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LEADER 03670cam 2200493Ia 4500

001 ocn733829510;

003 OCoLC;

005 20141212065446.0;

006 m d ;

007 cr bn|||||||||;

008 110630s2011 ncua ob 000 0 eng d;

035 a| (Sirsi) o733829510;

035 a| (OCoLC)733829510;

040 a| EREc| EREd| EREd| UtOrBLW;

049 a| EREE;

090 a| QR201.B8;

100 1 a| Menscher, Evan A.?| UNAUTHORIZED;

245 14 a| The fur-like regulator mur controls the expression of the gene encoding the manganese transporter MntH in Brucella abortus 2308 in response to manganese availability /c| by Evan A. Menscher.;

260 a| [Greenville, N.C.] :b| East Carolina University,c| 2011.;

300 a| 177 pages :b| illustrations (some color), digital, PDF file;

336 a| text2| rdacontent;

337 a| computer2| rdamedia;

338 a| online resource2| rdacarrier;

538 a| System requirements: Adobe Reader.;

538 a| Mode of access: World Wide Web.;

502 b| M.S.c| East Carolina Universityd| 2011.;

500 a| Presented to the faculty of the Department of Biology.;

500 a| Advisor: Roy Roop II.;

500 a| Title from PDF t.p. (viewed July 28, 2011).;

520 3 a| Brucella abortus is a Gram-negative, [alpha]-proteobacterium that causes bovine brucellosis, a prevalent zoonotic disease in many areas of the world. Brucella abortus is an intracellular pathogen that survives and replicates in the phagosomal compartment of host macrophages, a property that is critical for virulence. In this environment, Brucella abortus requires manganese, but the availability of this essential micronutrient is restricted in the phagosomal compartment. Host cells resist bacterial infection by limiting the availability of metals in the phagosome employing what is known as the metal withholding defense, a process mediated by Nramp1 (Natural resistance associated macrophage protein). To counter this, pathogenic bacteria produce high-affinity manganese transporters, and these transporters have been shown to be necessary for virulence. MntH is the bacterial homolog of the eukaryotic Nramp and is the only identified high affinity manganese transporter in Brucella strains. In this study, the function and the metal-specificity of this protein was examined. Studies employing a genetically defined Brucella abortus mntH mutant show that MntH-mediated manganese acquisition is critical for normal in vitro growth and the establishment of infection in mice. The [alpha]-proteobacteria employ a structural homolog of the ferric uptake regulator Fur which is Mn-responsive (and thus designated Mur for manganese uptake regulator) to control the expression of their manganese acquisition genes. Phenotypic analysis of a mur mutant showed that Mur is responsible for the Mn-responsive regulation of mntH expression in B. abortus 2308 and biochemical studies defined the Mur binding site in the mntH promoter.;

504 a| Includes bibliographical references.;

650 0 a| Brucella abortus.=| ^A784450;

650 0 a| Manganese.=| ^A89270;

650 0 a| Macrophages.=| ^A3272;

653 a| Microbiology;

700 1 a| Roop, R. Martin,c| II.=| ^A1383281;

710 2 a| East Carolina University.b| Department of Biology.=| ^A637467;

856 40 z| Access via ScholarShipu| http://hdl.handle.net/10342/3561;

949 o| jgml;

994 a| C0b| ERE;

596 a| 1 4;

998 a| 2507475;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 2507475-1001l| JNETm| JOYNERr| Ys| Yt| JNE3ETDu| 6/30/2011x| ETDz| JERESOURCE;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 2507475-2001l| HSLELECm| HSLr| Ys| Yt| HEETDu| 6/30/2011x| ETDz| HERESOURCE;

The fur-like regulator mur controls the expression of the gene encoding the manganese transporter MntH in Brucella abortus 2308 in response to manganese availability / by Evan A. Menscher.

Author/creator	Menscher, Evan A.
Other author	Roop, R. Martin, II.
Other author	East Carolina University. Department of Biology.
Format	Theses and dissertations
Publication Info	[Greenville, N.C.] : East Carolina University, 2011.
Description	177 pages : illustrations (some color), digital, PDF file
Supplemental Content	Access via ScholarShip
Subjects	Brucella abortus. Manganese. Macrophages.

Summary	Brucella abortus is a Gram-negative, [alpha]-proteobacterium that causes bovine brucellosis, a prevalent zoonotic disease in many areas of the world. Brucella abortus is an intracellular pathogen that survives and replicates in the phagosomal compartment of host macrophages, a property that is critical for virulence. In this environment, Brucella abortus requires manganese, but the availability of this essential micronutrient is restricted in the phagosomal compartment. Host cells resist bacterial infection by limiting the availability of metals in the phagosome employing what is known as the metal withholding defense, a process mediated by Nramp1 (Natural resistance associated macrophage protein). To counter this, pathogenic bacteria produce high-affinity manganese transporters, and these transporters have been shown to be necessary for virulence. MntH is the bacterial homolog of the eukaryotic Nramp and is the only identified high affinity manganese transporter in Brucella strains. In this study, the function and the metal-specificity of this protein was examined. Studies employing a genetically defined Brucella abortus mntH mutant show that MntH-mediated manganese acquisition is critical for normal in vitro growth and the establishment of infection in mice. The [alpha]-proteobacteria employ a structural homolog of the ferric uptake regulator Fur which is Mn-responsive (and thus designated Mur for manganese uptake regulator) to control the expression of their manganese acquisition genes. Phenotypic analysis of a mur mutant showed that Mur is responsible for the Mn-responsive regulation of mntH expression in B. abortus 2308 and biochemical studies defined the Mur binding site in the mntH promoter.
General note	Presented to the faculty of the Department of Biology.
General note	Advisor: Roy Roop II.
General note	Title from PDF t.p. (viewed July 28, 2011).
Dissertation note	M.S. East Carolina University 2011.
Bibliography note	Includes bibliographical references.
Technical details	System requirements: Adobe Reader.
Technical details	Mode of access: World Wide Web.

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The fur-like regulator mur controls the expression of the gene encoding the manganese transporter MntH in Brucella abortus 2308 in response to manganese availability / by Evan A. Menscher.

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