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003 OCoLC;

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006 m o d ;

007 cr bn|||||||||;

008 130612s2013 ncua obm 000 0 eng d;

035 a| (Sirsi) o847755845;

035 a| (OCoLC)847755845;

049 a| EREE;

090 a| RC271.A62;

100 1 a| Sattler, Jennifer A.?| UNAUTHORIZED;

245 10 a| Comparison of two human cell lines following exposure to low dose radiation and treatment with soybean miso and its active ingredient genistein /c| by Jennifer A. Sattler.;

260 a| [Greenville, N.C.] :b| East Carolina University,c| 2013.;

300 a| 116 pages :b| ills. (some color), digital, PDF file;

336 a| textb| txt2| rdacontent;

337 a| computerb| c2| rdamedia;

338 a| online resourceb| cr2| rdacarrier;

538 a| System requirements: Adobe Reader.;

538 a| Mode of access: World Wide Web.;

502 b| Ph. D.c| East Carolina Universityd| 2013.;

500 a| Presented to the faculty of the Department of Physics.;

500 a| Advisors: C. Ruth Kempf and Roberta Johnke.;

500 a| Title from PDF t.p. (viewed July 2, 2013).;

520 3 a| Exposure to radiation is increasingly becoming a worldwide health issue. Because of this, the need for effective radioprotectors is critical. This need is not met by amifostine, the current "gold standard" in radioprotectors, due to its limited availability and potentially severe side effects. The fermented soy product miso has been credited with a host of beneficial effects, among which are radioprotection, and, as a common food, miso is readily available, known to be safe and is easily administered. As such, it is the hypothesis of this dissertation that miso may be an effective radioprotector. Unfortunately, few rigorous studies of miso's reputed actions have been undertaken. It is the objective of this investigation, therefore, to better identify and characterize the radioprotective and antioncogenic properties of miso and its most abundant active ingredient genistein. To accomplish this, experiments were designed using tumorigenic (PC3 cells) and non-tumorigenic (RWPE-1 cells) experimental prostate models to characterize the effects of miso and genistein both alone and following a series of low dose radiation exposures. Results demonstrated that neither miso nor genistein appeared to display radioprotective abilities in the radiation dose range used in this investigation. However, both compounds induced a strong growth inhibitory effect that appeared to synergize with radiation to significantly reduce cell survival in a manner that preferentially enhanced tumor cell kill over non-tumor cell kill, suggesting potential antioncogenic potential for these compounds.;

504 a| Includes bibliographical references.;

650 0 a| Cancerx| Alternative treatment.=| ^A476952;

650 0 a| Radiation-protective agents.=| ^A680272;

650 0 a| Miso.=| ^A1167030;

650 0 a| Genistein.=| ^A1167390;

650 0 a| Low-level radiation.=| ^A378295;

653 a| Alternative medicine;

653 a| Cellular biology;

653 a| Physics;

700 1 a| Kempf, C. Ruth.?| UNAUTHORIZED;

700 1 a| Johnke, Roberta M.?| UNAUTHORIZED;

710 2 a| East Carolina University.b| Department of Physics.?| UNAUTHORIZED;

856 40 z| Access via ScholarShipu| http://hdl.handle.net/10342/1820;

949 o| jgml;

994 a| C0b| ERE;

596 a| 1 4;

998 a| 3321535;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 3321535-1001l| JNETm| JOYNERr| Ys| Yt| JNE3ETDu| 6/12/2013x| ETDz| JERESOURCE;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 3321535-2001l| HSLELECm| HSLr| Ys| Yt| HEETDu| 6/12/2013x| ETDz| HERESOURCE;

Comparison of two human cell lines following exposure to low dose radiation and treatment with soybean miso and its active ingredient genistein / by Jennifer A. Sattler.

Author/creator	Sattler, Jennifer A.
Other author	Kempf, C. Ruth.
Other author	Johnke, Roberta M.
Other author	East Carolina University. Department of Physics.
Format	Theses and dissertations
Publication Info	[Greenville, N.C.] : East Carolina University, 2013.
Description	116 pages : ills. (some color), digital, PDF file
Supplemental Content	Access via ScholarShip
Subjects	Cancer--Alternative treatment. Radiation-protective agents. Miso. Genistein. Low-level radiation.

Summary	Exposure to radiation is increasingly becoming a worldwide health issue. Because of this, the need for effective radioprotectors is critical. This need is not met by amifostine, the current "gold standard" in radioprotectors, due to its limited availability and potentially severe side effects. The fermented soy product miso has been credited with a host of beneficial effects, among which are radioprotection, and, as a common food, miso is readily available, known to be safe and is easily administered. As such, it is the hypothesis of this dissertation that miso may be an effective radioprotector. Unfortunately, few rigorous studies of miso's reputed actions have been undertaken. It is the objective of this investigation, therefore, to better identify and characterize the radioprotective and antioncogenic properties of miso and its most abundant active ingredient genistein. To accomplish this, experiments were designed using tumorigenic (PC3 cells) and non-tumorigenic (RWPE-1 cells) experimental prostate models to characterize the effects of miso and genistein both alone and following a series of low dose radiation exposures. Results demonstrated that neither miso nor genistein appeared to display radioprotective abilities in the radiation dose range used in this investigation. However, both compounds induced a strong growth inhibitory effect that appeared to synergize with radiation to significantly reduce cell survival in a manner that preferentially enhanced tumor cell kill over non-tumor cell kill, suggesting potential antioncogenic potential for these compounds.
General note	Presented to the faculty of the Department of Physics.
General note	Advisors: C. Ruth Kempf and Roberta Johnke.
General note	Title from PDF t.p. (viewed July 2, 2013).
Dissertation note	Ph. D. East Carolina University 2013.
Bibliography note	Includes bibliographical references.
Technical details	System requirements: Adobe Reader.
Technical details	Mode of access: World Wide Web.

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Comparison of two human cell lines following exposure to low dose radiation and treatment with soybean miso and its active ingredient genistein / by Jennifer A. Sattler.

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