Cyclic adenosine 3',5'-monophosphate : effect on neurulation in the early chick embryo / by David R. Bjorkman.
| Author/creator | Bjorkman, David R. author. |
| Other author | Kalmus, Gerhard W., degree supervisor. |
| Other author | East Carolina University. Department of Biology. |
| Format | Theses and dissertations |
| Production | 1983. |
| Description | vii, 97 leaves : illustrations ; 28 cm |
| Supplemental Content | Access via ScholarShip |
| Subjects |
| Summary | Cyclic adenosine 3',5'-monophosphate (cyclic AMP, cAMP) concentration and distribution were determined in the chick embryo during the formation of the central nervous system. An axial gradient of cAMP concentration was found along the developing neural tube by radloimmunoassay. A second axial gradient was found by immunohistochemical localization of bound cAMP. During the process of neural tube closure cAMP concentration was found to decrease while Intracellular cAMP distribution was found to change from primarily cytoplasmic to membrane bound. When the cAMP gradients were disturbed by treatment of embryos In culture with concanavalln A (Con A) or exogenous cAMP, the neural tube failed to close in 60-90^ of the embryos. Con A and exogenous cAMP treatments were found to alter the Intracellular distribution and the axial gradient of bound cAMP. This interference with the normal patterns of cAMP distribution may inhibit neural tube closure through a mechanism involving cAMP-dependent-microtubule assembly and -microfilament constriction. |
| General note | "Presented to the faculty of the Department of Biology ... in partial fulfillment of the requirements for the degree Master of Science in Biology." |
| General note | Advisor: Gerhard W. Kalmus |
| Dissertation note | M.S. East Carolina University 1983 |
| Bibliography note | Includes bibliographical references (leaves 86-97). |
| Genre/form | Academic theses. |
| Genre/form | Academic theses. |
| Genre/form | Thèses et écrits académiques. |