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LEADER 04350ctm 2200565 i 4500

001 ocm12923082;

003 OCoLC;

005 20230809090307.0;

008 851217s1985 xx a bm 000 0 eng d;

035 a| (Sirsi) o12923082;

035 a| (OCoLC)12923082;

049 a| EREE;

050 4 a| QR201.O84b| B42x 1985;

100 1 a| Beamer, Mark Edward,e| author.?| UNAUTHORIZED;

245 10 a| Experimental otitis media in gerbils using single and polymicrobial anaerobic bacterial cultures /c| by Mark Edward Beamer.;

264 0 c| 1985.;

300 a| vii, 36 leaves :b| illustrations (some color) ;c| 28 cm;

336 a| textb| txt2| rdacontent;

337 a| unmediatedb| n2| rdamedia;

338 a| volumeb| nc2| rdacarrier;

502 b| M.S.c| East Carolina Universityd| 1985;

500 a| "Presented to the faculty and Department of Biology in partial fulfillment of the requirements for the degree Master of Science in Biology.";

500 a| Advisor: Robert S. Fulghum;

500 a| Advisor: Wendell E. Allen;

520 3 a| The objective was to determine pathogenic potentials of anaerobic bacteria and known mixtures of anaerobic bacteria to cause otitis media. Gerbils were inoculated per cutaneously into the middle ear bulla with 4.5 X 10 bacteria per ear. Evaluation was by ototoscopic and histologic observations. Nine different anaerobes and one facultatively anaerobic bacterium used were: Peptococcus magnus, Peptostreptococcus anaerobius, Ps. intermedius, Propionibacterium acňs, Gaffyka anaerobios, Clostridium perfringens, Bacteroides fragilis, B. melaninogenicus, Fusobacterium nucleatum and Escherichia coli. The ability of both living and dead cultures, as well as mixed cultures, to cause otitis media was compared using a predetermined scoring system. The time course and sequellae (persistant granulation tissue, bone damage, and new bone formation) of the otitis media were similarly compared for a period of up to 4 weeks. Inflammatory responses with no sequellae were produced by 2 of the pure cultures but none of the mixtures. Moderate otitis media was caused by 6 of the pure cultures and 7 of the mixtures. Severe otitis media with granulation tissue and remarkable bone changes were found caused by 2 of the pure cultures and 5 of the mixtures. Synergy appeared to be responsible for more severe otitis media with 5 of the mixtures as compared to the response to the individual pure cultures of the mixture. We conclude that experimental otitis media can be caused by anaerobic bacteria in the gerbil. In humans, natural otitis media caused by or contributed to by anaerobes probably occurs as secondary infections following acute otitis media during which conditions for anaerobes to grow are extablished.;

504 a| Includes bibliographical references (leaves 35-36).;

650 0 a| Otitis mediax| Etiology.=| ^A11249;

650 0 a| Middle earx| Microbiology.=| ^A4358;

650 0 a| Anaerobic bacteria.=| ^A5581;

650 0 a| Pathogenic bacteria.=| ^A3355;

650 7 a| Anaerobic bacteria.2| fast0| (OCoLC)fst00808243;

650 7 a| Pathogenic bacteria.2| fast0| (OCoLC)fst01054930;

655 7 a| Academic theses.2| fast0| (OCoLC)fst01726453;

655 7 a| Academic theses.2| lcgft;

655 7 a| Thèses et écrits académiques.2| rvmgf0| (CaQQLa)RVMGF-000001173;

700 1 a| Fulghum, Robert S.,e| degree supervisor.?| UNAUTHORIZED;

700 1 a| Allen, Wendall E.,e| degree supervisor.?| UNAUTHORIZED;

710 2 a| East Carolina University.b| Department of Biology.=| ^A637467;

856 41 z| Access via ScholarShipu| http://hdl.handle.net/10342/11350;

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949 a| Click on web addressw| asish| hsl111;

994 a| C0b| ERE;

998 a| 264312;

596 a| 1 4;

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Experimental otitis media in gerbils using single and polymicrobial anaerobic bacterial cultures / by Mark Edward Beamer.

Author/creator	Beamer, Mark Edward author.
Other author	Fulghum, Robert S., degree supervisor.
Other author	Allen, Wendall E., degree supervisor.
Other author	East Carolina University. Department of Biology.
Format	Theses and dissertations
Production	1985.
Description	vii, 36 leaves : illustrations (some color) ; 28 cm
Supplemental Content	Access via ScholarShip
Subjects	Otitis media--Etiology. Middle ear--Microbiology. Anaerobic bacteria. Pathogenic bacteria.

Summary	The objective was to determine pathogenic potentials of anaerobic bacteria and known mixtures of anaerobic bacteria to cause otitis media. Gerbils were inoculated per cutaneously into the middle ear bulla with 4.5 X 10 bacteria per ear. Evaluation was by ototoscopic and histologic observations. Nine different anaerobes and one facultatively anaerobic bacterium used were: Peptococcus magnus, Peptostreptococcus anaerobius, Ps. intermedius, Propionibacterium acňs, Gaffyka anaerobios, Clostridium perfringens, Bacteroides fragilis, B. melaninogenicus, Fusobacterium nucleatum and Escherichia coli. The ability of both living and dead cultures, as well as mixed cultures, to cause otitis media was compared using a predetermined scoring system. The time course and sequellae (persistant granulation tissue, bone damage, and new bone formation) of the otitis media were similarly compared for a period of up to 4 weeks. Inflammatory responses with no sequellae were produced by 2 of the pure cultures but none of the mixtures. Moderate otitis media was caused by 6 of the pure cultures and 7 of the mixtures. Severe otitis media with granulation tissue and remarkable bone changes were found caused by 2 of the pure cultures and 5 of the mixtures. Synergy appeared to be responsible for more severe otitis media with 5 of the mixtures as compared to the response to the individual pure cultures of the mixture. We conclude that experimental otitis media can be caused by anaerobic bacteria in the gerbil. In humans, natural otitis media caused by or contributed to by anaerobes probably occurs as secondary infections following acute otitis media during which conditions for anaerobes to grow are extablished.
General note	"Presented to the faculty and Department of Biology in partial fulfillment of the requirements for the degree Master of Science in Biology."
General note	Advisor: Robert S. Fulghum
General note	Advisor: Wendell E. Allen
Dissertation note	M.S. East Carolina University 1985
Bibliography note	Includes bibliographical references (leaves 35-36).
Genre/form	Academic theses.
Genre/form	Academic theses.
Genre/form	Thèses et écrits académiques.

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Experimental otitis media in gerbils using single and polymicrobial anaerobic bacterial cultures / by Mark Edward Beamer.

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