Microbial metabolism phthalic acid isomers / by Eunhee Shim.

Author/creator Shim, Eunhee author.
Other author Ito, Takeru, degree supervisor.
Other author East Carolina University. Department of Biology.
Format Theses and dissertations
Production1975.
Description51 leaves : illustrations ; 28 cm
Supplemental ContentAccess via ScholarShip
Subjects

Summary Three soil microorganisms (probably Pseudomonas), were isolated by enrichment culture technique, using either phthalic acid or isophthalic acid, or terephthalic acid, as a sole source of carbon. Oxidative metabolism of three organisms was studied by determination of oxygen consumption of the organisms with various suspected metabolic intermediates using an ox)'gen electrode and by determination of spectra of intermediates using a spectrophotometer. The following results were obtained from such studies. Terephthalic acid-grown cells converted terephthalic acid into protocatecliuic acid, and isophthalic acid-grown cells oxidized isophthalic acid to the same product. They oxidized protocatechuic acid to beta-carboxycis,cis-muconic acid. None of the tested compounds suspected to be the intermediates of phthalic acid degradation including protocatechuic acid were oxidized by phthalic acid-grown cells. A new, possible intimidate was postulated, which was 2,7-diketo-3,cis-5,cis-octadienoic-l,8-dicarboxylic acid.
Local noteJoyner-"Presented to the faculty of the Department of Biology ... in partial fulfillment of the requirements for the degree Master of Arts in Biology."
General notePresented to the Faculty of the Department of Biology.
General noteAdvisor: Takeru Ito
Dissertation noteM.A. East Carolina University 1975
Bibliography noteIncludes bibliographical references (leaves 48-51).
Genre/formAcademic theses.
Genre/formAcademic theses.
Genre/formThèses et écrits académiques.

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