Prolonged administration of Diethylstilbestrol (des) and subsequent fecundity / by George E. Glosson.

Author/creator Glosson, George E. author.
Other author Simpson, Everett C., degree supervisor.
Other author East Carolina University. Department of Biology.
Format Theses and dissertations
Production1976.
Description36 leaves, 17 leaves of plates : illustrations ; 29 cm
Supplemental ContentAccess via ScholarShip
Subjects

Summary A total of 120 albino rats (Sprague-Dawley strain) were injected subcutaneously with 1.25 and 2.5 mg. DES for five, ten and fifteen estrous cycles at late estrus. Appropriate controls were injected with the oil vehicle. After completing treatment, the rats were bred and the incidence of pregnancy was determined by microscopic examination of daily vaginal lavages. Pregnant animals were sacrificed on day 15. There was a significant reduction in the number of normal fetuses as the number of injections increased. This reduction in the number of normal fetuses continued to zero fertility in the fifteen cycle sequence groups. No products of conception were found in the DES-treated animals showing infections of the reproductive tract. Compared to the controls, there was a marked increase in uterine infections in all DES-treated groups except for the 1.25 mg. DES five cycle group. Examination of the reproductive tract revealed one uterine and five ovarian infections in the five cycle DES-treated groups. Of these infections, the uterine and two ovarian infections were seen in the high dosage level group. In the ten cycle DES-treated groups, there were two uterine infections and three ovarian infections observed at both dosage level groups. Two and three uterine infections were observed in the fifteen cycle low and high dosage level groups, respectively. There were three and six ovarian infections observed in the fifteen cycle low and high dosage level groups, respectively. There was a significant difference between the controls and their respective treated groups in the percent animals pregnant at day 15. There was a significant difference in percent of animals pregnant between dosage levels except between the ten cycle DES-treated groups. Animals not gravid at 15 days were allowed to breed as a follow-up of fertility; however, no products of conception were ever observed in these DES-treated animals.
Local noteJoyner-"Presented to the faculty of the Department of Biology ... in partial fulfillment of the requirements for the degree Master of Science in Biology."
General notePresented to the faculty of the Department of Biology.
General noteAdvisor: Everett C. Simpson
Dissertation noteM.S. East Carolina University 1976
Bibliography noteIncludes bibliographical references (leaves 34-36).
Genre/formdissertations.
Genre/formAcademic theses.
Genre/formAcademic theses.
Genre/formThèses et écrits académiques.

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