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035 a| (Sirsi) o889804704;

035 a| (OCoLC)889804704;

043 a| u-at---a| a-io---;

049 a| EREE;

090 a| QR184.315;

100 1 a| Newhouse, Daniel J,e| author.?| UNAUTHORIZED;

245 10 a| MHC class I variation among wild, domestic and insular populations of the zebra finch (Taeniopygia guttata) /c| by Daniel J Newhouse.;

264 1 a| [Greenville, N.C.] :b| [East Carolina University],c| 2014.;

300 a| 47 pages :b| color illustrations;

336 a| textb| txt2| rdacontent;

337 a| computerb| c2| rdamedia;

338 a| online resourceb| cr2| rdacarrier;

347 a| text fileb| PDFc| 2.78Mb2| rda;

538 a| System requirements: Adobe Reader.;

538 a| Mode of access: World Wide Web.;

502 b| M.S.c| East Carolina Universityd| 2014.;

500 a| Presented to the faculty of the Department of Biology.;

500 a| Advisor: Christopher Balakrishnan.;

500 a| Title from PDF t.p. (viewed September 15, 2014).;

520 3 a| The zebra finch (Taeniopygia guttata) is a passerine bird native to Australia and the Lesser Sunda Islands, with domesticated populations derived from each of these localities. The Lesser Sundas and domesticated zebra finches have undergone historic bottlenecks and previous analyses using neutral markers have reported striking genetic differences among these populations. However, comparisons utilizing functional loci are lacking. Immune genes are thought to strongly rely on genetic variation to convey a greater resistance to pathogens. Genes of the major histocompatibility complex (MHC), a component of the vertebrate adaptive immune response involved in antigen presentation, have received considerable attention as a classic example of balancing selection. Here, we characterize the third exon of the highly variable MHC class I [alpha] chain, which encodes the [alpha]2 domain of the peptide binding region (PBR). Despite natural and human-mediated population bottlenecks, we find that high MHC polymorphism persists across all populations. We report higher levels of nucleotide diversity when compared with neutral loci and strong evidence of positive selection acting on important residues forming the PBR. Clear population differentiation is also evident and this may be due to adaptation to captivity and life on islands. Due to its importance in pathogen recognition, selection seems to have maintained variability of the MHC class I [alpha]2 domain, even in the presence of a genetic bottleneck.;

504 a| Includes bibliographical references.;

650 0 a| Major histocompatibility complexx| Genetic aspects.=| ^A476364;

650 0 a| Zebra finchz| Australia.=| ^A855438;

650 0 a| Zebra finchz| Lesser Sunda Islands.=| ^A855438;

653 a| Biology;

653 a| Genetics;

700 1 a| Balakrishnan, Chris,e| degree supervisor.=| ^A1478344;

710 2 a| East Carolina University.b| Department of Biology.=| ^A637467;

856 40 z| Access via ScholarShipu| http://hdl.handle.net/10342/4549;

949 o| jgml;

994 a| C0b| ERE;

596 a| 1 4;

998 a| 3510201;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 3510201-1001l| JNETm| JOYNERr| Ys| Yt| JNE3ETDu| 9/2/2014x| ETDz| JERESOURCE;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 3510201-2001l| HSLELECm| HSLr| Ys| Yt| HEETDu| 9/2/2014x| ETDz| HERESOURCE;

MHC class I variation among wild, domestic and insular populations of the zebra finch (Taeniopygia guttata) / by Daniel J Newhouse.

Author/creator	Newhouse, Daniel J author.
Other author	Balakrishnan, Chris, degree supervisor.
Other author	East Carolina University. Department of Biology.
Format	Theses and dissertations
Publication	[Greenville, N.C.] : [East Carolina University], 2014.
Description	47 pages : color illustrations
Supplemental Content	Access via ScholarShip
Subjects	Major histocompatibility complex--Genetic aspects. Zebra finch--Australia. Zebra finch--Lesser Sunda Islands.

Summary	The zebra finch (Taeniopygia guttata) is a passerine bird native to Australia and the Lesser Sunda Islands, with domesticated populations derived from each of these localities. The Lesser Sundas and domesticated zebra finches have undergone historic bottlenecks and previous analyses using neutral markers have reported striking genetic differences among these populations. However, comparisons utilizing functional loci are lacking. Immune genes are thought to strongly rely on genetic variation to convey a greater resistance to pathogens. Genes of the major histocompatibility complex (MHC), a component of the vertebrate adaptive immune response involved in antigen presentation, have received considerable attention as a classic example of balancing selection. Here, we characterize the third exon of the highly variable MHC class I [alpha] chain, which encodes the [alpha]2 domain of the peptide binding region (PBR). Despite natural and human-mediated population bottlenecks, we find that high MHC polymorphism persists across all populations. We report higher levels of nucleotide diversity when compared with neutral loci and strong evidence of positive selection acting on important residues forming the PBR. Clear population differentiation is also evident and this may be due to adaptation to captivity and life on islands. Due to its importance in pathogen recognition, selection seems to have maintained variability of the MHC class I [alpha]2 domain, even in the presence of a genetic bottleneck.
General note	Presented to the faculty of the Department of Biology.
General note	Advisor: Christopher Balakrishnan.
General note	Title from PDF t.p. (viewed September 15, 2014).
Dissertation note	M.S. East Carolina University 2014.
Bibliography note	Includes bibliographical references.
Technical details	System requirements: Adobe Reader.
Technical details	Mode of access: World Wide Web.

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MHC class I variation among wild, domestic and insular populations of the zebra finch (Taeniopygia guttata) / by Daniel J Newhouse.

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