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001 ocn733829504;

003 OCoLC;

005 20141212065445.0;

006 m d ;

007 cr bn|||||||||;

008 110630s2010 ncua ob 000 0 eng d;

035 a| (Sirsi) o733829504;

035 a| (OCoLC)733829504;

040 a| EREc| EREd| EREd| UtOrBLW;

049 a| EREE;

090 a| QP606.D46;

100 1 a| Gosnell, Justin A.?| UNAUTHORIZED;

245 10 a| Drosophila Ctf4 is essential for genome stability and normal cell cycle progression /c| by Justin A. Gosnell.;

246 1 i| title from abstract pagea| Drosophila Ctf4 is essential for efficient DNA replication and normal cell cycle progression;

260 a| [Greenville, N.C.] :b| East Carolina University,c| 2010.;

300 a| 56 pages :b| illustrations (some color), digital, PDF file;

336 a| text2| rdacontent;

337 a| computer2| rdamedia;

338 a| online resource2| rdacarrier;

538 a| System requirements: Adobe Reader.;

538 a| Mode of access: World Wide Web.;

502 b| M.S.c| East Carolina Universityd| 2010.;

500 a| Presented to the faculty of the Department of Biology.;

500 a| Advisor: Tim Christensen.;

500 a| Title from PDF t.p. (viewed July 21, 2011).;

520 3 a| Proper DNA replication and well-timed cell cycle progression are vital to the normal functioning of a cell. Precise coordination between these mechanisms' constituent proteins ensures their processivity while safeguarding against DNA damage. The Ctf4 protein is a central member of the replication fork and links the replicative MCM helicase and polymerase [alpha]-primase. In addition, it has been implicated as a member of a complex that promotes replication fork stability, the Fork Protection Complex (FPC). This investigation represents the first phenotypic analysis of the function of the Ctf4 protein within a multicellular organism model. We show that Ctf4 interacts with Polymerase [alpha], MCM2, Psf1, and Psf2. We also demonstrate that knockdown of this central replication fork component via a GAL4-UAS RNAi system results in a lower frequency of mitosis due to an S-phase delay, endoreplication defects, as well as mitotic bridging in early embryonic development.;

504 a| Includes bibliographical references.;

650 0 a| DNA polymerases.=| ^A284054;

650 0 a| Protein binding.=| ^A12889;

650 0 a| DNA replication.=| ^A825742;

650 0 a| Cell cycle.=| ^A4972;

650 0 a| Eukaryotic cells.=| ^A12878;

650 0 a| Genomes.=| ^A268444;

650 0 a| Drosophila.=| ^A54199;

653 a| Molecular Biology;

653 a| Genetics;

653 a| Biology;

700 1 a| Christensen, Tim.=| ^A1389912;

710 2 a| East Carolina University.b| Department of Biology.=| ^A637467;

856 40 z| Access via ScholarShipu| http://hdl.handle.net/10342/3560;

949 o| jgml;

994 a| C0b| ERE;

596 a| 1 4;

998 a| 2507463;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 2507463-1001l| JNETm| JOYNERr| Ys| Yt| JNE3ETDu| 6/30/2011x| ETDz| JERESOURCE;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 2507463-2001l| HSLELECm| HSLr| Ys| Yt| HEETDu| 6/30/2011x| ETDz| HERESOURCE;

Drosophila Ctf4 is essential for genome stability and normal cell cycle progression / by Justin A. Gosnell.

Author/creator	Gosnell, Justin A.
Other author	Christensen, Tim.
Other author	East Carolina University. Department of Biology.
Format	Theses and dissertations
Publication Info	[Greenville, N.C.] : East Carolina University, 2010.
Description	56 pages : illustrations (some color), digital, PDF file
Supplemental Content	Access via ScholarShip
Subjects	DNA polymerases. Protein binding. DNA replication. Cell cycle. Eukaryotic cells. Genomes. Drosophila.

Variant title	title from abstract page Drosophila Ctf4 is essential for efficient DNA replication and normal cell cycle progression
Summary	Proper DNA replication and well-timed cell cycle progression are vital to the normal functioning of a cell. Precise coordination between these mechanisms' constituent proteins ensures their processivity while safeguarding against DNA damage. The Ctf4 protein is a central member of the replication fork and links the replicative MCM helicase and polymerase [alpha]-primase. In addition, it has been implicated as a member of a complex that promotes replication fork stability, the Fork Protection Complex (FPC). This investigation represents the first phenotypic analysis of the function of the Ctf4 protein within a multicellular organism model. We show that Ctf4 interacts with Polymerase [alpha], MCM2, Psf1, and Psf2. We also demonstrate that knockdown of this central replication fork component via a GAL4-UAS RNAi system results in a lower frequency of mitosis due to an S-phase delay, endoreplication defects, as well as mitotic bridging in early embryonic development.
General note	Presented to the faculty of the Department of Biology.
General note	Advisor: Tim Christensen.
General note	Title from PDF t.p. (viewed July 21, 2011).
Dissertation note	M.S. East Carolina University 2010.
Bibliography note	Includes bibliographical references.
Technical details	System requirements: Adobe Reader.
Technical details	Mode of access: World Wide Web.

Drosophila Ctf4 is essential for genome stability and normal cell cycle progression / by Justin A. Gosnell.

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