Laboratory methods in enzymology. / edited by Jon Lorsch.

Other author Lorsch, Jon.
Format Electronic
EditionFirst edition.
Publication InfoAmsterdam ; Boston : Elsevier/Academic Press, 2013.
Descriptionlviii, 413 pages : illustrations ; 24 cm.
Supplemental ContentFull text available from Methods in Enzymology
Supplemental ContentFull text available from ScienceDirect Enzymology Book Series
Subjects
RNA--chemistry.
RNA--physiology.

Variant title RNA
SeriesMethods in enzymology, 0076-6879 ; volume 530
Methods in enzymology ; v. 530. 0076-6879 ^A497553
Contents Computational prediction of RNA secondary structure / Walter N. Moss -- Reverse-transcription PCR (RT-PCR) / Julia Bachman -- Northern blotting / Shan L. He and Rachel Green -- Explanatory chapter: nuclease protection assays / Elizabeth Eyler -- In vitro transcription from plasmid or PCR-amplified DNA / Julie L. Brunelle and Rachel Green -- Measurement of in vivo RNA synthesis rates / David S. McPheeters and Jo Ann Wise -- Method for measuring mRNA decay rate in Saccharomyces cerevisiae / Wenqian Hu and Jeff Coller -- Analysis of polysomes from bacteria / Daoming Qin and Kurt Fredrick -- Polysome profile analysis : yeast / Martin Pospi<U+0301>sek and Leos Vala<U+0301>sek -- Polysome analysis of mammalian cells / Shan L. He and Rachel Green -- Polysome analysis for determining mRNA and ribosome association in Saccharomyces cerevisiae / Wenqian Hu and Jeff Coller -- Oligo(dT)-primed RT-PCR isolation of polyadenylated RNA degradation intermediates / Shimyn Slomovic and Gadi Schuster -- Circularized RT-PCR (cRT-PCR) : analysis of the 5' ends, 3' ends, and poly(A) tails of RNA / Shimyn Slomovic and Gadi Schuster -- RNA radiolabeling / Rishi Porecha and Daniel Herschlag -- Fluorescently labeling synthetic RNAs / Max Greenfield and Daniel Herschlag -- Analysis of RNA by analytical polyacrylamide gel electrophoresis / Alexey Petrov, Albet Tsa, and Joseph D. Puglisi -- RNA purification by preparative polyacrylamide gel electrophoresis / Alexey Petrov, Tinghe Wu, Elisabetta Viani Puglisi, and Joseph D. Puglisi -- Explanatory chapter : nucleic acid concentration determination / Sarah Kolitz and Jon Lorsch -- RNA purification : precipitation methods / Sarah E. Walker and Jon Lorsch -- Reverse transcriptase dideoxy sequencing of RNA / Sarah E. Walker and Jon Lorsch -- RNA structure experimental analysis : chemical modification / Zhili Xu and Gloria Culver -- Structural analysis of RNA backbone using in-line probing / Ali Nahvi and Rachel Green.
Bibliography noteIncludes bibliographical references and indexes.
Access restrictionAvailable only to authorized users.
Other formsAlso issued online.
Technical detailsMode of access: World Wide Web
Genre/formElectronic books.
ISBN9780124200371
ISBN0124200370