| rdamedia;

338 a| volumeb| nc2| rdacarrier;

502 b| M.S.c| East Carolina Universityd| 2006;

500 a| Presented to the faculty of the Department of Biology.;

500 a| Advisor: John Stiller;

520 3 a| The genus Porphyra C. Agardh is among the most ancient and ubiquitous of the red algae. It is probably the most widely studied seaweed due to ethnobotanical and commercial interests in aquaculture of the edible gametophytic blade. Although the genus has been harvested and eaten for millennia by coastal cultures, little is known about the wild habit, distribution, and life history of Porphyra's filamentous sporophyte, also known as the "conchocelis." This is striking in light of the fact that this endolithic form has persisted over hundreds of millions of years of evolution with very little morphological change. The cryptic habit of the shell-boring filament, and resulting difficulties with locating and identifying species in the field, have prevented any appreciable understanding of the ecology or life history of the conchocelis in the wild. This study develops a method by which sequential PCR can be utilized as an assay for the presence and identification of Porphyra conchocelis at the species level in wild samples of calcareous substrata.;

504 a| Includes bibliographical references (leaves 67-78).;

650 0 a| Porphyrax| Classificationx| Molecular aspects.=| ^A912734;

650 0 a| Porphyrax| Genetics.=| ^A912734;

650 0 a| Polymerase chain reaction.=| ^A254832;

650 0 a| DNA.=| ^A8939;

650 0 a| Aquaculture.=| ^A35286;

650 0 a| Aquacultural engineering.=| ^A367289;

650 7 a| Aquacultural engineering.2| fast0| (OCoLC)fst00811863;

650 7 a| Aquaculture.2| fast0| (OCoLC)fst00811864;

650 7 a| DNA.2| fast0| (OCoLC)fst00886555;

650 7 a| Polymerase chain reaction.2| fast0| (OCoLC)fst01070553;

655 7 a| dissertations.2| aat0| (CStmoGRI)aatgf300028029;

655 7 a| Academic theses.2| fast0| (OCoLC)fst01726453;

655 7 a| Academic theses.2| lcgft;

655 7 a| Thèses et écrits académiques.2| rvmgf0| (CaQQLa)RVMGF-000001173;

700 1 a| Stiller, John W.,e| degree supervisor.?| UNAUTHORIZED;

710 2 a| East Carolina University.b| Department of Biology.=| ^A637467;

856 41 z| Access via ScholarShipu| http://hdl.handle.net/10342/12581;

949 a| Click on web addressw| asish| joyner101;

949 a| Click on web addressw| asish| hsl111;

994 a| C0b| ERE;

596 a| 1 4;

998 a| 1093258;

999 a| ASK AT SPECIAL COLLECTIONS DESKw| ASISc| 1i| MQ1936564l| JSCJTHDm| JOYNERr| Ys| Yt| JSCJTHDu| 9/13/2007x| ETDz| JSPECCOLLo| .STAFF. 0;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 1093258-3001l| JNETm| JOYNERr| Ys| Yt| JNE3ETDu| 5/12/2025x| ETDz| JERESOURCE;

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Detection of endolithic conchocelis in substratum using species-specific PCR primers as probes / by Ana M. McClanahan.

Author/creator	McClanahan, Ana M. author.
Other author	Stiller, John W., degree supervisor.
Other author	East Carolina University. Department of Biology.
Format	Theses and dissertations
Production	2006.
Description	viii, 78 leaves : illustrations (some color) ; 28 cm
Supplemental Content	Access via ScholarShip
Subjects	Porphyra--Classification. --Molecular aspects. Porphyra--Genetics. Polymerase chain reaction. DNA. Aquaculture. Aquacultural engineering.

Summary	The genus Porphyra C. Agardh is among the most ancient and ubiquitous of the red algae. It is probably the most widely studied seaweed due to ethnobotanical and commercial interests in aquaculture of the edible gametophytic blade. Although the genus has been harvested and eaten for millennia by coastal cultures, little is known about the wild habit, distribution, and life history of Porphyra's filamentous sporophyte, also known as the "conchocelis." This is striking in light of the fact that this endolithic form has persisted over hundreds of millions of years of evolution with very little morphological change. The cryptic habit of the shell-boring filament, and resulting difficulties with locating and identifying species in the field, have prevented any appreciable understanding of the ecology or life history of the conchocelis in the wild. This study develops a method by which sequential PCR can be utilized as an assay for the presence and identification of Porphyra conchocelis at the species level in wild samples of calcareous substrata.
General note	Presented to the faculty of the Department of Biology.
General note	Advisor: John Stiller
Dissertation note	M.S. East Carolina University 2006
Bibliography note	Includes bibliographical references (leaves 67-78).
Genre/form	dissertations.
Genre/form	Academic theses.
Genre/form	Academic theses.
Genre/form	Thèses et écrits académiques.

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Detection of endolithic conchocelis in substratum using species-specific PCR primers as probes / by Ana M. McClanahan.

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