Generation and characterization of a unique T200 molecule in rhesus monkeys / by Lorita Maria Rebellato.
| Author/creator | Rebellato, Lorita Maria author. |
| Other author | Thomas, Judith M., degree supervisor. |
| Other author | East Carolina University. Department of Biology. |
| Format | Theses and dissertations |
| Production | 1988. |
| Description | viii, 121 leaves : illustrations ; 28 cm |
| Supplemental Content | Access via ScholarShip |
| Subjects |
| Variant title | Abstract title: Generation and characterization of a MoAb against a unique T200 molecule in rhesus monkeys |
| Subject | The leukocyte common antigen (T200), now termed CD45, is present on all leukocytes. T200 molecule was first found in the rat using a heterologous antiserum, has also been identified in humans and mice, and the human form has been designated CD45. Biochemical analysis indicates that at least four polypeptides carry epitopes identified by many monoclonal antibodies (moAbs). Some antibodies, now termed CD45R, were found to precipitate only the two higher molecular weight polypeptides. These antibodies were found to react predominantly with B lymphocytes. Recently, it has been determined that these B restricted or CD45R moAbs recognize a set of T cells. This study describes the generation, characterization, tissue distribution, and some functional properties of anti-7H5, a moAb which appears to recognize a unique T200 molecule on rhesus monkey mononuclear leukocytes. The role of this molecule on the alternative pathway of activation of T lymphocytes, and the enhancing effect of anti-7H5 on ConA cultures remains to be elucidated. The results of these studies suggest 7H5 molecule may function in association with other cytoplasmic and/or cell surface molecules to influence the activation of T lymphocyte function. This common leukocyte antigen has a restricted distribution, similar to that exhibited by CD45R molecules in rats and humans. However, the 7H5 determinant is antigenically distinct from the CD45R determinant defined by anti-2H4 moAb. Furthermore, these molecules show differences in two bands in SDS-PAGE gels. The 7H5 molecule exhibits a unique very high mw band not seen in other descriptions of CD45R molecules. 7H5 also is unique from other CD45R molecules in that binding of anti-7H5 does not block NK lysis, and it selectively potentiates ConA, but not PHA responses. Collectively, these results suggest 7H5 is a unique variant of a CD45R molecule in rhesus monkeys and may play a central role in the function of lymphoid cells, perhaps in association with both cytoplasmic molecules and others at the cell surface. |
| General note | Submitted to the faculty of the Department of Biology. |
| General note | Advisor: Judith M. Thomas |
| Dissertation note | M.S. East Carolina University 1988 |
| Bibliography note | Includes bibliographical references (leaves 114-121). |
| Genre/form | Academic theses. |
| Genre/form | Academic theses. |
| Genre/form | Thèses et écrits académiques. |