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LEADER 03367cam 2200529Ii 4500

001 ocn889804739;

003 OCoLC;

005 20141212071935.0;

006 m o d ;

007 cr bn|||||||||;

008 140902s2014 ncua ob 000 0 eng d;

035 a| (Sirsi) o889804739;

035 a| (OCoLC)889804739;

049 a| EREE;

090 a| QL537.D76;

100 1 a| Rummings, Wayne Anthony,c| Jr.,e| author.?| UNAUTHORIZED;

245 10 a| Investigating the interaction of RecQL4 and Mcm10 in Drosophila melanogaster /c| by Wayne Anthony Rummings, Jr.;

264 1 a| [Greenville, N.C.] :b| [East Carolina University],c| 2014.;

300 a| 60 pages :b| illustrations (some color);

336 a| textb| txt2| rdacontent;

337 a| computerb| c2| rdamedia;

338 a| online resourceb| cr2| rdacarrier;

347 a| text fileb| PDFc| 0.94Mb2| rda;

538 a| System requirements: Adobe Reader.;

538 a| Mode of access: World Wide Web.;

502 b| M.S.c| East Carolina Universityd| 2014.;

500 a| Presented to the faculty of the Department of Biology.;

500 a| Advisor: Tim W. Christensen.;

500 a| Title from PDF t.p. (viewed October 2, 2014).;

520 3 a| Accurate duplication and regulation of the eukaryotic genome requires precise coordination among multiple replication factors. RecQL4, the least characterized RecQ protein, is a 1208 amino acid protein containing a centrally located and highly conserved helicase domain. Mutations in RecQL4 may lead to distinct clinical diseases with premature aging symptoms and increased cancer rates. Recently, efforts have focused on elucidating specific protein-protein interactions to shed light into the cellular processes in which RecQL4 may be involved. Previously, others have shown a direct interaction between RecQL4 and Mini-chromosome-maintenance protein 10 (Mcm10), a highly conserved protein with essential roles in DNA replication and heterochromatin formation. Work in 293T cells suggests that Mcm10 mediates the association of RecQL4 with the Mcm2-7 helicase; however, the biological ramifications of this interaction in a multicellular context remain unclear. Using both yeast two-hybrid and genetic approaches, we have shown that the RecQL4-Mcm10 interaction is conserved in Drosophila melanogaster and is important for alternate modes of replication. This study highlights the key roles of RecQL4 and Mcm10 in cell viability and further demonstrates their cooperative function in DNA replication.;

504 a| Includes bibliographical references.;

650 0 a| Drosophila melanogaster.=| ^A86209;

650 0 a| Eukaryotic cellsx| Genetics.=| ^A1244232;

650 0 a| Genetic regulation.=| ^A6251;

650 0 a| Amino acids.=| ^A6734;

650 0 a| Mutation (Biology)=| ^A2390;

653 a| Molecular biology;

700 1 a| Christensen, Tim,e| degree supervisor.=| ^A1389912;

710 2 a| East Carolina University.b| Department of Biology.=| ^A637467;

856 40 z| Access via ScholarShipu| http://hdl.handle.net/10342/4551;

949 o| jgml;

994 a| C0b| ERE;

596 a| 1 4;

998 a| 3510210;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 3510210-1001l| JNETm| JOYNERr| Ys| Yt| JNE3ETDu| 9/2/2014x| ETDz| JERESOURCE;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 3510210-2001l| HSLELECm| HSLr| Ys| Yt| HEETDu| 9/2/2014x| ETDz| HERESOURCE;

Investigating the interaction of RecQL4 and Mcm10 in Drosophila melanogaster / by Wayne Anthony Rummings, Jr.

Author/creator	Rummings, Wayne Anthony author.
Other author	Christensen, Tim, degree supervisor.
Other author	East Carolina University. Department of Biology.
Format	Theses and dissertations
Publication	[Greenville, N.C.] : [East Carolina University], 2014.
Description	60 pages : illustrations (some color)
Supplemental Content	Access via ScholarShip
Subjects	Drosophila melanogaster. Eukaryotic cells--Genetics. Genetic regulation. Amino acids. Mutation (Biology)

Summary	Accurate duplication and regulation of the eukaryotic genome requires precise coordination among multiple replication factors. RecQL4, the least characterized RecQ protein, is a 1208 amino acid protein containing a centrally located and highly conserved helicase domain. Mutations in RecQL4 may lead to distinct clinical diseases with premature aging symptoms and increased cancer rates. Recently, efforts have focused on elucidating specific protein-protein interactions to shed light into the cellular processes in which RecQL4 may be involved. Previously, others have shown a direct interaction between RecQL4 and Mini-chromosome-maintenance protein 10 (Mcm10), a highly conserved protein with essential roles in DNA replication and heterochromatin formation. Work in 293T cells suggests that Mcm10 mediates the association of RecQL4 with the Mcm2-7 helicase; however, the biological ramifications of this interaction in a multicellular context remain unclear. Using both yeast two-hybrid and genetic approaches, we have shown that the RecQL4-Mcm10 interaction is conserved in Drosophila melanogaster and is important for alternate modes of replication. This study highlights the key roles of RecQL4 and Mcm10 in cell viability and further demonstrates their cooperative function in DNA replication.
General note	Presented to the faculty of the Department of Biology.
General note	Advisor: Tim W. Christensen.
General note	Title from PDF t.p. (viewed October 2, 2014).
Dissertation note	M.S. East Carolina University 2014.
Bibliography note	Includes bibliographical references.
Technical details	System requirements: Adobe Reader.
Technical details	Mode of access: World Wide Web.

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Investigating the interaction of RecQL4 and Mcm10 in Drosophila melanogaster / by Wayne Anthony Rummings, Jr.

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