Fragment-based drug design tools, practical approaches, and examples / edited by Lawrence C. Kuo.
| Other author | Kuo, Lawrence C. |
| Format | Electronic |
| Edition | 1st ed. |
| Publication Info | San Diego, Calif. : Elsevier/Academic Press, |
| Description | li, 591 p., [16] p. of plates : ill. (some col.) ; 24 cm. |
| Supplemental Content | Full text available from ScienceDirect Enzymology Book Series |
| Supplemental Content | Full text available from Methods in Enzymology |
| Subjects |
| Other title | Fragment based drug design |
| Series | Methods in enzymology, 0076-6879 ; vol. 493 Methods in enzymology ; v. 493. 0076-6879 ^A497553 |
| Contents | Designing a diverse high-quality library for crystallography-based FBDD screening -- Preparation of protein samples for NMR structure, function, and small-molecule screening studies -- Key factors for successful generation of protein-fragment structures requirement on protein, crystals, and technology -- Predicting the success of fragment screening by X-ray crystallography -- Fragment screening of stabilized G-protein-coupled receptors using biophysical methods -- Using computational techniques in fragment-based drug discovery -- How to avoid rediscovering the known -- From experimental design to validated hits a comprehensive walk-through of fragment lead identification using surface plasmon resonance -- Practical aspects of NMR-based fragment screening -- Binding site identification and structure determination of protein-ligand complexes by NMR a semiautomated approach -- Protein thermal shifts to identify low molecular weight fragments -- HTS reporter displacement assay for fragment screening and fragment evolution toward leads with optimized binding kinetics, binding selectivity, and thermodynamic signature -- Fragment screening purely with protein crystallography -- Computational approach to de novo discovery of fragment binding for novel protein states -- Lead generation and examples opinion regarding how to follow up hits -- Medicinal chemistry inspired fragment-based drug discovery -- Effective progression of nuclear magnetic resonance-detected fragment hits -- Advancing fragment binders to lead-like compounds using ligand and protein-based NMR spectroscopy -- Electron density guided fragment-based drug design--a lead generation example -- Experiences in fragment-based lead discovery -- Fragment screening of infectious disease targets in a structural genomics environment. |
| Bibliography note | Includes bibliographical references and indexes. |
| Access restriction | Available only to authorized users. |
| Technical details | Mode of access: World Wide Web |
| Genre/form | Electronic books. |
| ISBN | 9780123812742 |
| ISBN | 0123812747 |