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LEADER 03755ctm 2200541 i 4500

001 ocm25693343;

003 OCoLC;

005 20250120151958.0;

008 920422s1991 xx a bm 000 0 eng d;

035 a| (Sirsi) o25693343;

035 a| (OCoLC)25693343;

049 a| EREE;

050 4 a| QP572.V3b| G87x 1991;

100 1 a| Gurganus, Thomas M.,e| author.?| UNAUTHORIZED;

245 10 a| Preliminary examination of proteins in extracts of sinus gland of the Blue Crab, Callinectes sapidus :b| indications of the presence of a vasopressin-like factor /c| by Thomas M. Gurganus.;

264 0 c| 1991.;

300 a| [vi], 66 pages, 17 unnumbered leaves :b| illustrations ;c| 28 cm;

336 a| textb| txt2| rdacontent;

337 a| unmediatedb| n2| rdamedia;

338 a| volumeb| nc2| rdacarrier;

502 b| M.S.c| East Carolina Universityd| 1991;

500 a| Submitted to the faculty of the Department of Biology.;

500 a| Advisor: Charles A. Singhas;

520 3 a| Previous studies have identified a number of proteins in extracts of the x-organ/sinus gland complex in a variety of Crustacea and other arthropods. In several species, substances similar to the vertebrate vasopressins have been reported. The goal of this study is : 1) to examine the distribution of proteins in extracts of blue crab sinus glands, and 2) to investigate the presence of vasopressin-like peptides in the sinus gland of the blue crab, Callinectes sapidus. Crabs were collected from the Pamlico and Albemarle Sounds of North Carolina. Eyestalks of the crabs were removed and stored at -70°C. Proteins were extracted by boiling in 0.1N HCl and filtering through Sep-Pak C-18 columns. Samples were then subjected to HPLC and SDS-PAGE. Other eyestalks were dissected to remove the sinus glands which were then extracted as above. Extracts were subjected to SDS-PAGE and HPLC. Results of these experiments indicate the presence of 11 eyestalk proteins. Sinus gland extracts contained a factor that co-migrated on SDS-PAGE and co-eluted from HPLC with lysine- and arginine-vasopressin. Utilizing the criteria established by Mattson & Spaziani (1985) and others, it is possible that this factor is a blue crab molt-inhibiting hormone (MIH).;

504 a| Includes bibliographical references (leaves 48-54).;

650 0 a| Vasopressin.=| ^A5883;

650 0 a| Crustaceax| Anatomy.=| ^A70779;

650 0 a| Endocrinology, Experimental.=| ^A4804;

650 2 a| Vasopressins.0| (DNLM)D014667=| ^A924454;

655 2 a| Academic Dissertation.0| (DNLM)D019478?| UNAUTHORIZED;

650 7 a| Crustaceax| Anatomy.2| fast0| (OCoLC)fst00884441;

650 7 a| Endocrinology, Experimental.2| fast0| (OCoLC)fst00909762;

650 7 a| Vasopressin.2| fast0| (OCoLC)fst01164595;

655 7 a| Academic theses.2| fast0| (OCoLC)fst01726453;

655 7 a| Academic theses.2| lcgft;

655 7 a| Thèses et écrits académiques.2| rvmgf0| (CaQQLa)RVMGF-000001173;

700 1 a| Singhas, Charles A.,e| degree supervisor.?| UNAUTHORIZED;

710 2 a| East Carolina University.b| Department of Biology.=| ^A637467;

856 41 z| Access via ScholarShipu| http://hdl.handle.net/10342/12045;

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Preliminary examination of proteins in extracts of sinus gland of the Blue Crab, Callinectes sapidus : indications of the presence of a vasopressin-like factor / by Thomas M. Gurganus.

Author/creator	Gurganus, Thomas M. author.
Other author	Singhas, Charles A., degree supervisor.
Other author	East Carolina University. Department of Biology.
Format	Theses and dissertations
Production	1991.
Description	[vi], 66 pages, 17 unnumbered leaves : illustrations ; 28 cm
Supplemental Content	Access via ScholarShip
Subjects	Vasopressin. Crustacea--Anatomy. Endocrinology, Experimental. Vasopressins.

Summary	Previous studies have identified a number of proteins in extracts of the x-organ/sinus gland complex in a variety of Crustacea and other arthropods. In several species, substances similar to the vertebrate vasopressins have been reported. The goal of this study is : 1) to examine the distribution of proteins in extracts of blue crab sinus glands, and 2) to investigate the presence of vasopressin-like peptides in the sinus gland of the blue crab, Callinectes sapidus. Crabs were collected from the Pamlico and Albemarle Sounds of North Carolina. Eyestalks of the crabs were removed and stored at -70°C. Proteins were extracted by boiling in 0.1N HCl and filtering through Sep-Pak C-18 columns. Samples were then subjected to HPLC and SDS-PAGE. Other eyestalks were dissected to remove the sinus glands which were then extracted as above. Extracts were subjected to SDS-PAGE and HPLC. Results of these experiments indicate the presence of 11 eyestalk proteins. Sinus gland extracts contained a factor that co-migrated on SDS-PAGE and co-eluted from HPLC with lysine- and arginine-vasopressin. Utilizing the criteria established by Mattson & Spaziani (1985) and others, it is possible that this factor is a blue crab molt-inhibiting hormone (MIH).
General note	Submitted to the faculty of the Department of Biology.
General note	Advisor: Charles A. Singhas
Dissertation note	M.S. East Carolina University 1991
Bibliography note	Includes bibliographical references (leaves 48-54).
Genre/form	Academic theses.
Genre/form	Academic theses.
Genre/form	Thèses et écrits académiques.

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Preliminary examination of proteins in extracts of sinus gland of the Blue Crab, Callinectes sapidus : indications of the presence of a vasopressin-like factor / by Thomas M. Gurganus.

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