The effect of platelet activation inhibitors on the generation of microparticles in platelet concentrates stored for transfusion using flow cytometry / by Susan M. Orton.
| Author/creator | Orton, Susan M. author. |
| Other author | Kalmus, Gerhard W., degree supervisor. |
| Other author | East Carolina University. Department of Biology. |
| Format | Theses and dissertations |
| Production | 1990. |
| Description | [iv], 58 leaves : illustrations ; 28 cm |
| Supplemental Content | Access via ScholarShip |
| Subjects |
| Summary | Membranous microparticles appearing in the supernatant plasma of stored platelet concentrates were analyzed by flow cytometry. Two populations of microparticles were arbitrarily delineated by light scatter as larger or smaller than 0.5 [mu]m obtained by adding a known amount of fluorescent beads to each sample before analysis of a set number of counts on the flow cytometer. The addition of platelet activation inhibitors (Prostaglandin E-1, theophylline, and aprotinin) to the anticoagulant during preparation of platelet concentrates caused a 40% reduction in the number of microparticles appearing during storage relative to donor-matched controls. In addition, the inhibited concentrates had 84% less Platelet Factor 3 (PF3) activity in the supernatant and 61% less released lactic dehydrogenase. A significant correlation was found (rs=0.748) between PF3 levels and the concentration of larger microparticles. The inhibitors did not reduce the small number of microparticles found in stored platelet-poor plasma. Surface antigen analysis showed that the majority of microparticles in platelet concentrates were platelet-derived; most were positive for glycoproteinllb Ilia (73%) but not for glycoprotein Ib (30%) . These results indicate that procoagulant microparticles are released from platelets during storage as a result of platelet activation. |
| General note | Submitted to the faculty of the Department of Biology. |
| General note | Advisor: Gerhard W. Kalmus |
| Dissertation note | M.S. East Carolina University 1990 |
| Bibliography note | Includes bibliographical references (leaves 53-58). |
| Genre/form | dissertations. |
| Genre/form | Academic theses. |
| Genre/form | Academic theses. |
| Genre/form | Thèses et écrits académiques. |
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