Allograft rejection of cultured mouse heart cultivated in media containing chlorpromazine prior to transplantation / by Don Thomas Peacock, Jr.
| Author/creator | Peacock, Don Thomas author. |
| Other author | Kalmus, Gerhard W., degree supervisor. |
| Other author | East Carolina University. Department of Biology. |
| Format | Theses and dissertations |
| Production | 1990. |
| Description | v, 43 leaves : illustrations ; 28 cm |
| Supplemental Content | Access via ScholarShip |
| Subjects |
| Subject | A tissue culture protocol was developed for neonatal mouse cardiac tissue to evaluate the effect of Chlorpromazine on allograft rejection. Hearts were removed from C57BI/6 neonate mice (24hrs. postpartum) and the ventricles cultured in media 199, supplemented with L-glutamine (0.1 g/L), 20% fetal bovine serum and gentamycin (10ml/liter of a 10mg/liter concentration). Experimental cultures were treated with 0.05 mg/ml chlorpromazine (CPZ) and the cultured cardiac tissue was grafted subcutaneously to the ear of adult C3H mice. Cardiac function of the allografts was monitored by observing pulsation under the skin. Transplants were monitored daily (every 24 hrs.) and cessation of pulsing graft was recorded as rejection time. Grafts not showing pulsation were considered non-viable and a 0 day rejection time was recorded. These unsuccessful! transplants were noted and a percent of viable grafts recorded. Seven days post transplantation a number of grafts in each group were removed and histologically examined . Results showed less necrosis of tissue in groups treated with CPZ prior to transplantation. Histological examination was also done on non-transplanted cardiac tissue cultured for 7 days with or without 0.05mg/ml CPZ. In these experiments CPZ had a stabilizing effect on the cell membrane which is in agreement with previous reports that CPZ maintains the cell membrane integrity in vitro (Kalmus and Webb, 1982 and Scott, 1986). In addition CPZ increased the percentage of active grafts and prolonged graft survival. The results suggest that CPZ has the ability to maintain tissue in culture while allowing for decreasing antigenicity. These findings may lead to a novel organ culture technique which will take advantage of Summerlin's reports in 1973 that culture of tissue prior to transplantation will increase allograft survival times. Furthermore recent reports that CPZ has immunosuppressive function could make this additive to tissue culture desirable when used prior to transplantation (Steele and Zacherie, 1988). |
| General note | Submitted to the faculty of the Department of Biology. |
| General note | Advisor: G. W. Kalmus |
| Dissertation note | M.S. East Carolina University 1990 |
| Bibliography note | Includes bibliographical references (leaves 38-43). |
| Genre/form | dissertations. |
| Genre/form | Academic theses. |
| Genre/form | Academic theses. |
| Genre/form | Thèses et écrits académiques. |