Effects of malachite green on zoospores of the marine fungus Lagenidium calinectes Couch / by Michael T. Hall.
| Author/creator | Hall, Michael T. author. |
| Other author | Bland, Charles E., degree supervisor. |
| Other author | East Carolina University. Department of Biology. |
| Format | Theses and dissertations |
| Production | 1978. |
| Description | [vii], 81 leaves : illustrations, plates ; 28 cm |
| Supplemental Content | Access via ScholarShip |
| Subjects |
| Summary | The fungicide, malachite green, has been found effective in inhibiting growth of the marine fungus, Lagenidium callinectes Couch at a concentration (0.015 ppm) which makes it of potential use in controlling this and other fungi-causing diseases of cultured marine Crustacea. In spite of the use of malachite green in this and related areas, little is known of its site and mode of action as a fungicide. In an effort to gain information concerning both these factors, electron microscope observations were made of zoospores of L. callinectes which had been treated with malachite green for varying time intervals. When treated spores were compared with untreated, marked differences were noted in mitochondrial fine structure. A progressive degeneration of the mitochondria was observed in samples taken at 10-minute intervals for one hour. Mitochondria of 60-minute treated spores were swollen, spherical in shape, dense, had fragmented cristae and clumped matrices. Mitochondria of controls were elongate, had regular cristae, and a granular matrix. Other cell organelles appeared unaffected by treatment. Correspondingly, sublethal concentrations of malachite green were found to inhibit 0₂ uptake by zoospores of L. callinectes in a manner like 2,4-dinitrophenol. Thus, the site of action of malachite green is probably the mitochondrion where it possibly acts by uncoupling respiratory oxidative processes from phosphorylation. |
| Local note | Joyner-"Presented to the faculty of the Department of Biology ... in partial fulfillment of the requirements for the degree Master of Science in Biology." |
| General note | Submitted to the faculty of the Department of Biology. |
| General note | Advisor: Charles E. Bland |
| Dissertation note | M.S. East Carolina University 1978 |
| Bibliography note | Includes bibliographical references (leaves 77-81). |
| Genre/form | Academic theses. |
| Genre/form | Academic theses. |
| Genre/form | Thèses et écrits académiques. |
Availability
| Library | Location | Call Number | Status | Item Actions |
|---|---|---|---|---|
| Joyner | University Archives | ASK AT SPECIAL COLLECTIONS DESK | ✔ Available | Request Material |
| Electronic Resources | Access Content Online | ✔ Available |