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001 ocn824510512;

003 OCoLC;

005 20141212050735.0;

006 m o d ;

007 cr bn|||||||||;

008 130116s2012 ncua ob 000 0 eng d;

035 a| (Sirsi) o824510512;

035 a| (OCoLC)824510512;

049 a| EREE;

090 a| QP552.P73;

100 1 a| Nagchowdhuri, Partha Sarathi.?| UNAUTHORIZED;

245 10 a| Consequences of reduced versican expression in embryonic chick synovial joint development /c| by Partha Sarathi Nagchowdhuri.;

260 a| [Greenville, N.C.] :b| East Carolina University,c| 2012.;

300 a| 72 pages :b| illustrations (color), digital, PDF file;

336 a| text2| rdacontent;

337 a| computer2| rdamedia;

338 a| online resource2| rdacarrier;

538 a| System requirements: Adobe Reader.;

538 a| Mode of access: World Wide Web.;

502 b| M.S.c| East Carolina Universityd| 2012.;

500 a| Presented to the faculty of the Department of Biology.;

500 a| Advisor: Anthony Capehart.;

500 a| Title from PDF t.p. (viewed March 5, 2013).;

520 3 a| The different mechanisms involved in vertebrate synovial joint development are actively being uncovered. A variety of studies have thus far discovered the involvement of several large molecules in the processes of joint development and cavitation. Such molecules include glycosaminoglycans, glycoproteins, proteoglycans, growth factors, and adhesive molecules. The coordinated spatiotemporal expressions of these molecules, among them chondroitin-sulfate proteoglycans (CSPG), are believed to play a crucial role in synovial joint formation. A previous study has shown that isoforms of the CSPG versican are abundantly expressed in the developing chick synovial joint during progressive stages of embryonic development (Shepard et al. 2007). The purpose of this study is to better understand the importance of versican in the developing limb joints by inhibiting its expression. We employed adenoviral-mediated RNA interference and inovo microinjection techniques to knock down endogenous versican protein levels. Results show an average reduction of 14% in the non-chondrogenic space of the joint interzone of day 10 embryos in response to versican knockdown that was coupled with attenuated expressions of key versican associating molecules such as hyaluronan, tenascin, CD44 and link protein. Impacts of versican knockdown also resulted in lowered collagen II expression among articular chondrocytes implying possible delays in chondrogenesis, or early transition toward an osteogenic fate.;

504 a| Includes bibliographical references.;

650 0 a| Proteoglycans.=| ^A14614;

650 0 a| Joints.=| ^A964458;

650 0 a| Chondrogenesis.=| ^A584581;

650 0 a| Embryology.=| ^A23;

653 a| Molecular biology;

700 1 a| Capehart, Anthony A.?| UNAUTHORIZED;

710 2 a| East Carolina University.b| Department of Biology.=| ^A637467;

856 40 z| Access via ScholarShipu| http://hdl.handle.net/10342/4090;

949 o| jgml;

994 a| C0b| ERE;

596 a| 1 4;

998 a| 3255385;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 3255385-1001l| JNETm| JOYNERr| Ys| Yt| JNE3ETDu| 1/16/2013x| ETDz| JERESOURCE;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 3255385-2001l| HSLELECm| HSLr| Ys| Yt| HEETDu| 1/16/2013x| ETDz| HERESOURCE;

Consequences of reduced versican expression in embryonic chick synovial joint development / by Partha Sarathi Nagchowdhuri.

Author/creator	Nagchowdhuri, Partha Sarathi
Other author	Capehart, Anthony A.
Other author	East Carolina University. Department of Biology.
Format	Theses and dissertations
Publication Info	[Greenville, N.C.] : East Carolina University, 2012.
Description	72 pages : illustrations (color), digital, PDF file
Supplemental Content	Access via ScholarShip
Subjects	Proteoglycans. Joints. Chondrogenesis. Embryology.

Summary	The different mechanisms involved in vertebrate synovial joint development are actively being uncovered. A variety of studies have thus far discovered the involvement of several large molecules in the processes of joint development and cavitation. Such molecules include glycosaminoglycans, glycoproteins, proteoglycans, growth factors, and adhesive molecules. The coordinated spatiotemporal expressions of these molecules, among them chondroitin-sulfate proteoglycans (CSPG), are believed to play a crucial role in synovial joint formation. A previous study has shown that isoforms of the CSPG versican are abundantly expressed in the developing chick synovial joint during progressive stages of embryonic development (Shepard et al. 2007). The purpose of this study is to better understand the importance of versican in the developing limb joints by inhibiting its expression. We employed adenoviral-mediated RNA interference and inovo microinjection techniques to knock down endogenous versican protein levels. Results show an average reduction of 14% in the non-chondrogenic space of the joint interzone of day 10 embryos in response to versican knockdown that was coupled with attenuated expressions of key versican associating molecules such as hyaluronan, tenascin, CD44 and link protein. Impacts of versican knockdown also resulted in lowered collagen II expression among articular chondrocytes implying possible delays in chondrogenesis, or early transition toward an osteogenic fate.
General note	Presented to the faculty of the Department of Biology.
General note	Advisor: Anthony Capehart.
General note	Title from PDF t.p. (viewed March 5, 2013).
Dissertation note	M.S. East Carolina University 2012.
Bibliography note	Includes bibliographical references.
Technical details	System requirements: Adobe Reader.
Technical details	Mode of access: World Wide Web.

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Consequences of reduced versican expression in embryonic chick synovial joint development / by Partha Sarathi Nagchowdhuri.

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