Tools

LEADER 03915nam 2200793Ia 4500

001 ocn713573454;

003 OCoLC;

005 20141212080317.0;

006 m d f ;

007 cr mn|||||||||;

008 110214s2010 mdua obt f000 0 eng d;

029 0 a| DTICEb| ADA531600;

035 a| (Sirsi) o713573454;

040 a| DTICEc| DTICEd| GPOd| UtOrBLW;

049 a| ERE#;

074 a| 0324-A-01 (online);

086 0 a| D 101.133:5357;

100 1 a| Stratis-Cullum, Dimitra N.=| ^A1412527;

245 10 a| Rapid peptide reagent isolation in a disposable microfluidic cartridge /c| Dimitra N. Stratis-Cullum, Joshua M. Kogot, and Paul M. Pellegrino.;

260 a| Adelphi, MD :b| Army Research Laboratory,c| [2010];

300 a| 1 online resource (viii, 22 pages) :b| color illustrations.;

336 a| text2| rdacontent;

337 a| computer2| rdamedia;

338 a| online resource2| rdacarrier;

490 1 a| ARL-TR ;v| 5357;

513 a| Final;b| June 2006-September 2009.;

500 a| Title from title screen (viewed on Jan. 4, 2012).;

500 a| "September 2010.";

504 a| Includes bibliographical references (p. 17-19).;

520 a| In this collaborative development project, the challenges in affinity reagent isolation were addressed by combining two seemingly disjoint technologies-biosynthetic display libraries and microfluidic systems. To isolate optimal ligands from these libraries, our project developed an automated magnetic bacterial cell sorting system, termed micromagnetic cell sorter (MMS), to screen microbial libraries with unprecedented throughput using a disposable microfluidic cartridge. We report on the sorting system performance in rare cell and ultra-rare cell recovery (populations less than 0.001%), and compare the results to the standard manual method, magnetic activated cell Sorting (MACS). To demonstrate the system effectiveness in reagent isolation through sorting against an anthrax toxin target, protective antigen was performed and comparable results to standard screening methods were obtained. In addition, the good recovery performance through MMS selection yielded a consensus sequence among 24 unique binders.;

536 b| DAAD19-03-D-004;

650 0 a| Cytologyx| Technique.=| ^A13068;

650 0 a| Peptides.=| ^A3253;

650 0 a| Biological reagents.=| ^A999610;

650 0 a| Biological weapons.=| ^A261589;

650 17 a| Chemical, biological and radiological warfare.2| scgdst;

650 27 a| Ammunition and explosives.2| scgdst;

650 17 a| Grignard reagents.2| dtict;

650 17 a| Cartridges.2| dtict;

650 17 a| Isolation.2| dtict;

650 17 a| Peptides.2| dtict;

650 27 a| Population.2| scgdst;

650 27 a| Display systems.2| scgdst;

650 27 a| Ligands.2| scgdst;

650 27 a| Chemical agents.2| scgdst;

650 27 a| Standardization.2| scgdst;

650 27 a| Sorting.2| scgdst;

650 27 a| Binders.2| scgdst;

650 27 a| Anthrax.2| scgdst;

650 27 a| Antigens.2| scgdst;

650 27 a| Biosynthesis.2| scgdst;

650 27 a| Bacterial toxins.2| scgdst;

650 27 a| Bacillus anthracis.2| scgdst;

650 27 a| Targets.2| scgdst;

650 27 a| Cells.2| scgdst;

650 27 a| Recovery.2| scgdst;

650 27 a| Magnetic fields.2| scgdst;

650 27 a| Activation.2| scgdst;

700 1 a| Kogot, Joshua M.?| UNAUTHORIZED;

700 1 a| Pellegrino, Paul M.?| UNAUTHORIZED;

710 2 a| U.S. Army Research Laboratory.=| ^A367731;

776 08 i| Print version:a| Stratis-Cullum, Dimitra N.t| Rapid peptide reagent isolation in a disposable microfluidic cartridgew| (OCoLC)770291216;

830 0 a| ARL-TR (Aberdeen Proving Ground, Md.) ;v| 5357.=| ^A566074;

856 40 u| https://purl.fdlp.gov/GPO/gpo17215;

949 a| Click on web addressw| ASISh| JOYNER94;

998 a| 2686119;

998 a| u2686119;

596 a| 1;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 2686119-1001l| JNETm| JOYNERr| Ys| Yt| JNE1DOu| 2/16/2012x| EDOCUMENTz| JERESOURCE;

Rapid peptide reagent isolation in a disposable microfluidic cartridge / Dimitra N. Stratis-Cullum, Joshua M. Kogot, and Paul M. Pellegrino.

Author/creator	Stratis-Cullum, Dimitra N.
Other author	Kogot, Joshua M.
Other author	Pellegrino, Paul M.
Other author	U.S. Army Research Laboratory.
Format	Electronic
Publication Info	Adelphi, MD : Army Research Laboratory, [2010]
Description	1 online resource (viii, 22 pages) : color illustrations.
Supplemental Content	https://purl.fdlp.gov/GPO/gpo17215
Subjects	Cytology--Technique. Peptides. Biological reagents. Biological weapons.

Series	ARL-TR ; 5357 ARL-TR (Aberdeen Proving Ground, Md.) ; 5357. ^A566074
Abstract	In this collaborative development project, the challenges in affinity reagent isolation were addressed by combining two seemingly disjoint technologies-biosynthetic display libraries and microfluidic systems. To isolate optimal ligands from these libraries, our project developed an automated magnetic bacterial cell sorting system, termed micromagnetic cell sorter (MMS), to screen microbial libraries with unprecedented throughput using a disposable microfluidic cartridge. We report on the sorting system performance in rare cell and ultra-rare cell recovery (populations less than 0.001%), and compare the results to the standard manual method, magnetic activated cell Sorting (MACS). To demonstrate the system effectiveness in reagent isolation through sorting against an anthrax toxin target, protective antigen was performed and comparable results to standard screening methods were obtained. In addition, the good recovery performance through MMS selection yielded a consensus sequence among 24 unique binders.
General note	Title from title screen (viewed on Jan. 4, 2012).
General note	"September 2010."
Bibliography note	Includes bibliographical references (p. 17-19).
Report note	Final; June 2006-September 2009.
Funding information	DAAD19-03-D-004
Issued in other form	Print version: Stratis-Cullum, Dimitra N. Rapid peptide reagent isolation in a disposable microfluidic cartridge
GPO item number	0324-A-01 (online)
Govt. docs number	D 101.133:5357

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Rapid peptide reagent isolation in a disposable microfluidic cartridge / Dimitra N. Stratis-Cullum, Joshua M. Kogot, and Paul M. Pellegrino.

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