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003 OCoLC;

005 20141212054934.0;

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008 110125s2010 ncuad ob 000 0 eng d;

035 a| (Sirsi) o698261414;

035 a| (OCoLC)698261414;

040 a| NEHc| NEHd| UtOrBLW;

049 a| NEHH;

066 c| (S;

100 1 a| Wang, Xiaojia.=| ^A1031646;

245 10 a| Circadian activation of mast cells mediates the nocturnal response in allergic asthma /c| by Xiaojia Wang.;

260 a| [Greenville, N.C.] :b| East Carolina University,c| 2010.;

300 a| 145 pages :b| digital, PDF file;

336 a| text2| rdacontent;

337 a| computer2| rdamedia;

338 a| online resource2| rdacarrier;

500 a| Presented to the faculty of the Department of Physiology.;

500 a| Advisor: Michael R. Van Scott.;

500 a| Title from PDF t.p. (viewed January 07, 2011).;

502 b| Ph.D.c| East Carolina Universityd| 2010.;

504 a| Includes bibliographical references.;

538 a| System requirements: Adobe Reader.;

538 a| Mode of access: World Wide Web.;

650 2 a| Circadian Rhythm.=| ^A919175;

650 2 a| Allergens.=| ^A919535;

650 2 a| Asthma.=| ^A918592;

650 2 a| Pyroglyphidae.=| ^A1032972;

650 2 a| Plethysmography.=| ^A1032885;

650 2 a| Airway Obstruction.=| ^A919785;

650 2 a| Bronchoconstriction.=| ^A1032934;

650 2 a| Bronchoconstrictor Agents.=| ^A1032935;

650 2 a| Methacholine Chloride.=| ^A1032936;

650 2 a| Albuterol.=| ^A930948;

650 2 a| Sleepx| physiology.=| ^A919039;

653 a| Health Sciences, Immunology;

700 1 a| Van Scott, Michael R.?| UNAUTHORIZED;

710 2 a| East Carolina University.b| Department of Physiology.?| UNAUTHORIZED;

856 40 z| Access via ScholarShipu| http://hdl.handle.net/10342/2962;

880 3 6| 520-00/(Sa| Circadian rhythm is a conserved physiological feature of most organisms. Nocturnal symptoms are a common feature of allergic asthma in humans. Patients with nocturnal asthma show circadian variation of their pulmonary function and inflammation, but nocturnal asthmatic responses have not been documented in animal models. In this study we tested the hypotheses that house dust mite (HDM) allergen-sensitive nonhuman primates (NHP) manifest nocturnal symptoms, and whether intrinsic circadian activity in mast cells might underlie nocturnal asthma response. Dynamic compliance (C dyn ) and lung resistance (RL ) measured as indicators of bronchoconstriction in anesthetized NHP, at rest and following exposure to HDM allergen, methacholine, and albuterol, were highly correlated with 3 respiratory inductive plethysmography (RIP) parameters: Phase Angle of the rib cage and abdomen waveforms (PhAng), Baseline Effort Phase Relation (eBPRL) and Effort Phase Relation (ePhRL). 21 allergic subjects were challenged with HDM early in the morning, and eBPRL and ePhRL were monitored for 20 hours after provocation. 15 of the allergic subjects exhibited gradual increases in eBPRL and ePhRL between midnight and 6 AM, with peak activity at 4 AM. The results demonstrate that animals exhibiting acute responses to allergen exposure during the day also exhibit nocturnal airway obstruction, possibly resulting from bronchoconstriction. Mast cells are central effector cells in asthma, and were subsequently investigated for intrinsic circadian activity that could underlie nocturnal asthma responses. Bone marrow derived mast cells (BMMCs) were exposed to high concentrations of serum (serum shocked) to synchronize gene expression. Following serum shock, circadian genes (mPer2, Bmal1, Rev-erbα, and Dbp) exhibit oscillations for up to 72 hours. Likewise, the high affinity IgE receptor in BMMCs (FcεRIα) exhibited circadian expression. The fcer1a gene and FcεRIα protein following serum shock exhibited mean periods of 18.9 and 28.6 hours, respectively. Synchronized BMMCs stimulated with IgE/Ag at different circadian time display circadian rhythms in IL-13 mRNA. Taken together, synchronized BMMCs suggest an underlying circadian mechanism of nocturnal asthma in vitro.;

949 a| Click on web addressw| ASISh| JOYNER101;

949 a| Click on web addressw| ASISh| HSL111;

994 a| C0b| NEH;

096 a| WF 553;

596 a| 1 4;

998 a| 2397262;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 2397262-1001l| JNETm| JOYNERr| Ys| Yt| JNE3ETDu| 1/25/2011x| ETDz| JERESOURCE;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 2397262-2001l| HSLELECm| HSLr| Ys| Yt| HEETDu| 1/25/2011x| ETDz| HERESOURCE;

Circadian activation of mast cells mediates the nocturnal response in allergic asthma / by Xiaojia Wang.

Author/creator	Wang, Xiaojia
Other author	Van Scott, Michael R.
Other author	East Carolina University. Department of Physiology.
Format	Theses and dissertations
Publication Info	[Greenville, N.C.] : East Carolina University, 2010.
Description	145 pages : digital, PDF file
Supplemental Content	Access via ScholarShip
Subjects	Circadian Rhythm. Allergens. Asthma. Pyroglyphidae. Plethysmography. Airway Obstruction. Bronchoconstriction. Bronchoconstrictor Agents. Methacholine Chloride. Albuterol. Sleep--physiology.

General note	Presented to the faculty of the Department of Physiology.
General note	Advisor: Michael R. Van Scott.
General note	Title from PDF t.p. (viewed January 07, 2011).
Dissertation note	Ph.D. East Carolina University 2010.
Bibliography note	Includes bibliographical references.
Technical details	System requirements: Adobe Reader.
Technical details	Mode of access: World Wide Web.

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Circadian activation of mast cells mediates the nocturnal response in allergic asthma / by Xiaojia Wang.

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