An evaluation of extracellular products of Staphylococcus aureus as possible agents increasing wound healing and wound breaking strength / by Gary Thomas Kucera.
| Author/creator | Kucera, Gary Thomas author. |
| Other author | Allen, Wendall E., degree supervisor. |
| Other author | East Carolina University. Department of Biology. |
| Format | Theses and dissertations |
| Production | 1990. |
| Description | [viii], 70 leaves : illustrations ; 28 cm |
| Supplemental Content | Access via ScholarShip |
| Subjects |
| Summary | Literature reports of accelerated wound healing promoted by Staphylococcus aureus #25904 bacteria were confirmed by inoculation of an 18-hour culture of this organism into surgical wounds on the backs of male Lewis and CD Fisher rats. No accelerated wound healing was observed when S. epidermidis #e155 was inoculated into the wounds. Surgical wounds treated with concentrated cell-free supernatant from S. aureus #25904 showed statistically significant increase in wound breaking strength. Treatment with concentrated total crude cell extract from the same organism did not. This suggested that the factor (s) responsible for accelerated wound healing are excreted products. Partially purified preparations of hyaluronidase and phosphatase, and a highly purified preparation of protein A, were tested individually for accelerated wound healing activity. Wounds treated with these individual preparations expressed no statistical differences from controls. Treatment with a mixture of all three resulted in a noticeable increase in wound breaking strength, suggesting either a synergistic effect or that additional factors were present in the mixture in sufficient amounts to elicit the increase in wound healing activity. |
| General note | Submitted to the faculty of the Department of Biology. |
| General note | Advisor: Wendall E. Allen |
| Dissertation note | M.S. East Carolina University 1990 |
| Bibliography note | Includes bibliographical references (leaves 67-70). |
| Genre/form | Academic theses. |
| Genre/form | Academic theses. |
| Genre/form | Thèses et écrits académiques. |