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100 1 a| Sloan, Ruben Carnell.?| UNAUTHORIZED;

245 10 a| Effect of duodenal-jejunal bypass on skeletal muscle insulin signaling in Goto-Kakizaki rats /c| by Ruben Carnell Sloan III.;

260 a| [Greenville, N.C.] :b| East Carolina University,c| 2009.;

300 a| 50 pages :b| illustrations (some color), digital, PDF file;

336 a| text2| rdacontent;

337 a| computer2| rdamedia;

338 a| online resource2| rdacarrier;

500 a| Presented to the faculty of the Department of Exercise and Sport Science.;

500 a| Advisor: Timothy P. Gavin.;

500 a| Title from PDF t.p. (viewed May 25, 2010).;

502 b| M.S.c| East Carolina Universityd| 2009.;

504 a| Includes bibliographical references.;

520 3 a| Gastric bypass surgery (RYGBP) for the treatment of obesity has proven to clinically reverse type 2 diabetes mellitus. RYGBP involves both gastric reduction and bypass of the proximal small intestine. Duodenal-Jejunal Bypass (DJB) is a surgical procedure that bypasses the proximal small intestine without gastric reduction and has been shown to improve oral glucose tolerance in Goto-Kakizaki (GK) rats, a non-obese animal model of T2DM. We hypothesized that DJB may improve oral glucose tolerance in GK rats by improving insulin signaling in skeletal muscle, the main depot for insulin stimulated glucose uptake. DJB was performed on male 10-12 week old GK rats (GK-DJB), and sham operations were performed on GK rats (GK-Sham). Insulin stimulated IRS-1, phospho-serine 307 of IRS-1, Akt, and phospho-Akt were determined using Western blot. Phospho-Akt was significantly higher in GK-DJB when compared to GK-Sham in soleus and tended to be higher in gastrocnemius (p=0.107). Akt was significantly higher in GK-DJB when compared to GK-Sham in gastrocnemius and tended to be higher in soleus (p=0.074). Phospho-serine 307 of IRS-1 and total IRS-1 were not different between GK-DJB and GK-Sham in gastrocnemius. In conclusion, bypassing the proximal small intestine (DJB) improves components of skeletal muscle insulin signaling in GK rats, providing mechanistic evidence for the improvement in oral glucose tolerance associated with DJB.;

538 a| System requirements: Adobe Reader.;

538 a| Mode of access: World Wide Web.;

650 0 a| Diabetesx| Research.=| ^A125962;

650 0 a| Insulin.=| ^A3807;

650 0 a| Gastric bypass.=| ^A879542;

650 0 a| Duodenumx| Surgery.=| ^A1017282;

650 0 a| Jejunumx| Surgery.=| ^A1017302;

650 0 a| Rats as laboratory animals.=| ^A10876;

653 a| Biology, Physiology;

653 a| Health Sciences, Medicine and Surgery;

653 a| Biology, Molecular;

700 1 a| Gavin, Timothy P.?| UNAUTHORIZED;

710 2 a| East Carolina University.b| Department of Exercise and Sport Science.?| UNAUTHORIZED;

856 40 z| Access via ScholarShipu| http://hdl.handle.net/10342/1893;

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999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 2150751-2001l| JNETm| JOYNERr| Ys| Yt| JNE3ETDu| 12/8/2009x| ETDz| JERESOURCE;

Effect of duodenal-jejunal bypass on skeletal muscle insulin signaling in Goto-Kakizaki rats / by Ruben Carnell Sloan III.

Author/creator	Sloan, Ruben Carnell
Other author	Gavin, Timothy P.
Other author	East Carolina University. Department of Exercise and Sport Science.
Format	Theses and dissertations
Publication Info	[Greenville, N.C.] : East Carolina University, 2009.
Description	50 pages : illustrations (some color), digital, PDF file
Supplemental Content	Access via ScholarShip
Subjects	Diabetes--Research. Insulin. Gastric bypass. Duodenum--Surgery. Jejunum--Surgery. Rats as laboratory animals.

Summary	Gastric bypass surgery (RYGBP) for the treatment of obesity has proven to clinically reverse type 2 diabetes mellitus. RYGBP involves both gastric reduction and bypass of the proximal small intestine. Duodenal-Jejunal Bypass (DJB) is a surgical procedure that bypasses the proximal small intestine without gastric reduction and has been shown to improve oral glucose tolerance in Goto-Kakizaki (GK) rats, a non-obese animal model of T2DM. We hypothesized that DJB may improve oral glucose tolerance in GK rats by improving insulin signaling in skeletal muscle, the main depot for insulin stimulated glucose uptake. DJB was performed on male 10-12 week old GK rats (GK-DJB), and sham operations were performed on GK rats (GK-Sham). Insulin stimulated IRS-1, phospho-serine 307 of IRS-1, Akt, and phospho-Akt were determined using Western blot. Phospho-Akt was significantly higher in GK-DJB when compared to GK-Sham in soleus and tended to be higher in gastrocnemius (p=0.107). Akt was significantly higher in GK-DJB when compared to GK-Sham in gastrocnemius and tended to be higher in soleus (p=0.074). Phospho-serine 307 of IRS-1 and total IRS-1 were not different between GK-DJB and GK-Sham in gastrocnemius. In conclusion, bypassing the proximal small intestine (DJB) improves components of skeletal muscle insulin signaling in GK rats, providing mechanistic evidence for the improvement in oral glucose tolerance associated with DJB.
General note	Presented to the faculty of the Department of Exercise and Sport Science.
General note	Advisor: Timothy P. Gavin.
General note	Title from PDF t.p. (viewed May 25, 2010).
Dissertation note	M.S. East Carolina University 2009.
Bibliography note	Includes bibliographical references.
Technical details	System requirements: Adobe Reader.
Technical details	Mode of access: World Wide Web.

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Effect of duodenal-jejunal bypass on skeletal muscle insulin signaling in Goto-Kakizaki rats / by Ruben Carnell Sloan III.

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