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LEADER 04875ctm 2200541 i 4500

001 ocm10809536;

003 OCoLC;

005 20251021154321.0;

008 840606s1983 xx a bm 000 0 eng d;

035 a| (Sirsi) o10809536;

035 a| (OCoLC)10809536;

049 a| EREE;

050 4 a| QR185.8.L93b| L37x 1983;

100 1 a| Latta, Harold Frank,c| III,e| author.?| UNAUTHORIZED;

245 10 a| Partial characterization of sheep T cell growth factor /c| by Harold Frank Latta, III.;

264 0 c| 1983.;

300 a| viii, 106 leaves :b| illustrations ;c| 28 cm;

336 a| textb| txt2| rdacontent;

337 a| unmediatedb| n2| rdamedia;

338 a| volumeb| nc2| rdacarrier;

502 b| M.S.c| East Carolina Universityd| 1983;

500 a| "Presented to the faculty of the Department of Biology ... in partial fulfillment of the requirements for the degree Master of Science in Biology.";

500 a| Advisors: Leonard S. English, Gerhard W. Kalmus;

520 3 a| T cell growth factor (TCGF) is a lymphokine which plays an important role in the regulation of the immune response and has been partially characterized in man, mouse and rat. The unique property of TCGF is its ability to support the growth of activated T cells. In this work, the following characteristics of sheep TCGF were examined: the biochemical properties, the interspecies activity, and the susceptibility of sheep TCGF activity to Inhibition by monosaccharides. To facilitate the characterization studies, the optimal conditions for sheep TCGF were established and were used as a standard method to pre- pare crude TCGF supernatants (Sn's). This standard method consisted of a 48 hour culture of peripheral blood lymphocytes in RPMI 1640 at 5 x 106 cells/ml in the presence of 2% fetal calf serum, 4 ug/ml Concanava- lin A and 0.1 mg/ml polyethylene glycol 6000. The Interspecies activity of sheep TCGF was determined by culturing human, sheep, rat and mouse activated T cells in the presence of sheep Sn. TCGF supernatants from the other species were also tested to provide a comparative study. It was found that sheep TCGF could only support the growth of activated sheep cells, but sheep activated cells could proliferate in response to human, rat and sheep TCGF's. In contrast, it was found that human TCGF could support the growth of activated cells from all 4 species, but human cells could only respond to human TCGF. The biochemical studies of sheep TCGF revealed that sheep TCGF was precipitated by 60-80% saturation of ammonium sulfate, was eluted between 0-0.15 NaCl in DEAE-cellulose chromatography, possessed an apparent molecular weight of 30,000d and possessed isoelectric points in the range of 7.1-7.9 and 5.1-5.6. Initial studies were conducted to determine if the charge heterogeneity of sheep TCGF was due to residual ConA or was due to different molecular species of sheep TCGF. It was demonstrated that the addition of 0.1M alpha methyl mannoside resulted in a significant inhibition of sheep TCGF activity. Experiments were conducted which confirmed that alpha methyl mannoside inhibition was not due to residual ConA. This study provides strong evidence that alpha methyl mannoside interferes with the binding of sheep TCGF to the TCGF receptor on the surface of the activated T cells.;

504 a| Includes bibliographical references (leaves 96-106).;

650 0 a| Lymphokines.=| ^A13425;

650 0 a| T cells.=| ^A5851;

650 2 a| Lymphokines.0| (DNLM)D008222=| ^A950014;

655 2 a| Academic Dissertation.0| (DNLM)D019478?| UNAUTHORIZED;

650 7 a| Lymphokines.2| fast0| (OCoLC)fst01004317;

650 7 a| T cells.2| fast0| (OCoLC)fst01141544;

655 7 a| Academic theses.2| fast0| (OCoLC)fst01726453;

655 7 a| Academic theses.2| lcgft;

655 7 a| Thèses et écrits académiques.2| rvmgf0| (CaQQLa)RVMGF-000001173;

700 1 a| English, Leonard S.,e| degree supervisor.?| UNAUTHORIZED;

700 1 a| Kalmus, Gerhard W.,e| degree supervisor.?| UNAUTHORIZED;

710 2 a| East Carolina University.b| Department of Biology.=| ^A637467;

856 41 z| Access via ScholarShipu| http://hdl.handle.net/10342/11223;

949 a| Click on web addressw| asish| joyner101;

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Partial characterization of sheep T cell growth factor / by Harold Frank Latta, III.

Author/creator	Latta, Harold Frank author.
Other author	English, Leonard S., degree supervisor.
Other author	Kalmus, Gerhard W., degree supervisor.
Other author	East Carolina University. Department of Biology.
Format	Theses and dissertations
Production	1983.
Description	viii, 106 leaves : illustrations ; 28 cm
Supplemental Content	Access via ScholarShip
Subjects	Lymphokines. T cells. Lymphokines.

Summary	T cell growth factor (TCGF) is a lymphokine which plays an important role in the regulation of the immune response and has been partially characterized in man, mouse and rat. The unique property of TCGF is its ability to support the growth of activated T cells. In this work, the following characteristics of sheep TCGF were examined: the biochemical properties, the interspecies activity, and the susceptibility of sheep TCGF activity to Inhibition by monosaccharides. To facilitate the characterization studies, the optimal conditions for sheep TCGF were established and were used as a standard method to pre- pare crude TCGF supernatants (Sn's). This standard method consisted of a 48 hour culture of peripheral blood lymphocytes in RPMI 1640 at 5 x 106 cells/ml in the presence of 2% fetal calf serum, 4 ug/ml Concanava- lin A and 0.1 mg/ml polyethylene glycol 6000. The Interspecies activity of sheep TCGF was determined by culturing human, sheep, rat and mouse activated T cells in the presence of sheep Sn. TCGF supernatants from the other species were also tested to provide a comparative study. It was found that sheep TCGF could only support the growth of activated sheep cells, but sheep activated cells could proliferate in response to human, rat and sheep TCGF's. In contrast, it was found that human TCGF could support the growth of activated cells from all 4 species, but human cells could only respond to human TCGF. The biochemical studies of sheep TCGF revealed that sheep TCGF was precipitated by 60-80% saturation of ammonium sulfate, was eluted between 0-0.15 NaCl in DEAE-cellulose chromatography, possessed an apparent molecular weight of 30,000d and possessed isoelectric points in the range of 7.1-7.9 and 5.1-5.6. Initial studies were conducted to determine if the charge heterogeneity of sheep TCGF was due to residual ConA or was due to different molecular species of sheep TCGF. It was demonstrated that the addition of 0.1M alpha methyl mannoside resulted in a significant inhibition of sheep TCGF activity. Experiments were conducted which confirmed that alpha methyl mannoside inhibition was not due to residual ConA. This study provides strong evidence that alpha methyl mannoside interferes with the binding of sheep TCGF to the TCGF receptor on the surface of the activated T cells.
General note	"Presented to the faculty of the Department of Biology ... in partial fulfillment of the requirements for the degree Master of Science in Biology."
General note	Advisors: Leonard S. English, Gerhard W. Kalmus
Dissertation note	M.S. East Carolina University 1983
Bibliography note	Includes bibliographical references (leaves 96-106).
Genre/form	Academic theses.
Genre/form	Academic theses.
Genre/form	Thèses et écrits académiques.

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Partial characterization of sheep T cell growth factor / by Harold Frank Latta, III.

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