Morphological evidence for a substance P projection from a medial septum to hippocampus / by Carol L. Surlow.
| Author/creator | Shurlow, Carol L. author. |
| Other author | Peterson, Gary M., degree supervisor. |
| Other author | East Carolina University. Department of Biology. |
| Format | Theses and dissertations |
| Production | 1991. |
| Description | [iv], 47 leaves : illustrations ; 28 cm |
| Supplemental Content | Access via ScholarShip |
| Subjects |
| Summary | The medial septal nucleus (MS) provides one of the major afferents to the hippocampal formation and the hippocampus projects back to the lateral septum. The two major types of neurons present in the MS are cholinergic and GABAergic, but other types of neurons are also present. A small population of substance P (SP) containing neurons are present along the border between the medial and lateral septum but it is unclear whether these project to the hippocampus. The present study, by employing both anterograde and retrograde tracing techniques, combined with immunocytochemistry for SP, provides direct morphological evidence for a SP projection from MS to the hippocampus. The anterogradely transported lectin PHA-L was iontophoretically injected into the vicinity of the SP-containing cells in the MS. Using the indirect fluorescent immunocytochemical method and two different fluorochromes, PHA-L and SP were co-localized within fibers in the CA2 region of the hippocampus but only in the mid septo-temporal portion. To provide confirmation of this projection, the retro gradely transported marker WGA/HRP was injected into the suspected region of termination of MS SP fibers in the hippocampus. Using two different colored chromagens, SP and WGA-HRP were co-localized in cell bodies in the lateral portion of the MS. Together, the evidence from anterograde and retrograde tracings demonstrate that the SP containing cells in the lateral region of MS project to a region of CA2 which is restricted to the mid septo-temporal portion of the hippocampus. |
| General note | Submitted to the faculty of the Department of Biology. |
| General note | Advisor: Gary M. Peterson |
| Dissertation note | M.S. East Carolina University 1991 |
| Bibliography note | Includes bibliographical references (leaves 42-47). |
| Genre/form | Academic theses. |
| Genre/form | Academic theses. |
| Genre/form | Thèses et écrits académiques. |
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