The effects of indomethacin and dexamethasone on fibroblast chemotoxis / by John Emanuel Poulos.
| Author/creator | Poulos, John Emanuel author. |
| Other author | Kalmus, Gerhard W., degree supervisor. |
| Other author | East Carolina University. Department of Biology. |
| Format | Theses and dissertations |
| Production | 1987. |
| Description | iv, 69 leaves : illustrations ; 28 cm |
| Supplemental Content | Access via ScholarShip |
| Subjects |
| Summary | Tissue damage results in an acute inflammatory response followed by a repairative phase which is initiated by fibroblast migration towards chemoattractants released from the wound site. Boyden chambers were employed to investigate the effect of indomethacin and dexamethasone on fibroblast chemotaxis towards conditioned medium. The ability of conditioned medium to serve as a chemoattractant is possibly due to the presence of cell secreted proteins, as well as fibronectin and its fragments. Indomethacin is a non-steroidal anti-inflammatory agent that inhibits prostaglandin synthase whereas, dexamethasone inhibits phospholipase A₂. It was determined that indomethacin did not inhibit but enhanced fibroblast chemotaxis at a concentration of 1x10⁻⁴ O to 1x10⁻⁸M. This may be due to the accumulation of arachidonic acid by inhibition of prostaglandin synthase. This arachidonic acid is then free to be acted upon by 5-lipoxygenase thereby producing leukotriene B4, a potent chemoattractant for fibroblasts. Dexamethasone showed inhibition of fibroblast chemotaxis at 1x10⁻⁴M, and enhancement of chemotaxis at 1x10⁻⁸ to 1x10⁻¹⁰M. Inhibition may be due to the induction of a chemotactic regulatory protein known as lipocortin. Enhancement may be due to glucocorticoid stimulation of fibronectin synthesis and secretion, accompanied with the upregulation of fibronectin receptors. |
| General note | Submitted to the faculty of the Department of Biology. |
| General note | Advisor: Gerhard W. Kalmus |
| Dissertation note | M.S. East Carolina University 1987 |
| Bibliography note | Includes bibliographical references (leaves 57-62). |
| Genre/form | Academic theses. |
| Genre/form | Academic theses. |
| Genre/form | Thèses et écrits académiques. |
Availability
| Library | Location | Call Number | Status | Item Actions |
|---|---|---|---|---|
| Joyner | Microforms | MICROFILM* | ✔ Available | |
| Electronic Resources | Access Content Online | ✔ Available |