Bacillus Subtilis iron operon mutants produced by treatment with 1-methyl-3-nitro-1-nitrosoguanidine / by Mark Steve Nelson.
| Author/creator | Nelson, Mark Steven author. |
| Other author | Ito, Takeru, degree supervisor. |
| Other author | East Carolina University. Department of Biology. |
| Format | Theses and dissertations |
| Production | 1984. |
| Description | 36 leaves : illustrations ; 28 cm |
| Supplemental Content | Access via ScholarShip |
| Subjects |
| Summary | Bacillus subtil̕s, a microorganism which is known to excrete itoic acid, a siderophore, and coproporphyrin when grown in culture media deficient in iron, was treated with l-methyl-3-nitro-l-nitrosoguanidine (NTG) in order to obtain a constitutive mutant that would excrete itoic acid regardless of iron availability. Instead of such a mutant, the treatment produced many itoic acid defective mutants that lost ability to excrete itoic acid, as well as coproporphyrin, under iron deficient conditions. This finding supports the idea that the excretion of itoic acid is genetically controlled by an iron operon whose activities are regulated by iron concentration in the bacterial cell. It also suggests that the excretion of itoic acid and coproporphyrin is regulated at a common genetic locus in the iron operon. One itoic acid defective mutant examined seemingly gained the ability to excrete a yellow pigment that appeared to be similar to a pteridine-containing siderochrome found in Pseudomonas frisellum. Some of the other itoic acid mutants were morphological mutants that could have been defective in the genetic mechanism of cell wall formation. The appearance of the mutants having a variety of phenotypic characteristics in this study supports the fact that there is a wide range of mutations possible with the use of NTG. |
| General note | "Presented to the faculty of the Department of Biology ... in partial fulfillment of the requirements for the degree Master of Science in Biology." |
| General note | Advisor: Takeru Ito |
| Dissertation note | M.S. East Carolina University 1984 |
| Bibliography note | Includes bibliographical references (leaves 34-36). |
| Genre/form | dissertations. |
| Genre/form | Academic theses. |
| Genre/form | Academic theses. |
| Genre/form | Thèses et écrits académiques. |
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