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LEADER 03754ctm 2200541 i 4500

001 ocm25749560;

003 OCoLC;

005 20251021154322.0;

008 920501s1973 xx a bm 000 0 eng d;

035 a| (Sirsi) o25749560;

035 a| (OCoLC)25749560;

049 a| EREE;

050 4 a| QP613.A33b| W37x 1973;

100 1 a| Ward, Denise Kornegay,e| author.?| UNAUTHORIZED;

245 10 a| Some studies on adenyl cyclase /c| by Denise Kornegay Ward.;

264 0 c| 1973.;

300 a| 54 leaves :b| illustrations ;c| 28 cm;

336 a| textb| txt2| rdacontent;

337 a| unmediatedb| n2| rdamedia;

338 a| volumeb| nc2| rdacarrier;

502 b| M.A.c| East Carolina Universityd| 1973;

500 a| Submitted to the faculty of the Department of Biology.;

500 a| Advisor: Sam N. Pennington;

520 3 a| Adenyl cyclase from several mammalian as well as bacterial sources was studied. Highest specific activity was observed in rat cerebral cortical tissue and lowest specific activity was noted in bacteria. An increase in specific activity was noted when hormones and fluoride ion were added to the animal tissue. F~ also caused an increase in the specific activity of the bacterial cyclase but hormones elicited a decrease. The detergent Luberol-PX proved to be an effective solubilizing agent for adenyl cyclase from animal tissue. This detergent solubilized adenyl cyclase not only from heart tissue, as previously reported, but also solubilized the enzyme from brain and liver tissue. The solubilized tissue, in agreement with previous work, did not show normal hormonal response. The detergent was removed using a DEAE cellulose column. No activity could be recovered for brain adenyl cyclase from the column. Heart specific activity was higher off the column than before being placed on the column; however, the normal hormonal response was still not present and with the addition of the hormones there was a decrease in specific activity. Phospholipids did in some cases increase the specific activity over that of enzyme and hormone together. The bacterial adenyl cyclase was Inhibited by the addition of hormones. Phospholipids plus hormones increased the specific activity over that where only hormones were present.;

504 a| Includes bibliographical references (leaves 50-51).;

650 0 a| Adenylate cyclase.=| ^A286091;

650 0 a| Enzymes.=| ^A458;

650 2 a| Adenylyl Cyclases.0| (DNLM)D000262=| ^A948072;

650 2 a| Enzymes.0| (DNLM)D004798=| ^A917771;

655 2 a| Academic Dissertation.0| (DNLM)D019478?| UNAUTHORIZED;

650 7 a| enzyme.2| aat0| (CStmoGRI)aat300212647;

650 7 a| Adenylate cyclase.2| fast0| (OCoLC)fst00796589;

650 7 a| Enzymes.2| fast0| (OCoLC)fst00913605;

655 7 a| Academic theses.2| fast0| (OCoLC)fst01726453;

655 7 a| Academic theses.2| lcgft;

655 7 a| Thèses et écrits académiques.2| rvmgf0| (CaQQLa)RVMGF-000001173;

700 1 a| Pennington, Sam N.,e| degree supervisor.?| UNAUTHORIZED;

710 2 a| East Carolina University.b| Department of Biology.=| ^A637467;

856 41 z| Access via ScholarShipu| http://hdl.handle.net/10342/11579;

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949 a| Click on web addressw| asish| hsl111;

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998 a| 416943;

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Some studies on adenyl cyclase / by Denise Kornegay Ward.

Author/creator	Ward, Denise Kornegay author.
Other author	Pennington, Sam N., degree supervisor.
Other author	East Carolina University. Department of Biology.
Format	Theses and dissertations
Production	1973.
Description	54 leaves : illustrations ; 28 cm
Supplemental Content	Access via ScholarShip
Subjects	Adenylate cyclase. Enzymes. Adenylyl Cyclases. Enzymes.

Summary	Adenyl cyclase from several mammalian as well as bacterial sources was studied. Highest specific activity was observed in rat cerebral cortical tissue and lowest specific activity was noted in bacteria. An increase in specific activity was noted when hormones and fluoride ion were added to the animal tissue. F~ also caused an increase in the specific activity of the bacterial cyclase but hormones elicited a decrease. The detergent Luberol-PX proved to be an effective solubilizing agent for adenyl cyclase from animal tissue. This detergent solubilized adenyl cyclase not only from heart tissue, as previously reported, but also solubilized the enzyme from brain and liver tissue. The solubilized tissue, in agreement with previous work, did not show normal hormonal response. The detergent was removed using a DEAE cellulose column. No activity could be recovered for brain adenyl cyclase from the column. Heart specific activity was higher off the column than before being placed on the column; however, the normal hormonal response was still not present and with the addition of the hormones there was a decrease in specific activity. Phospholipids did in some cases increase the specific activity over that of enzyme and hormone together. The bacterial adenyl cyclase was Inhibited by the addition of hormones. Phospholipids plus hormones increased the specific activity over that where only hormones were present.
General note	Submitted to the faculty of the Department of Biology.
General note	Advisor: Sam N. Pennington
Dissertation note	M.A. East Carolina University 1973
Bibliography note	Includes bibliographical references (leaves 50-51).
Genre/form	Academic theses.
Genre/form	Academic theses.
Genre/form	Thèses et écrits académiques.

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