Characterization and differentiation of NALM-6 and Jurkat cell lines using confocal laser scanning microscropy [sic] / by Rodney Chason Hill.
| Author/creator | Hill, Rodney Chason author. |
| Other author | McConnell, Thomas J., degree supervisor. |
| Other author | East Carolina University. Department of Biology. |
| Format | Theses and dissertations |
| Production | 2009. |
| Description | 80 leaves : color illustrations ; 28 cm |
| Supplemental Content | Access via ScholarShip |
| Subjects |
| Summary | Confocal microscopy is an optical imaging technique that gives the ability to examine a single biological cell in three-dimensions by constructing image "slices" of the different layers of the cell. It allows users to examine different aspects of cellular morphology and processes, and has extreme research potential for biological application. Laser scanning confocal microscopy has proved to be most suitable for the analysis of structural details of thick specimens, and promises to be of great potential in providing 3-D volume renderings of living cells or tissues (Singh 1998). Leukemia is cancer of blood forming tissues, characterized by overcrowding of healthy blood cells by abnormal, cancerous blood cells. Diagnosis of leukemia usually consists of pathologic examination of bone marrow or blood, cytogenetics, and/or molecular analysis of DNA/RNA. While confocal microscopy has been applied to study some aspects of cancerous cells, it has not been used to differentiate between types of cancerous cells. This study focuses on applying confocal microscopy as a differentiative tool for comparing leukemic and nonleukemic cell lines. By fluorescently staining the nuclei and mitochondria of the cells of interest prior to imaging, these areas in the cell are visible during imaging. It was anticipated that with the nuclear and mitochondrial staining, morphological differences would be found between different acute lymphoid leukemia (ALL) cell lines, ALL patient blood cells, and non-leukemic donor blood cells. Acute myeloid leukemia (AML) cell lines and AML patient blood samples were also imaged for further examination. The results of this study suggest that confocal microscopy is capable of detecting morphological differences between different types of leukemic cells. |
| General note | Presented to the faculty of the Department of Biology. |
| General note | Advisor: Thomas J. McConnell |
| Dissertation note | M.S. East Carolina University 2009 |
| Bibliography note | Includes bibliographical references (leaves 45-48). |
| Genre/form | Academic theses. |
| Genre/form | Academic theses. |
| Genre/form | Thèses et écrits académiques. |
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