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LEADER 05085cam 2200685 a 4500

001 ocn636917507;

003 OCoLC;

005 20141212053226.0;

008 100526s2010 nyua b 001 0 eng ;

015 a| GBB0680002| bnb;

016 7 a| 0155670842| Uk;

020 a| 9781607617686 (hbk.);

020 a| 1607617684 (hbk.);

020 a| 9781607617693 (e-ISBN);

020 a| 1607617692 (e-ISBN);

029 1 a| AU@b| 000046084538;

035 a| (Sirsi) o636917507;

035 a| (OCoLC)636917507;

049 a| NEHH;

060 4 a| QU 58.7b| R6277 2010;

070 0 a| QH450b| .R58 2010;

082 04 a| 572.8/652| 22;

090 a| QH450.25b| .R63 2010;

245 00 a| RNAi and microRNA-mediated gene regulation in stem cells :b| methods, protocols, and applications /c| edited by Baohong Zhang and Edmund J. Stellwag.;

300 a| xi, 261 pages :b| illustrations (some color) ;c| 27 cm.;

336 a| text2| rdacontent;

337 a| unmediated2| rdamedia;

338 a| volume2| rdacarrier;

490 1 a| Methods in molecular biology,x| 1064-3745 ;v| 650;

490 1 a| Springer protocols;

504 a| Includes bibliographical references and index.;

505 0 a| RNAi in stem cells : current status and future perspectives / Gang-Ming Zou -- A brief introduction to RNAi and microRNAs in stem cells / Alexander K. Murashov -- Forward RNAi screens in human stem cells / Christine Karlsson, Jonas Larsson, and Aurélie Baudet -- A recessive genetic screen for components of the RNA interference pathway in mouse embryonic stem cells / Melanie I Trombly and Xiaozhong Wang -- Construction and application of random dsRNA interference library for functional genetic screens in embryonic stem cells / Xiaoxing Cheng and Rui Jian -- Establishing efficient siRNA knockdown in stem cells using fluorescent oligonucleotides / Stephen W. Chen and Steve K.W. Oh -- Loss-of-function studies in mouse embryonic stem cells using the pHYPER shRNA plasmic vector / Soizik Berlivet, Martin Houlard, and Matthieu Gérard -- Regulation and/or repression of cholinergic differentiation of murine embryonic stem cells using RNAi directed against transcription factor L3/Lhx8 / Takayuki Manabe and Akio Wanaka -- Silencing of rho-GDI[gamma] by RNAi promotes the differentiation of neural stem cells / Jiao Wang, Wei Lu, and Tieqiao Wen -- RNAi knockdown of redox signaling protein Ape1 in the differentiation of mouse embryonic stem cells / Gang-Ming Zou, Cynthia LeBron, and Yumei Fu -- Proteins involved in cell migration from glioblastoma neurospheres analyzed by overexpression and siRNA-mediated knock-down / Carsten Hagemann ... [et al.] -- An efficient transfection method for mouse embryonic stem cells / Jun-Yang Liou, Bor-Sheng Ko, and Tzu-Ching Chang -- Semi-quantitative analysis of transient single-cell gene expression in embryonic stem cells by femtoinjection / Mikako Saito and Hideaki Matsuoka -- Preparation and analysis of microRNA libraries using the Illumiina massively parallel sequencing technology / Ryan D. Morin ... [et al.] -- Assessing in vivo microRNA function in the germline stem cells of the Drosophila ovary / Kin Chan and Hannele Ruohola-Baker -- Monitoring microRNA expression during embryonic stem-cell differentiation using quantitative real-time PCR (qRT-PCR) / Xiaoping Pan ... [et al.] -- Engineering human mesenchymal stem cells to release adenosine using miRNA technology / Gaoying Ren and Detlev Boison -- Efficient gene knockdowns in mouse embryonic stem cells using microRNA-based shRNAs / Jianlong Wang.;

650 0 a| Genetic regulation.=| ^A6251;

650 0 a| Stem cells.=| ^A390101;

650 12 a| MicroRNAsv| Laboratory Manuals.=| ^A1032967;

650 12 a| RNA Interferencev| Laboratory Manuals.=| ^A1018069;

650 12 a| Stem Cellsv| Laboratory Manuals.=| ^A930050;

650 22 a| Gene Expression Regulation.=| ^A920642;

650 22 a| RNAx| analysis.=| ^A922134;

700 1 a| Zhang, Baohonge| editor.=| ^A1031945;

700 1 a| Stellwag, Edmund J.q| (Edmund Johannes),d| 1951-e| editor.=| ^A1031239;

776 1 t| RNAi and microRNA-mediated gene regulation in stem cellsw| (OCoLC)658085849;

830 0 a| Methods in molecular biology (Clifton, N.J.)v| v. 650.=| ^A682607;

830 0 a| Springer protocols (Series)=| ^A997552;

949 a| QU 58.7 R627 2010h| HSL70i| 31740082683164o| hdjr;

994 a| C0b| NEH;

938 a| YBP Library Servicesb| YANKn| 3325198;

938 a| Blackwell Book Serviceb| BBUSn| R1864360;

590 a| Laupus-WCP;

590 a| ECU author;

096 a| QU 58.7 R627 2010;

596 a| 1 4;

998 a| 2318601;

999 a| QH450.25 .R63 2010w| LCc| 1i| 30372014738725d| 4/18/2013e| 4/16/2013f| 2/13/2012g| 1l| JGESm| JOYNERr| Ys| Yt| JGESBKu| 11/5/2010x| BOOKz| JSTACKS;

999 a| QU 58.7 R627 2010w| NLMc| 1i| 31740082683164d| 3/17/2025e| 2/3/2025f| 1/28/2011g| 1l| HSLBKm| HSLn| 1p| $139.00r| Ys| Yt| HBKu| 11/19/2010x| BOOKz| HSTACKSo| .STAFF. hdjr;

RNAi and microRNA-mediated gene regulation in stem cells : methods, protocols, and applications / edited by Baohong Zhang and Edmund J. Stellwag.

Other author	Zhang, Baohong editor.
Other author	Stellwag, Edmund J. (Edmund Johannes), 1951- editor.
Format	Book
Publication Info	New York : Humana Press, ©2010.
Description	xi, 261 pages : illustrations (some color) ; 27 cm.
Subjects	Genetic regulation. Stem cells. MicroRNAs--Laboratory Manuals. RNA Interference--Laboratory Manuals. Stem Cells--Laboratory Manuals. Gene Expression Regulation. RNA--analysis.

Series	Methods in molecular biology, 1064-3745 ; 650 Springer protocols Methods in molecular biology (Clifton, N.J.) v. 650. ^A682607 Springer protocols (Series) ^A997552
Contents	RNAi in stem cells : current status and future perspectives / Gang-Ming Zou -- A brief introduction to RNAi and microRNAs in stem cells / Alexander K. Murashov -- Forward RNAi screens in human stem cells / Christine Karlsson, Jonas Larsson, and Aurélie Baudet -- A recessive genetic screen for components of the RNA interference pathway in mouse embryonic stem cells / Melanie I Trombly and Xiaozhong Wang -- Construction and application of random dsRNA interference library for functional genetic screens in embryonic stem cells / Xiaoxing Cheng and Rui Jian -- Establishing efficient siRNA knockdown in stem cells using fluorescent oligonucleotides / Stephen W. Chen and Steve K.W. Oh -- Loss-of-function studies in mouse embryonic stem cells using the pHYPER shRNA plasmic vector / Soizik Berlivet, Martin Houlard, and Matthieu Gérard -- Regulation and/or repression of cholinergic differentiation of murine embryonic stem cells using RNAi directed against transcription factor L3/Lhx8 / Takayuki Manabe and Akio Wanaka -- Silencing of rho-GDI[gamma] by RNAi promotes the differentiation of neural stem cells / Jiao Wang, Wei Lu, and Tieqiao Wen -- RNAi knockdown of redox signaling protein Ape1 in the differentiation of mouse embryonic stem cells / Gang-Ming Zou, Cynthia LeBron, and Yumei Fu -- Proteins involved in cell migration from glioblastoma neurospheres analyzed by overexpression and siRNA-mediated knock-down / Carsten Hagemann ... [et al.] -- An efficient transfection method for mouse embryonic stem cells / Jun-Yang Liou, Bor-Sheng Ko, and Tzu-Ching Chang -- Semi-quantitative analysis of transient single-cell gene expression in embryonic stem cells by femtoinjection / Mikako Saito and Hideaki Matsuoka -- Preparation and analysis of microRNA libraries using the Illumiina massively parallel sequencing technology / Ryan D. Morin ... [et al.] -- Assessing in vivo microRNA function in the germline stem cells of the Drosophila ovary / Kin Chan and Hannele Ruohola-Baker -- Monitoring microRNA expression during embryonic stem-cell differentiation using quantitative real-time PCR (qRT-PCR) / Xiaoping Pan ... [et al.] -- Engineering human mesenchymal stem cells to release adenosine using miRNA technology / Gaoying Ren and Detlev Boison -- Efficient gene knockdowns in mouse embryonic stem cells using microRNA-based shRNAs / Jianlong Wang.
Local note	Laupus-WCP
Local note	ECU author
Bibliography note	Includes bibliographical references and index.
ISBN	9781607617686 (hbk.)
ISBN	1607617684 (hbk.)
ISBN	9781607617693 (e-ISBN)
ISBN	1607617692 (e-ISBN)

RNAi and microRNA-mediated gene regulation in stem cells : methods, protocols, and applications / edited by Baohong Zhang and Edmund J. Stellwag.

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