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001 ocn747860684;

003 OCoLC;

005 20141212070851.0;

006 m d ;

007 cr bn|||||||||;

008 110825s2011 ncua ob 000 0 eng d;

035 a| (Sirsi) o747860684;

035 a| (OCoLC)747860684;

040 a| EREc| EREd| EREd| UtOrBLW;

049 a| EREE;

090 a| QP606.D46;

100 1 a| Hunter, Chad Michael.?| UNAUTHORIZED;

245 10 a| Characterization of two novel mutants of DNA polymerase delta in Drosophila melanogaster /c| by Chad Michael Hunter.;

260 a| [Greenville, N.C.] :b| East Carolina University,c| 2011.;

300 a| 137 pages :b| illustrations (some color), digital, PDF file;

336 a| text2| rdacontent;

337 a| computer2| rdamedia;

338 a| online resource2| rdacarrier;

538 a| System requirements: Adobe Reader.;

538 a| Mode of access: World Wide Web.;

502 b| M.S.c| East Carolina Universityd| 2011.;

500 a| Presented to the faculty of the Department of Biology.;

500 a| Advisor: Tim Christensen.;

500 a| Title from PDF t.p. (viewed Oct. 5, 2011).;

520 3 a| DNA is the genetic material for all living organisms which is constantly being unpackaged, replicated and repackaged. The replication of this genetic material involves numerous different proteins; however, DNA polymerase delta (pol [delta]) carries much of the load by replicating a major portion of the genome in both leading and lagging strand synthesis. Using the model organism, Drosophila melanogaster, we investigate two novel mutations in two different evolutionary conserved regions. One region corresponds with the polymerase's ability to polymerize new nucleotides onto an existing strand of DNA. The other region corresponds with the polymerase's ability to proofread in the 3' to 5' direction. These two mutants, both homozygous lethal and recessive, show interesting phenotypes with a delay in S-phase, numerous chromosome aberrations, defects in endoreplication and possible protection form DNA damage. Using these two mutants, these two domains can be further characterized. By understanding how pol [delta] functions in an in vivo setting, we can apply this knowledge to the mechanics of cancer biology in humans, another multicelluar organism, and inform new therapies to treat it.;

504 a| Includes bibliographical references.;

650 0 a| DNA polymerases.=| ^A284054;

650 0 a| Mutation (Biology)=| ^A2390;

650 0 a| Drosophila melanogaster.=| ^A86209;

653 a| Genetics;

653 a| Biology;

653 a| Molecular Biology;

700 1 a| Christensen, Tim.=| ^A1389912;

710 2 a| East Carolina University.b| Department of Biology.=| ^A637467;

856 40 z| Access via ScholarShipu| http://hdl.handle.net/10342/3627;

949 o| jgml;

994 a| C0b| ERE;

596 a| 1 4;

998 a| 2533141;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 2533141-1001l| JNETm| JOYNERr| Ys| Yt| JNE3ETDu| 8/26/2011x| ETDz| JERESOURCE;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 2533141-2001l| HSLELECm| HSLr| Ys| Yt| HEETDu| 8/26/2011x| ETDz| HERESOURCE;

Characterization of two novel mutants of DNA polymerase delta in Drosophila melanogaster / by Chad Michael Hunter.

Author/creator	Hunter, Chad Michael
Other author	Christensen, Tim.
Other author	East Carolina University. Department of Biology.
Format	Theses and dissertations
Publication Info	[Greenville, N.C.] : East Carolina University, 2011.
Description	137 pages : illustrations (some color), digital, PDF file
Supplemental Content	Access via ScholarShip
Subjects	DNA polymerases. Mutation (Biology) Drosophila melanogaster.

Summary	DNA is the genetic material for all living organisms which is constantly being unpackaged, replicated and repackaged. The replication of this genetic material involves numerous different proteins; however, DNA polymerase delta (pol [delta]) carries much of the load by replicating a major portion of the genome in both leading and lagging strand synthesis. Using the model organism, Drosophila melanogaster, we investigate two novel mutations in two different evolutionary conserved regions. One region corresponds with the polymerase's ability to polymerize new nucleotides onto an existing strand of DNA. The other region corresponds with the polymerase's ability to proofread in the 3' to 5' direction. These two mutants, both homozygous lethal and recessive, show interesting phenotypes with a delay in S-phase, numerous chromosome aberrations, defects in endoreplication and possible protection form DNA damage. Using these two mutants, these two domains can be further characterized. By understanding how pol [delta] functions in an in vivo setting, we can apply this knowledge to the mechanics of cancer biology in humans, another multicelluar organism, and inform new therapies to treat it.
General note	Presented to the faculty of the Department of Biology.
General note	Advisor: Tim Christensen.
General note	Title from PDF t.p. (viewed Oct. 5, 2011).
Dissertation note	M.S. East Carolina University 2011.
Bibliography note	Includes bibliographical references.
Technical details	System requirements: Adobe Reader.
Technical details	Mode of access: World Wide Web.

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Characterization of two novel mutants of DNA polymerase delta in Drosophila melanogaster / by Chad Michael Hunter.

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