An investigation into the morphology of the amphibian pancreatic islet / by James Warren McLane.
| Author/creator | McLane, J. W. author. |
| Other author | Kalmus, Gerhard W., degree supervisor. |
| Other author | East Carolina University. Department of Biology. |
| Format | Theses and dissertations |
| Production | 1989. |
| Description | v leaves, 59 unnumbered leaves : illustrations ; 28 cm |
| Supplemental Content | Access via ScholarShip |
| Subjects |
| Summary | Pancreata from hindlimb bud and adult specimens were studied immunocytochemically at the light and EM levels. Antisera for glucagon, insulin, somatostatin, and pancreatic polypeptide (PP) were used to stain A-, B-, D-, and PP- cells, respectively. In the adult islets A-cells were the most numerous with some cells being extra-insular. PP-cells were numerous, and frequently PP was colocalized with glucagon. B-cells formed the core of the islets with sharp polarization of granules towards the capillaries being noted. D-cells were the least numerous cell type usually located at the periphery of the islet. Tadpole islets showed less organization. B-cells comprised most islets with other cell types being mostly extra-insular. The A-cell population of the ventral derivative appeared reduced from the population of the dorsal derivative. B-cells demonstrated the classic halo granule appearance in the adult. A-cell granules were observed to be solid and larger than B-granules with heterogeneity in size noted. D-cells contained pleomorphic granule morphology and a close association with peptidergic nerves. |
| General note | Submitted to the faculty of the Department of Biology. |
| General note | Advisor: Gerhard W. Kalmus |
| Dissertation note | M.S. East Carolina University 1989 |
| Bibliography note | Includes bibliographical references (leaves 35-38). |
| Genre/form | dissertations. |
| Genre/form | Academic theses. |
| Genre/form | Academic theses. |
| Genre/form | Thèses et écrits académiques. |