Mesenchymal stem cell properties of breast tissue derived cells / by Jennifer Elizabeth Rhinehart.
| Author/creator | Rhinehart, Jennifer Elizabeth author. |
| Other author | Sigounas, George, degree supervisor. |
| Other author | East Carolina University. Department of Biology. |
| Format | Theses and dissertations |
| Production | 2007. |
| Description | viii, 90 leaves : illustrations (some color) ; 28 cm |
| Supplemental Content | Access via ScholarShip |
| Subjects |
| Subject | The isolation and characterization of the bone marrow derived mesenchymal stem cells (MSC) has been well studied. These cells can be isolated through cell surface marker characterization and confirmed through in vitro induction into osteoblasts, adipocytes, and chondrocytes. However, the breast epithelial stem cell hierarchy has been only recently assessed. There are several proposed candidates for the mammary gland stem cell (MaSC)/progenitors expressing certain surface antigens and having the ability to form branching 3D structures. This data supports the hypothesis that breast tissue-derived primary cells have mesenchymal stem cell properties and characteristics similar to those found in human bone marrow. It is shown for first time that both early (EPCs) and late (LPCs) passage human mammary cells express antigens similar to bone marrow MSC and mammary stem cells including: CD73, CD44v6, CD29, CD90, CD49f, MUC-1, CK19, and CK14. Mammary cells can be induced to differentiate into various cell lineages such as adipocytes, myocytes, osteocytes and epithelial cells -properties commonly shared by MSCs. However, there are several morphological, physiological, biochemical and functional differences between EPCs and LPCs Including clonality, levels/types of antigens expressed, cycling, and organogenesis. Based on this study, we propose a new breast stem cells hierarchy in which: 1) EPCs are more primitive than LPCs; 2) EPCs and LPCs are termed early (EMaSC) and (LMaSC) mammary gland stem cell, respectively; and 3) EPCs and LPCs may be progenitors for the mammary mesenchymal stem cell (MaMSC). |
| General note | Presented to the faculty of the Department of Biology. |
| General note | Advisor: George Sigounas |
| Dissertation note | M.S. East Carolina University 2007 |
| Bibliography note | Includes bibliographical references (leaves 84-90). |
| Genre/form | Academic theses. |
| Genre/form | Academic theses. |
| Genre/form | Thèses et écrits académiques. |