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035 a| (Sirsi) o902728170;

035 a| (OCoLC)902728170;

049 a| EREE;

100 1 a| Martinson, David A.,e| author.?| UNAUTHORIZED;

245 14 a| The iron response regulator Irr controls iron homoeostasis in Brucella /c| by David A. Martinson.;

264 1 a| [Greenville, N.C.] :b| [East Carolina University],c| 2014.;

300 a| 242 pages :b| illustrations (some color);

336 a| textb| txt2| rdacontent;

337 a| computerb| c2| rdamedia;

338 a| online resourceb| cr2| rdacarrier;

347 a| text fileb| PDFc| 2.66Mb2| rda;

490 1 a| ECU Brody School of Medicine thesis;

538 a| System requirements: Adobe Reader.;

538 a| Mode of access: World Wide Web.;

502 b| Ph.D.c| East Carolina Universityd| 2014.;

500 a| Presented to the Graduate Faculty of the Brody School of Medicine Department of Microbiology and Immunology.;

500 a| Advisor: R. Martin Roop, II.;

500 a| Title from PDF t.p. (viewed February 04, 2015).;

520 3 a| Members of the genus Brucella are small, Gram-negative intracellular bacterial pathogens that are capable of infecting a wide range of mammalian hosts including humans. Brucella primarily reside inside of host macrophages. As an intracellular pathogen, Brucella must overcome iron sequestration in the host cell by utilizing highly efficient iron transport systems. These systems must be tightly regulated, however, as excess intracellular iron is toxic to the bacterial cells. Most of the alpha-proteobacteria rely on a transcriptional regulator called the iron response regulator (Irr) to control the expression of their iron metabolism genes. The work presented in this dissertation provides evidence to support the proposition that the Irr protein is the main iron-responsive transcriptional regulator in Brucella. Irr serves as an activator of genes coding for products that are involved in iron acquisition and a repressor of genes for products that require high levels of iron for their function, or serve as iron export and storage proteins when cellular iron levels are low. Irr is a conditionally stable protein that is present when cellular iron levels are low, and is degraded when cellular iron levels are high. Irr activity is controlled by inactivation and degradation through its interaction with heme, which is synthesized when cellular iron levels rise. Brucella has another iron-responsive regulator that is also found in some members of the alpha-proteobacteria called the rhizobial iron regulator (RirA). RirA is active when cellular iron levels are high, and its regulon partially overlaps with that of Irr in Brucella. The activity of Irr when cellular iron levels are low, and the activity of RirA when cellular iron levels are high, ensures that cellular iron levels are maintained at physiological levels, protecting against iron starvation, and against iron related toxicity in Brucella.;

504 a| Includes bibliographical references.;

650 2 a| Gram-Negative Bacterial Infections.=| ^A931426;

650 2 a| Bacterial Proteinsx| metabolism.=| ^A938874;

650 2 a| Brucella abortusx| metabolism.=| ^A1260864;

650 2 a| Gene Expression Regulation, Bacterial.=| ^A926528;

650 2 a| Transcription Factorsx| metabolism.=| ^A944052;

653 a| Microbiology;

700 1 a| Roop, R. Martin,c| II,e| degree supervisor.=| ^A1383281;

710 2 a| East Carolina University.b| Department of Microbiology and Immunology.?| UNAUTHORIZED;

830 0 a| ECU Brody School of Medicine thesis.=| ^A964744;

856 40 z| Access via ScholarShipu| http://hdl.handle.net/10342/4659;

949 a| Click on web addressw| ASISh| JOYNER101;

949 a| Click on web addressw| ASISh| HSL111;

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994 a| C0b| ERE;

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596 a| 1 4;

998 a| 3710503;

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The iron response regulator Irr controls iron homoeostasis in Brucella / by David A. Martinson.

Author/creator	Martinson, David A. author.
Other author	Roop, R. Martin, II, degree supervisor.
Other author	East Carolina University. Department of Microbiology and Immunology.
Format	Theses and dissertations
Publication	[Greenville, N.C.] : [East Carolina University], 2014.
Description	242 pages : illustrations (some color)
Supplemental Content	Access via ScholarShip
Subjects	Gram-Negative Bacterial Infections. Bacterial Proteins--metabolism. Brucella abortus--metabolism. Gene Expression Regulation, Bacterial. Transcription Factors--metabolism.

Series	ECU Brody School of Medicine thesis ECU Brody School of Medicine thesis. ^A964744
Summary	Members of the genus Brucella are small, Gram-negative intracellular bacterial pathogens that are capable of infecting a wide range of mammalian hosts including humans. Brucella primarily reside inside of host macrophages. As an intracellular pathogen, Brucella must overcome iron sequestration in the host cell by utilizing highly efficient iron transport systems. These systems must be tightly regulated, however, as excess intracellular iron is toxic to the bacterial cells. Most of the alpha-proteobacteria rely on a transcriptional regulator called the iron response regulator (Irr) to control the expression of their iron metabolism genes. The work presented in this dissertation provides evidence to support the proposition that the Irr protein is the main iron-responsive transcriptional regulator in Brucella. Irr serves as an activator of genes coding for products that are involved in iron acquisition and a repressor of genes for products that require high levels of iron for their function, or serve as iron export and storage proteins when cellular iron levels are low. Irr is a conditionally stable protein that is present when cellular iron levels are low, and is degraded when cellular iron levels are high. Irr activity is controlled by inactivation and degradation through its interaction with heme, which is synthesized when cellular iron levels rise. Brucella has another iron-responsive regulator that is also found in some members of the alpha-proteobacteria called the rhizobial iron regulator (RirA). RirA is active when cellular iron levels are high, and its regulon partially overlaps with that of Irr in Brucella. The activity of Irr when cellular iron levels are low, and the activity of RirA when cellular iron levels are high, ensures that cellular iron levels are maintained at physiological levels, protecting against iron starvation, and against iron related toxicity in Brucella.
General note	Presented to the Graduate Faculty of the Brody School of Medicine Department of Microbiology and Immunology.
General note	Advisor: R. Martin Roop, II.
General note	Title from PDF t.p. (viewed February 04, 2015).
Dissertation note	Ph.D. East Carolina University 2014.
Bibliography note	Includes bibliographical references.
Technical details	System requirements: Adobe Reader.
Technical details	Mode of access: World Wide Web.

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