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003 OCoLC;

005 20241021155018.0;

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035 a| (Sirsi) o213466180;

035 a| (OCoLC)213466180;

049 a| EREE;

050 4 a| RC280.P7b| H83 2007;

100 1 a| Hudgins, Ryan Majette,e| author.?| UNAUTHORIZED;

245 14 a| The effect of erythropoietin on prostate cancer cells expressing the erythropoietin receptor /c| by Ryan Majette Hudgins.;

264 0 c| 2007.;

300 a| 90 leaves :b| illustrations (some color) ;c| 29 cm;

336 a| textb| txt2| rdacontent;

337 a| unmediatedb| n2| rdamedia;

338 a| volumeb| nc2| rdacarrier;

502 b| M.S.c| East Carolina Universityd| 2007;

500 a| Presented to the faculty of the Department of Biology.;

500 a| Advisor: George Sigounas;

520 3 a| Previous studies have reported that erythropoietin (EPO) is a pleiotropie hormone with multiple biological properties. Furthermore, it has been recently reported that various neoplastic tissues and human cancer cell lines express EPO and/or the EPO receptor (EPOR), suggesting the possible existence of an autocrine-paracrine EPO-EPOR system. These studies encouraged us to further investigate the functional consequences of a potential EPO effect on 3 human prostate cancer cell lines: DU-145, PC-3 and LNCaP. All lines express the EPOR, but the PC-3 and LNCaP cells additionally express EPO. We assessed the effect of EPO on cell proliferation and the protective effect of EPO on apoptosis, cytotoxicity, and DNA fragmentation after irradiation. We found that EPO induced an 8% increase in proliferation of DU-145 cells and had a marginal effect on the growth of PC-3 cells and LNCaP cells (p>0.05). When EPO treatment was combined with radiation, apoptosis and cytotoxicity of PC-3 and DU-145 cells was not affected. EPO synergized with radiation and increased the number of single strand DNA breaks in DU-145 and PC-3 cells up to 3-fold compared to control groups. The treatment schedule and cell type seemed to play a critical role in radiation-induced DNA damage. When EPO was combined with irradiation treatment, the antioxidant capacity of the cells was highly (up to 100%) suppressed. We concluded that EPO may modulate the growth and alter the chemoradiosensitivity of EPOR-expressing prostate cancer cells ex vivo.;

504 a| Includes bibliographical references (leaves 85-90).;

650 0 a| Prostatex| Cancerx| Research.=| ^A1195499;

650 0 a| Cancerx| Research.=| ^A4333;

650 0 a| Erythropoietin.=| ^A331869;

650 0 a| Glycoprotein hormones.=| ^A651211;

650 2 a| Erythropoietin.0| (DNLM)D004921=| ^A1473681;

655 2 a| Academic Dissertation.0| (DNLM)D019478?| UNAUTHORIZED;

650 7 a| Cancerx| Research.2| fast0| (OCoLC)fst00845497;

650 7 a| Erythropoietin.2| fast0| (OCoLC)fst00915096;

650 7 a| Glycoprotein hormones.2| fast0| (OCoLC)fst00943790;

655 7 a| dissertations.2| aat0| (CStmoGRI)aatgf300028029;

655 7 a| Academic theses.2| fast0| (OCoLC)fst01726453;

655 7 a| Academic theses.2| lcgft;

655 7 a| Thèses et écrits académiques.2| rvmgf0| (CaQQLa)RVMGF-000001173;

700 1 a| Sigounas, George,e| degree supervisor.?| UNAUTHORIZED;

710 2 a| East Carolina University.b| Department of Biology.=| ^A637467;

856 41 z| Access via ScholarShipu| http://hdl.handle.net/10342/12518;

949 a| Click on web addressw| asish| joyner101;

949 a| Click on web addressw| asish| hsl111;

994 a| C0b| ERE;

596 a| 1 4;

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999 a| ASK AT SPECIAL COLLECTIONS DESKw| ASISc| 1i| MQ2421463l| JSCJTHDm| JOYNERr| Ys| Yt| JSCJTHDu| 3/19/2008x| ETDz| JSPECCOLLo| .STAFF. 0;

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The effect of erythropoietin on prostate cancer cells expressing the erythropoietin receptor / by Ryan Majette Hudgins.

Author/creator	Hudgins, Ryan Majette author.
Other author	Sigounas, George, degree supervisor.
Other author	East Carolina University. Department of Biology.
Format	Theses and dissertations
Production	2007.
Description	90 leaves : illustrations (some color) ; 29 cm
Supplemental Content	Access via ScholarShip
Subjects	Prostate--Cancer. --Research. Cancer--Research. Erythropoietin. Glycoprotein hormones. Erythropoietin.

Summary	Previous studies have reported that erythropoietin (EPO) is a pleiotropie hormone with multiple biological properties. Furthermore, it has been recently reported that various neoplastic tissues and human cancer cell lines express EPO and/or the EPO receptor (EPOR), suggesting the possible existence of an autocrine-paracrine EPO-EPOR system. These studies encouraged us to further investigate the functional consequences of a potential EPO effect on 3 human prostate cancer cell lines: DU-145, PC-3 and LNCaP. All lines express the EPOR, but the PC-3 and LNCaP cells additionally express EPO. We assessed the effect of EPO on cell proliferation and the protective effect of EPO on apoptosis, cytotoxicity, and DNA fragmentation after irradiation. We found that EPO induced an 8% increase in proliferation of DU-145 cells and had a marginal effect on the growth of PC-3 cells and LNCaP cells (p>0.05). When EPO treatment was combined with radiation, apoptosis and cytotoxicity of PC-3 and DU-145 cells was not affected. EPO synergized with radiation and increased the number of single strand DNA breaks in DU-145 and PC-3 cells up to 3-fold compared to control groups. The treatment schedule and cell type seemed to play a critical role in radiation-induced DNA damage. When EPO was combined with irradiation treatment, the antioxidant capacity of the cells was highly (up to 100%) suppressed. We concluded that EPO may modulate the growth and alter the chemoradiosensitivity of EPOR-expressing prostate cancer cells ex vivo.
General note	Presented to the faculty of the Department of Biology.
General note	Advisor: George Sigounas
Dissertation note	M.S. East Carolina University 2007
Bibliography note	Includes bibliographical references (leaves 85-90).
Genre/form	dissertations.
Genre/form	Academic theses.
Genre/form	Academic theses.
Genre/form	Thèses et écrits académiques.

The effect of erythropoietin on prostate cancer cells expressing the erythropoietin receptor / by Ryan Majette Hudgins.

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