Tools

LEADER 04852nam 2200565Ii 4500

001 ocn902728089;

003 OCoLC;

005 20150428112225.0;

006 m o d ;

007 cr bn|||||||||;

008 150204s2014 ncua ob 000 0 eng d;

035 a| (Sirsi) o902728089;

035 a| (OCoLC)902728089;

040 a| EREe| rdac| EREd| UtOrBLW;

049 a| EREE;

100 1 a| McPeek, Matthew K.,e| author.?| UNAUTHORIZED;

245 10 a| Expression of microRNA in alveolar macrophages deficient in PPARy /c| by Matthew McPeek.;

264 1 a| [Greenville, N.C.] :b| [East Carolina University],c| 2014.;

300 a| 72 pages :b| illustrations (some color);

336 a| textb| txt2| rdacontent;

337 a| computerb| c2| rdamedia;

338 a| online resourceb| cr2| rdacarrier;

347 a| text fileb| PDFc| 0.98Mb2| rda;

490 1 a| ECU Brody School of Medicine thesis;

538 a| System requirements: Adobe Reader.;

538 a| Mode of access: World Wide Web.;

502 b| M.S.c| East Carolina Universityd| 2014.;

500 a| Presented to the faculty of the Brody School of Medicine.;

500 a| Advisor: Mary Jane Thomassen.;

500 a| Title from PDF t.p. (viewed February 04, 2015).;

520 3 a| The nuclear transcription factor Peroxisome proliferator-activated receptor gamma (PPAR[gamma]) is a negative regulator of macrophage activation and inflammatory mediators. Alveolar macrophages of healthy individuals constitutively express PPAR[gamma]. Decreased activity and expression of PPAR[gamma] are observed in the alveolar macrophages from patients suffering from inflammatory conditions such as pulmonary alveolar proteinosis (PAP) and sarcoidosis. These finding suggest that PPAR[gamma] activity may have an integral role in maintaining lung homeostasis. This study tested the hypothesis that microRNA expression would be dysregulated in murine alveolar macrophages deficient in PPAR[gamma]. microRNA (miR) are small non-coding RNA molecules that post-transcriptionally regulate the expression of messenger RNA. Evaluation of microRNA in the murine model of PAP, the GM-CSF-KO mouse, demonstrates the elevation of miR-27a and miR-27b which target PPAR[gamma]. The deficiency of PPAR[gamma] and the lipid transporters ABCA1 and ABCG1 have been shown to contribute to the pathology of PAP. The microRNA miR-33-3p and miR-33-5p, which target these lipid transporters, were also elevated in GM-CSF-KO mice. Pulmonary granulomas comparable to those observed in pulmonary sarcoidosis are induced by instillation of multiwall carbon nanotubes (MWCNT) in C57Bl/6 mice. These animals have decreased PPAR[gamma] activity and show elevated expression of miR-27a and miR-27b. It was also observed that the expression of the transporters ABCA1 and ABCG1 were decreased in MWCNT instilled mice. Expression of miR-33-3p and miR-33-5p was elevated in MWCNT instilled animals. The expression of microRNA that affects the activity of NF-[kappa]B is also elevated in both murine models. We next investigated the use of PPAR[gamma] agonist rosiglitazone on the expression of microRNA and messenger RNA. The use of rosiglitazone altered the expression of microRNA in both GM-CSF-KO and C57Bl/6+MWCNT mice. Rosiglitazone treatment altered the expression of the lipid transporter ABCA1and ABCG1 in C57Bl/6+MWCNT mice. The elevation of proinflammatory cytokines was also observed. Taken together, these observations support the hypothesis that PPARgamma activity effects the microRNA and gene expression in alveolar macrophages which is critical to overall lung homeostasis. Understanding the relationship between PPAR[gamma] and microRNA in alveolar macrophage biology will provide insight into the regulation of the lung environment and possible therapeutic targets.;

504 a| Includes bibliographical references.;

650 2 a| Pulmonary Alveolar Proteinosisx| metabolism.=| ^A1260920;

650 2 a| Sarcoidosisx| metabolism.=| ^A1260928;

650 2 a| Macrophages, Alveolar.=| ^A1204586;

650 2 a| MicroRNAsx| metabolism.=| ^A1260988;

653 a| Biology;

700 1 a| Thomassen, Mary Jane,e| degree supervisor.?| UNAUTHORIZED;

710 2 a| Brody School of Medicine.b| Office of Research and Graduate Studies.b| Biomedical Sciences.?| UNAUTHORIZED;

830 0 a| ECU Brody School of Medicine thesis.=| ^A964744;

856 40 z| Access via ScholarShipu| http://hdl.handle.net/10342/4664;

949 a| Click on web addressw| ASISh| JOYNER101;

949 a| Click on web addressw| ASISh| HSL111;

949 o| jdjr;

994 a| C0b| ERE;

096 a| WF 600;

596 a| 1 4;

998 a| 3710504;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 3710504-1001l| JNETm| JOYNERr| Ys| Yt| JNE3ETDu| 2/4/2015x| ETDz| JERESOURCE;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 3710504-2001l| HSLELECm| HSLr| Ys| Yt| HEETDu| 2/4/2015x| ETDz| HERESOURCE;

Expression of microRNA in alveolar macrophages deficient in PPARy / by Matthew McPeek.

Author/creator	McPeek, Matthew K. author.
Other author	Thomassen, Mary Jane, degree supervisor.
Other author	Brody School of Medicine. Office of Research and Graduate Studies. Biomedical Sciences.
Format	Theses and dissertations
Publication	[Greenville, N.C.] : [East Carolina University], 2014.
Description	72 pages : illustrations (some color)
Supplemental Content	Access via ScholarShip
Subjects	Pulmonary Alveolar Proteinosis--metabolism. Sarcoidosis--metabolism. Macrophages, Alveolar. MicroRNAs--metabolism.

Series	ECU Brody School of Medicine thesis ECU Brody School of Medicine thesis. ^A964744
Summary	The nuclear transcription factor Peroxisome proliferator-activated receptor gamma (PPAR[gamma]) is a negative regulator of macrophage activation and inflammatory mediators. Alveolar macrophages of healthy individuals constitutively express PPAR[gamma]. Decreased activity and expression of PPAR[gamma] are observed in the alveolar macrophages from patients suffering from inflammatory conditions such as pulmonary alveolar proteinosis (PAP) and sarcoidosis. These finding suggest that PPAR[gamma] activity may have an integral role in maintaining lung homeostasis. This study tested the hypothesis that microRNA expression would be dysregulated in murine alveolar macrophages deficient in PPAR[gamma]. microRNA (miR) are small non-coding RNA molecules that post-transcriptionally regulate the expression of messenger RNA. Evaluation of microRNA in the murine model of PAP, the GM-CSF-KO mouse, demonstrates the elevation of miR-27a and miR-27b which target PPAR[gamma]. The deficiency of PPAR[gamma] and the lipid transporters ABCA1 and ABCG1 have been shown to contribute to the pathology of PAP. The microRNA miR-33-3p and miR-33-5p, which target these lipid transporters, were also elevated in GM-CSF-KO mice. Pulmonary granulomas comparable to those observed in pulmonary sarcoidosis are induced by instillation of multiwall carbon nanotubes (MWCNT) in C57Bl/6 mice. These animals have decreased PPAR[gamma] activity and show elevated expression of miR-27a and miR-27b. It was also observed that the expression of the transporters ABCA1 and ABCG1 were decreased in MWCNT instilled mice. Expression of miR-33-3p and miR-33-5p was elevated in MWCNT instilled animals. The expression of microRNA that affects the activity of NF-[kappa]B is also elevated in both murine models. We next investigated the use of PPAR[gamma] agonist rosiglitazone on the expression of microRNA and messenger RNA. The use of rosiglitazone altered the expression of microRNA in both GM-CSF-KO and C57Bl/6+MWCNT mice. Rosiglitazone treatment altered the expression of the lipid transporter ABCA1and ABCG1 in C57Bl/6+MWCNT mice. The elevation of proinflammatory cytokines was also observed. Taken together, these observations support the hypothesis that PPARgamma activity effects the microRNA and gene expression in alveolar macrophages which is critical to overall lung homeostasis. Understanding the relationship between PPAR[gamma] and microRNA in alveolar macrophage biology will provide insight into the regulation of the lung environment and possible therapeutic targets.
General note	Presented to the faculty of the Brody School of Medicine.
General note	Advisor: Mary Jane Thomassen.
General note	Title from PDF t.p. (viewed February 04, 2015).
Dissertation note	M.S. East Carolina University 2014.
Bibliography note	Includes bibliographical references.
Technical details	System requirements: Adobe Reader.
Technical details	Mode of access: World Wide Web.

Availability

Library	Location	Call Number	Status	Item Actions
	Electronic Resources	Access Content Online	✔ Available

Expression of microRNA in alveolar macrophages deficient in PPARy / by Matthew McPeek.

Click here for more information about this title

Availability