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035 a| (Sirsi) o664860275;

035 a| (OCoLC)664860275;

040 a| EREc| EREd| EREd| UtOrBLW;

049 a| EREE;

090 a| QH447;

100 1 a| Henderson, Laura M.?| UNAUTHORIZED;

245 10 a| Psf2, a subunit of the heterotetrameric GINS complex, plays a role in cell cycle progression and maintenance of genomic integrity /c| by Laura M Henderson.;

260 a| [Greenville, N.C.] :b| East Carolina University,c| 2010.;

300 a| 64 pages :b| illustrations (some color), digital, PDF file;

336 a| text2| rdacontent;

337 a| computer2| rdamedia;

338 a| online resource2| rdacarrier;

500 a| Presented to the faculty of the Department of Biology.;

500 a| Advisor: Tim Christensen.;

500 a| Title from PDF t.p. (viewed Jan. 4, 2011).;

502 b| M.S.c| East Carolina Universityd| 2010.;

504 a| Includes bibliographical references.;

520 3 a| Multiple proteins are involved in the complete and accurate replication of the genome during S phase of the cell cycle. At the G1/S phase transition, the heterotetrameric GINS complex is recruited to the origin, and facilitates the helicase activity of the Mcm2-7 complex. Each of the four subunits of the GINS complex is essential for proper completion of DNA replication initiation and elongation. Inaccurate replication of the genome results in a multitude of disease states, specifically, cancer. Psf2, a subunit of the GINS complex, has been previously implicated in the segregation of chromosomes during M phase. Additionally, it has been shown that reduced levels of Psf2 in yeast results in stalled replication forks and incomplete DNA replication. However, to date, the function of Psf2 in higher eukaryotes has only been studied in tissue culture models. To provide insight into the role of Psf2 in a multicellular organism, we used an in vivo approach to characterize the phenotypes resulting from the C-terminal truncation of Psf2 in a homozygous lethal mutant in Drosophila. Through analysis of larval brain tissue, salivary tissue, ovarioles, and embryonic tissue, we found the mutant Psf2 displays defects during M phase of the cell cycle and DNA replication in endoreplicating cells. Curiously, the RNAi knockdown of Psf2 results in a defect in S phase of the cell cycle, with no effects on M phase. Therefore, we hypothesize that Psf2 plays an essential role in cell cycle progression. Additionally, removal of the C-terminal domain is essential for either the correct formation of the GINS complex, or for an external interaction, possibly with checkpoint or chromosome segregation proteins.;

538 a| System requirements: Adobe Reader.;

538 a| Mode of access: World Wide Web.;

650 0 a| Genomics.=| ^A528942;

650 0 a| Genomes.=| ^A268444;

650 0 a| Proteins.=| ^A2392;

650 0 a| DNA replicationx| Regulation.=| ^A1036899;

650 0 a| Cell cyclex| Regulation.=| ^A871242;

650 0 a| Eukaryotic cells.=| ^A12878;

653 a| Biology, Molecular;

653 a| Biology, Cell;

700 1 a| Christensen, Tim.=| ^A1389912;

710 2 a| East Carolina University.b| Department of Biology.=| ^A637467;

856 40 z| Access via ScholarShipu| http://hdl.handle.net/10342/2877;

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999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 2329409-2001l| HSLELECm| HSLr| Ys| Yt| HEETDu| 11/3/2010x| ETDz| HERESOURCE;

Psf2, a subunit of the heterotetrameric GINS complex, plays a role in cell cycle progression and maintenance of genomic integrity / by Laura M Henderson.

Author/creator	Henderson, Laura M.
Other author	Christensen, Tim.
Other author	East Carolina University. Department of Biology.
Format	Theses and dissertations
Publication Info	[Greenville, N.C.] : East Carolina University, 2010.
Description	64 pages : illustrations (some color), digital, PDF file
Supplemental Content	Access via ScholarShip
Subjects	Genomics. Genomes. Proteins. DNA replication--Regulation. Cell cycle--Regulation. Eukaryotic cells.

Summary	Multiple proteins are involved in the complete and accurate replication of the genome during S phase of the cell cycle. At the G1/S phase transition, the heterotetrameric GINS complex is recruited to the origin, and facilitates the helicase activity of the Mcm2-7 complex. Each of the four subunits of the GINS complex is essential for proper completion of DNA replication initiation and elongation. Inaccurate replication of the genome results in a multitude of disease states, specifically, cancer. Psf2, a subunit of the GINS complex, has been previously implicated in the segregation of chromosomes during M phase. Additionally, it has been shown that reduced levels of Psf2 in yeast results in stalled replication forks and incomplete DNA replication. However, to date, the function of Psf2 in higher eukaryotes has only been studied in tissue culture models. To provide insight into the role of Psf2 in a multicellular organism, we used an in vivo approach to characterize the phenotypes resulting from the C-terminal truncation of Psf2 in a homozygous lethal mutant in Drosophila. Through analysis of larval brain tissue, salivary tissue, ovarioles, and embryonic tissue, we found the mutant Psf2 displays defects during M phase of the cell cycle and DNA replication in endoreplicating cells. Curiously, the RNAi knockdown of Psf2 results in a defect in S phase of the cell cycle, with no effects on M phase. Therefore, we hypothesize that Psf2 plays an essential role in cell cycle progression. Additionally, removal of the C-terminal domain is essential for either the correct formation of the GINS complex, or for an external interaction, possibly with checkpoint or chromosome segregation proteins.
General note	Presented to the faculty of the Department of Biology.
General note	Advisor: Tim Christensen.
General note	Title from PDF t.p. (viewed Jan. 4, 2011).
Dissertation note	M.S. East Carolina University 2010.
Bibliography note	Includes bibliographical references.
Technical details	System requirements: Adobe Reader.
Technical details	Mode of access: World Wide Web.

Psf2, a subunit of the heterotetrameric GINS complex, plays a role in cell cycle progression and maintenance of genomic integrity / by Laura M Henderson.

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