Trichomonas vaginalis-an amoeboid-adherent variant / by Joanna Simons Tysor.

Author/creator Tysor, Joanna Simons author.
Other author Bland, Charles E., degree supervisor.
Other author East Carolina University. Department of Biology.
Format Theses and dissertations
Production1987.
Descriptionviii, 71 leaves : 52 illustrations ; 28 cm
Supplemental ContentAccess via ScholarShip
Subjects

Summary Trichomonas vaginalis, first described by Donne in 1836, is a human, urogenital, sexually transmitted, protozoan parasite. Although usually ovoid-to-ellipsoidal and active, amoeboid-adherent forms have been described that are nonmotile, have short cytoplasmic pseudopodia, have missing or sluggish flagella, and have a tendency to stick to adjacent surfaces. Through a cloning technique, amoeboid-adherent strains that remain stable through subculturing tuning have been isolated. In this study, the ovoid-motile strain is compared to the amoeboid-adherent strain via light and electron microscopy, analysis of surface proteins, comparison of colonial morphology, analysis of growth rates, and lactic acid production. Except for cell shape the ovoid-motile and amoeboid-adherent forms were found to exhibit similarities in organelle structure and arrangement. Their colonial morphology differs in that the amoeboid colonies adhere to each other to form pinpoint colonies while the motile forms demonstrate diffuse growth throughout the culture tube. The banding from protein analysis varied slightly suggesting a possible difference in the surface proteins.
General noteSubmitted to the faculty of the Department of Biology.
General noteAdvisor: Charles E. Bland
Dissertation noteM.S. East Carolina University 1987
Bibliography noteIncludes bibliographical references (leaves 67-71).
Genre/formdissertations.
Genre/formAcademic theses.
Genre/formAcademic theses.
Genre/formThèses et écrits académiques.

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