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035 a| (Sirsi) o776688722;

035 a| (OCoLC)776688722;

040 a| NEHc| NEHd| UtOrBLW;

049 a| NEHH;

100 1 a| Dyson, Ossie F.?| UNAUTHORIZED;

245 10 a| Regulation of KSHV replication by glycoprotein B: chronicles of a virus and its host /c| by Ossie F. Dyson.;

260 a| [Greenville, N.C.] :b| East Carolina University,c| 2011.;

300 a| 148 pages :b| digital, PDF file;

336 a| text2| rdacontent;

337 a| computer2| rdamedia;

338 a| online resource2| rdacarrier;

538 a| System requirements: Adobe Reader.;

538 a| Mode of access: World Wide Web.;

502 b| Ph.D.c| East Carolina Universityd| 2011.;

500 a| Presented to the faculty of the Department of Microbiology and Immunology.;

500 a| Advisor: Shaw M. Akula.;

500 a| Title from PDF t.p. (viewed January 26, 2012).;

520 3 a| Kaposi's sarcoma-associated herpesvirus (KSHV), the causative agent of three cancers: Kaposi's sarcoma (KS), primary effusion lymphoma (PEL), and the plasmablastic form of multicentric Castleman disease (MCD), shares many characteristics with other herpesviruses including the unique ability to maneuver between latent and lytic modes of infection. During a latent stage of infection, viruses remain dormant with very few genes being expressed. Alternatively, lytic infection is distinguished by the production of infectious virions and virus dissemination. In KSHV pathogenesis, the majority of infected cells support latent infections while less than 3% of them support spontaneous lytic replication. Regulation of latent and lytic infections involves interactions between virus-encoded and cellular factors. However, the exact mechanism(s) supporting it is poorly understood. In the following study, we provide evidence supporting the role for KSHV-encoded glycoprotein B (gB), a structural protein, to inhibit KSHV reactivation from latency via altering the expression of the cellular transcription factor, Early growth response-1 (Egr-1). We observed Egr-1 to specifically bind to two separate locations on the KSHV-encoded ORF50 promoter which plays a significant role in KSHV reactivation. Furthermore, we provide evidence supporting the potential mechanism for KSHV gB to regulate KSHV reactivation. These studies provide new insights and appreciation for the role of virus-encoded structural proteins to support virus latency and may contribute to the development of future therapies.;

504 a| Includes bibliographical references.;

650 2 a| Herpesvirus 8, Human.=| ^A947360;

650 2 a| Sarcoma, Kaposi.=| ^A964398;

650 2 a| Viral Proteins.=| ^A934559;

650 2 a| Basic-Leucine Zipper Transcription Factors.=| ^A1098917;

650 2 a| Castleman Disease=| ^A1202772;

650 2 a| Early Growth Response Protein 1.=| ^A1098918;

650 2 a| Virology.=| ^A918496;

650 2 a| Lymphoma, Primary Effusion.=| ^A1098926;

653 a| Microbiology;

653 a| Virology;

653 a| Molecular biology;

700 1 a| Akula, Shaw M.=| ^A1406820;

710 2 a| East Carolina University.b| Department of Microbiology and Immunology.?| UNAUTHORIZED;

856 40 z| Access via ScholarShipu| http://hdl.handle.net/10342/3728;

949 a| Click on web addressw| ASISh| JOYNER101;

949 a| Click on web addressw| ASISh| HSL111;

994 a| C0b| NEH;

096 a| QZ 345;

596 a| 1 4;

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999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 2685850-1001l| JNETm| JOYNERr| Ys| Yt| JNE3ETDu| 2/15/2012x| ETDz| JERESOURCE;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 2685850-2001l| HSLELECm| HSLr| Ys| Yt| HEETDu| 2/15/2012x| ETDz| HERESOURCE;

Regulation of KSHV replication by glycoprotein B: chronicles of a virus and its host / by Ossie F. Dyson.

Author/creator	Dyson, Ossie F.
Other author	Akula, Shaw M.
Other author	East Carolina University. Department of Microbiology and Immunology.
Format	Theses and dissertations
Publication Info	[Greenville, N.C.] : East Carolina University, 2011.
Description	148 pages : digital, PDF file
Supplemental Content	Access via ScholarShip
Subjects	Herpesvirus 8, Human. Sarcoma, Kaposi. Viral Proteins. Basic-Leucine Zipper Transcription Factors. Castleman Disease Early Growth Response Protein 1. Virology. Lymphoma, Primary Effusion.

Summary	Kaposi's sarcoma-associated herpesvirus (KSHV), the causative agent of three cancers: Kaposi's sarcoma (KS), primary effusion lymphoma (PEL), and the plasmablastic form of multicentric Castleman disease (MCD), shares many characteristics with other herpesviruses including the unique ability to maneuver between latent and lytic modes of infection. During a latent stage of infection, viruses remain dormant with very few genes being expressed. Alternatively, lytic infection is distinguished by the production of infectious virions and virus dissemination. In KSHV pathogenesis, the majority of infected cells support latent infections while less than 3% of them support spontaneous lytic replication. Regulation of latent and lytic infections involves interactions between virus-encoded and cellular factors. However, the exact mechanism(s) supporting it is poorly understood. In the following study, we provide evidence supporting the role for KSHV-encoded glycoprotein B (gB), a structural protein, to inhibit KSHV reactivation from latency via altering the expression of the cellular transcription factor, Early growth response-1 (Egr-1). We observed Egr-1 to specifically bind to two separate locations on the KSHV-encoded ORF50 promoter which plays a significant role in KSHV reactivation. Furthermore, we provide evidence supporting the potential mechanism for KSHV gB to regulate KSHV reactivation. These studies provide new insights and appreciation for the role of virus-encoded structural proteins to support virus latency and may contribute to the development of future therapies.
General note	Presented to the faculty of the Department of Microbiology and Immunology.
General note	Advisor: Shaw M. Akula.
General note	Title from PDF t.p. (viewed January 26, 2012).
Dissertation note	Ph.D. East Carolina University 2011.
Bibliography note	Includes bibliographical references.
Technical details	System requirements: Adobe Reader.
Technical details	Mode of access: World Wide Web.

Regulation of KSHV replication by glycoprotein B: chronicles of a virus and its host / by Ossie F. Dyson.

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