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LEADER 03699ctm 2200553 i 4500

001 ocm25749497;

003 OCoLC;

005 20240430153507.0;

008 920501s1974 xx a bm 000 0 eng d;

035 a| (Sirsi) o25749497;

035 a| (OCoLC)25749497;

049 a| EREE;

050 4 a| QR92.S68b| C64x 1974;

100 1 a| Cole, Joseph Jerald,c| III,e| author.?| UNAUTHORIZED;

245 10 a| Chromosomal differences between cultures of Staphylococcus aureus /c| Joseph Jerald Cole III.;

264 0 c| 1974.;

300 a| 54 leaves :b| illustrations ;c| 28 cm;

336 a| textb| txt2| rdacontent;

337 a| unmediatedb| n2| rdamedia;

338 a| volumeb| nc2| rdacarrier;

502 b| M.A.c| East Carolina Universityd| 1974;

500 a| Submitted to the faculty of the Department of Biology.;

500 a| Advisor: Wendall E. Allen;

520 3 a| Genomic maps of two strains of Staphylococcus aureus, 7-8 (animal origin) and 2 (human origin), were prepared by determining the replication sequence of streptomycin, puromycin, chloramphenicol, neomycin, and mitomycin C-resistance factors by the synchronous chromosomal replication technique. Nitrosoguanidine was used for mutagenesis and chromosomal replication synchronized by treatment with phenethyl alcohol. The order of replication of the genes tested was the same in both strains of aureus. Genes replicating at similar times in both strains showed similar distances between genes. The genes for streptomycin and puromycin resistance, replicating near the origin of chromosomal replication in strain 7-8 and near the terminus in strain 2, had different times of replication and distances between genes. The position of a gene determined by synchronous chromosomal replication was shown not to vary with concentration, pH, or source of the mutagen. The numbers of mutants obtained after the gene in question had replicated were different with different pH of the solution of nitrosoguanidine. The point of initiation and the direction of chromosomal replication were different for the two strains of S. aureus. Mechanisms were proposed to explain these differences.;

504 a| Includes bibliographical references (leaves 49-54).;

650 0 a| Staphylococcus aureus.=| ^A250296;

650 0 a| Bacteria.=| ^A3199;

650 0 a| Bacterial genetics.=| ^A5320;

650 2 a| Staphylococcus aureus0| (DNLM)D013211=| ^A1204094;

650 2 a| Bacteria.0| (DNLM)D001419=| ^A918694;

655 2 a| Academic Dissertation.0| (DNLM)D019478?| UNAUTHORIZED;

650 7 a| Bacteria.2| fast0| (OCoLC)fst00825152;

650 7 a| Bacterial genetics.2| fast0| (OCoLC)fst00825221;

650 7 a| Staphylococcus aureus.2| fast0| (OCoLC)fst01131544;

655 7 a| Academic theses.2| fast0| (OCoLC)fst01726453;

655 7 a| Academic theses.2| lcgft;

655 7 a| Thèses et écrits académiques.2| rvmgf0| (CaQQLa)RVMGF-000001173;

700 1 a| Allen, Wendall E.,e| degree supervisor.?| UNAUTHORIZED;

710 2 a| East Carolina University.b| Department of Biology.=| ^A637467;

856 40 z| Access via ScholarShipu| http://hdl.handle.net/10342/11633;

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Chromosomal differences between cultures of Staphylococcus aureus / Joseph Jerald Cole III.

Author/creator	Cole, Joseph Jerald author.
Other author	Allen, Wendall E., degree supervisor.
Other author	East Carolina University. Department of Biology.
Format	Theses and dissertations
Production	1974.
Description	54 leaves : illustrations ; 28 cm
Supplemental Content	Access via ScholarShip
Subjects	Staphylococcus aureus. Bacteria. Bacterial genetics. Staphylococcus aureus Bacteria.

Summary	Genomic maps of two strains of Staphylococcus aureus, 7-8 (animal origin) and 2 (human origin), were prepared by determining the replication sequence of streptomycin, puromycin, chloramphenicol, neomycin, and mitomycin C-resistance factors by the synchronous chromosomal replication technique. Nitrosoguanidine was used for mutagenesis and chromosomal replication synchronized by treatment with phenethyl alcohol. The order of replication of the genes tested was the same in both strains of aureus. Genes replicating at similar times in both strains showed similar distances between genes. The genes for streptomycin and puromycin resistance, replicating near the origin of chromosomal replication in strain 7-8 and near the terminus in strain 2, had different times of replication and distances between genes. The position of a gene determined by synchronous chromosomal replication was shown not to vary with concentration, pH, or source of the mutagen. The numbers of mutants obtained after the gene in question had replicated were different with different pH of the solution of nitrosoguanidine. The point of initiation and the direction of chromosomal replication were different for the two strains of S. aureus. Mechanisms were proposed to explain these differences.
General note	Submitted to the faculty of the Department of Biology.
General note	Advisor: Wendall E. Allen
Dissertation note	M.A. East Carolina University 1974
Bibliography note	Includes bibliographical references (leaves 49-54).
Genre/form	Academic theses.
Genre/form	Academic theses.
Genre/form	Thèses et écrits académiques.

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