The effects of selected fungicides on the growth and development of Lagenidium callinectes couch / by Donald Gene Ruch.

Author/creator Ruch, Donald Gene, 1948- author.
Other author Bland, Charles E., degree supervisor.
Other author East Carolina University. Department of Biology.
Format Theses and dissertations
Production1974.
Description122 leaves : illustrations ; 28 cm
Supplemental ContentAccess via ScholarShip
Subjects

Summary The effects of twelve potentially fungitoxic compounds on growth and development of two strains of Lagenidium callinectes were studied. The compounds tested were Benlate, Captan, Dichlone, Difolatan, DS 9073, Dyrene, malachite green, Manzate 200 (Dithane M-45), Terraclor, Treflan, tribasic copper sulfate, and Vitavax. In vitro tests included determination of the minimal lethal concentration (LC₁₀₀) of each fungicide, the effects of the LC₁₀₀ on length of motility, flagella retraction or detachment, encystment and germination, and the length of time necessary for the LC₁₀₀ of each fungicide to kill zoospores of callinectes. Various animal toxicity tests were also conducted. In evaluating the total efficacy of these twelve compounds, only two, malachite green and DS 9073, proved to be of potential use as disease deterrents in mariculture. The problems associated with both of these compounds are discussed. The effects of exposures of 10-60 min. in the LC₁₀₀ of Captan on the ultrastructure of the spores of I., callinectes were studied also. Ultrastructural changes caused by exposure to Captan included complete disappearance of the mitochondrial matrix and disappearance of most of the cristae, clumping of the chromatin and "washing out" of the nuclear matrix, swelling and wrinkling of the nuclear envelope, extreme swelling of the cisternae of the endoplasmic reticulum. apparent dumping of the free ribosomes within the cytoplasm of the spore, and ultimately, breakdown of the plasma membrane.
Local noteJoyner-"Presented to the faculty of the Department of Biology ... in partial fulfillment for the degree Master of Arts in Biology."
General notePresented to the Faculty of the Department of Biology
General noteAdvisor: Charles E. Bland
Dissertation noteM.A. East Carolina University 1974
Bibliography noteIncludes bibliographical references (leaves 110-122).
Genre/formdissertations.
Genre/formAcademic theses.
Genre/formAcademic theses.
Genre/formThèses et écrits académiques.

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