Therapeutic proteins : methods and protocols / edited by C. Mark Smales and David C. James.
| Other author | Smales, C. Mark. |
| Other author | James, David C. (David Cameron), 1963- |
| Format | Book |
| Publication Info | Totowa, N.J. : Humana Press, ©2005. |
| Description | xiv, 482 pages : illustrations ; 24 cm. |
| Subjects |
| Series | Methods in molecular biology ; 308 Methods in molecular biology (Clifton, N.J.) v. 308. ^A682607 |
| Contents | Biopharmaceutical proteins / John R. Birch and Yemi Onakunle -- Expression of antibody fragments by periplasmic secretion in Escherichia coli / Andrew G. Popplewell ... [et al.] -- Secretory production of therapeutic proteins in Escherichia coli / Sang Yup Lee, Jong Hyun Choi, and Sang Jun Lee -- Expression of recombinant LTB protein in marine vibrio VSP60 / Wang Yili and Si Lusheng -- Heterologous gene expression in yeast / Lee J. Byrne, Kenneth J. O'Callaghan, and Mick F. Tuite -- Pharmaceutical proteins from methylotrophic yeasts / Eric C. de Bruin ... [et al.] -- Expression of human papillomavirus type 16 L1 in baculovirus expression systems / Zheng Jin, Si Lusheng, and Wang Yili -- Large-scale transient expression of therapeutic proteins in mammalian cells / Sabine Geisse, Martin Jordan, and Florian M. Wurm -- Site-specific integration for high-level protein production in mammalian cells / Bhaskar Thyagarajan and Michele P. Calos -- Production of recombinant therapeutic proteins by mammalian cells in suspension culture / Lily Chu, Ilse Blumentals, and Gargi Maheshwari -- Transgene control engineering in mammalian cells / Beat P. Kramer and Martin Fussenegger -- Fusion to albumin as a means to slow the clearance of small therapeutic proteins / William P. Sheffield, Teresa R. McCurdy, and Varsha Bhakta -- High throughput recovery of therapeutic proteins from the inclusion bodies of Escherichia coli / Amulya K. Panda -- The isolation, solubilization, refolding, and chromatographic purification of recombinant human growth hormone from inclusion bodies of Escherichia coli cells / Amulya K. Panda, A. N. S. Eshwari, and Lalit C. Garg -- Large-scale preparation of factor VIIa from human plasma / Teruhisa Nakashima and Kazuhiko Tomokiyo -- Purification of clinical-grade monoclonal antibodies by chromatographic methods / Alberto L. Horenstein, Ilaria Durelli, and Fabio Malavasi -- Virus elimination and validation / Nicola Boschetti and Anna Johnston -- Virus removal by nanofiltration / Marina Korneyeva and Scott Rosenthal |
| Contents | Determining residual host cell antigen levels in purified recombinant proteins by slot blot and scanning laser densitometry / Aaron P. Miles, Daming Zhu, and Allan Saul -- Principles of biopharmaceutical protein formulation / Scott P. Sellers and Yuh-Fun Maa -- Solid-state protein formulation / Yuh-Fun Maa and Scott P. Sellers -- Stabilization of therapeutic proteins by chemical and physical methods / Ken-ichi Izutsu -- Extraction and characterization of vaccine antigens from water-in-oil adjuvant formulations / Aaron P. Miles and Allan Saul -- Probing reversible self-association of therapeutic proteins by sedimentation velocity in the analytical ultracentrifuge / Bernardo Perez-Ramirez and John J. Steckert -- Biological characterization of pegylated interferons / Lei Xie ... [et al.] -- Characterization of interferon alpha-2B pegylated via carboxyalkylation / David C. Wylie ... [et al.] -- Quantifying recombinant proteins and their degradation products using SDS-PAGE and scanning laser densitometry / Aaron P. Miles and Allan Saul. |
| Bibliography note | Includes bibliographical references and index. |
| LCCN | 2004021242 |
| ISBN | 1588293904 : |
Availability
| Library | Location | Call Number | Status | Item Actions |
|---|---|---|---|---|
| Laupus | Books - Stacks | QU 55 T397 2005 | ✔ Available | Place Hold |