A comparison of three methods of gene delivery into channel catfish (Ictalurus punctatus) / by Ben N. Trimpi.
| Author/creator | Trimpi, Ben N. author. |
| Other author | McConnell, Thomas J., degree supervisor. |
| Other author | East Carolina University. Department of Biology. |
| Format | Theses and dissertations |
| Production | 2007. |
| Description | 48 leaves : illustrations (some color) ; 29 cm |
| Supplemental Content | Access via ScholarShip |
| Subjects |
| Summary | The ability to introduce DNA into a eukaryotic cell, termed transfection, has brought forth a great deal of research utilizing its use. Basic science understandings, transgenic animal production, human and animal vaccination processes have, and continue to benefit from transfection technologies. Certain fish species have become common model organisms. This, in large part, is due to the economical contribution and evolutionary perspective associated with fish species. Information involving transfection technologies in fish is limited. The purpose of this study was to evaluate electroporation, calcium phosphate co-precipitation, and lipofection in G5F fibroblasts derived from channel catfish. The toxicity and gene delivery efficiency for each method was determined. This data was used to compare the three methods in order to delineate the best-fit method. Electroporation yielded the highest transfection efficiency. Lipofection was a more efficient method of gene delivery when compared to calcium phosphate co precipitation, but cell viabilities were low. This study will aid in future endeavors involving the use of transfection technologies in channel catfish, Ictalurus punctatus. |
| General note | Presented to the faculty of the Department of Biology. |
| General note | Advisor: Thomas J. McConnell |
| Dissertation note | M.S. East Carolina University 2007 |
| Bibliography note | Includes bibliographical references (leaves 44-48). |
| Genre/form | dissertations. |
| Genre/form | Academic theses. |
| Genre/form | Academic theses. |
| Genre/form | Thèses et écrits académiques. |